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    METHOD OF SEPARATING NUCLEIC ACIDS
    3.
    发明申请
    METHOD OF SEPARATING NUCLEIC ACIDS 审中-公开
    分离核酸的方法

    公开(公告)号：US20150044725A1

    公开(公告)日：2015-02-12

    申请号：US14222314

    申请日：2014-03-21

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Chang-eun YOO ,  Yeon-jeong Kim ,  Dong-hyun Park ,  Kyung-yeon Han

    IPC: C12Q1/68

    CPC classification number: C12N15/1013 ,  C12N15/1006

    Abstract: A method of separating nucleic acids from cells, the method comprising incubating a sample comprising cells with a solid substrate that binds to the cells, whereby the cells adhere to the solid substrate; suspending the solid substrate adhered to the cells in a lysis composition comprising about 100 mM to about 300 mM of alkaline metal salt, and having a pH of about 6 to about 8; lysing the cells in the lysis composition to obtain a lysed solution; and obtaining the nucleic acids from the lysed solution; as well as related compositions and kits.

    Abstract translation: 一种从细胞中分离核酸的方法，所述方法包括将包含细胞的样品与固定于细胞上的固体底物孵育，由此所述细胞粘附到固体基质上; 将固体底物悬浮在包含约100mM至约300mM碱金属盐并且pH为约6至约8的裂解组合物中的细胞上; 裂解裂解组合物中的细胞以获得裂解溶液; 从裂解的溶液中获得核酸; 以及相关组合物和试剂盒。

    Method of detecting nucleic acids containing a genetic variation
    4.
    发明授权
    Method of detecting nucleic acids containing a genetic variation 有权
    检测含有遗传变异的核酸的方法

    公开(公告)号：US09523118B2

    公开(公告)日：2016-12-20

    申请号：US14189762

    申请日：2014-02-25

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Dong-hyun Park ,  Yeon-jeong Kim ,  Kyung-yeon Han

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2537/163

    Abstract: Provided are a composition or a kit for detecting a nucleic acid with genetic variation including a first amplification blocking nucleic acid and a second amplification blocking nucleic acid, and a method of detecting a nucleic acid with genetic variation by using the same. Based on the above, a nucleic acid with genetic variation can be detected with high sensitivity and accuracy.

    Abstract translation: 提供了用于检测具有遗传变异的核酸的组合物或试剂盒，包括第一扩增阻断核酸和第二扩增阻断核酸，以及通过使用该核酸检测具有遗传变异的核酸的方法。 基于上述，可以以高灵敏度和准确度检测出具有遗传变异的核酸。

    METHOD FOR REVERSIBLE FIXATION OR SELECTIVE LYSIS OF CELL USING PHOTOCLEAVABLE POLYMER
    5.
    发明申请
    METHOD FOR REVERSIBLE FIXATION OR SELECTIVE LYSIS OF CELL USING PHOTOCLEAVABLE POLYMER 有权
    使用光可溶性聚合物进行反向固定或选择性细胞分析的方法

    公开(公告)号：US20150148243A1

    公开(公告)日：2015-05-28

    申请号：US14323812

    申请日：2014-07-03

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Hun-joo LEE ,  Yeon-jeong Kim ,  Dong-hyun Park ,  Jong-myeon Park

    IPC: G01N1/30

    CPC classification number: G01N1/30 ,  G01N2001/305

    Abstract: A photocleavable polymer comprising a photocleavable linker and a polymer having a functional group that binds to a protein, lipid, sugar of a cell, or any combination thereof, as well as a method of selectively lysing cells by incubating a sample comprising two or more cells with the photocleavable polymer to reversibly fix the cells; selectively irradiating the fixed cells or a target or non-target cell among the fixed cells to cleave the photocleavable polymer; and adding a cell lysis solution to selectively lyse the irradiated cells.

    Abstract translation: 包含可光切割接头和具有结合蛋白质，脂质，细胞糖或其任何组合的官能团的聚合物的可光致透照聚合物，以及通过将包含两个或多个细胞的样品 用可透光聚合物可逆地固定细胞; 选择性地照射固定细胞中的固定细胞或靶或非靶细胞以切割可光裂解的聚合物; 并加入细胞裂解溶液以选择性地裂解照射的细胞。

    Cell capturing filter having high aspect ratio
    8.
    发明授权
    Cell capturing filter having high aspect ratio 有权
    具有高纵横比的细胞捕获过滤器

    公开(公告)号：US09322755B2

    公开(公告)日：2016-04-26

    申请号：US13713678

    申请日：2012-12-13

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Tae-seok Sim ,  Min-seoks Kim ,  Mi-jeong Song ,  Jeong-gun Lee ,  June-young Lee ,  Yeon-jeong Kim ,  Sang-hyun Baek ,  Jin-mi Oh ,  Hyo-young Jeong

    IPC: G01N1/34 ,  B01L3/00 ,  G01N1/40 ,  C12M1/26 ,  C12M1/00

    CPC classification number: G01N1/34 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/086 ,  C12M33/14 ,  C12M47/04 ,  G01N1/4077

    Abstract: A cell capturing filter, in which cells or particles having a predetermined size or greater in a sample may be easily captured, and clogging of a flow passage due to the captured cells or particles may be prevented, and stresses acting on the captured cells or particles may be reduced, having a first surface and a second substrate having a second surface that is bonded to the first surface, wherein the first substrate may include a flow passage formed in the first surface of the first substrate so that a sample flows in the flow passage, and a barrier that protrudes across the flow passage, and the second substrate may include a fine groove formed in an area on the second surface corresponding to the barrier, and a gap may exist between a surface of the barrier and the fine groove.

    Abstract translation: 可以容易地捕获其中容易捕获样品中具有预定尺寸或更大尺寸的细胞或颗粒的细胞捕获过滤器，并且可以防止由于捕获的细胞或颗粒引起的流动通道的堵塞，并且可以防止作用在捕获的细胞或颗粒上的应力 可以减小，具有第一表面和第二基底，其具有结合到第一表面的第二表面，其中第一基底可以包括形成在第一基底的第一表面中的流动通道，使得样品在流动中流动 通道和横过流道突出的屏障，并且第二基板可以包括形成在对应于屏障的第二表面上的区域中的细槽，并且在屏障的表面和细槽之间可能存在间隙。

    METHOD OF AMPLIFYING NUCLEIC ACID
    9.
    发明申请
    METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
    放大核酸的方法

    公开(公告)号：US20150037801A1

    公开(公告)日：2015-02-05

    申请号：US14180931

    申请日：2014-02-14

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Dong-hyun PARK ,  Yeon-jeong Kim ,  Kyung-yeon Han

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2521/537 ,  C12Q2523/301

    Abstract: Provided is a method of amplifying a nucleic acid of a cell involving applying ultrasonic waves to lyse a cell bound to a solid support to provide a cell lysate comprising a nucleic acid; separating the cell lysate from the solid support; adding a protease to the cell lysate; and amplifying the nucleic acid.

    Abstract translation: 提供一种扩增细胞核酸的方法，所述方法包括施加超声波以裂解与固体支持物结合的细胞，以提供包含核酸的细胞裂解物; 将细胞裂解物与固体支持物分离; 向细胞裂解物中加入蛋白酶; 并扩增核酸。

    METHOD OF DETECTING NUCLEIC ACIDS CONTAINING A GENETIC VARIATION
    10.
    发明申请
    METHOD OF DETECTING NUCLEIC ACIDS CONTAINING A GENETIC VARIATION 有权
    检测含有遗传变异的核酸的方法

    公开(公告)号：US20150031023A1

    公开(公告)日：2015-01-29

    申请号：US14189762

    申请日：2014-02-25

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Dong-hyun PARK ,  Yeon-jeong Kim ,  Kyung-yeon Han

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2537/163

    Abstract: Provided are a composition or a kit for detecting a nucleic acid with genetic variation including a first amplification blocking nucleic acid and a second amplification blocking nucleic acid, and a method of detecting a nucleic acid with genetic variation by using the same. Based on the above, a nucleic acid with genetic variation can be detected with high sensitivity and accuracy.

    Abstract translation: 提供了用于检测具有遗传变异的核酸的组合物或试剂盒，包括第一扩增阻断核酸和第二扩增阻断核酸，以及通过使用该核酸检测具有遗传变异的核酸的方法。 基于上述，可以以高灵敏度和准确度检测出具有遗传变异的核酸。

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