公开(公告)号：US07932046B2

公开(公告)日：2011-04-26

申请号：US12317954

申请日：2008-12-31

申请人： Sang Jun Sim ,  Jun Pyo Kim ,  Cheol Hee Park

发明人： Sang Jun Sim ,  Jun Pyo Kim ,  Cheol Hee Park

IPC分类号： C12M1/34 ,  G01N21/00 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5432 ,  Y10S435/808 ,  Y10S436/829 ,  Y10S436/905

摘要： Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

摘要翻译： 提供了生物材料检测方法，生物材料检测用芯片的制造方法以及生物材料检测用芯片。 检测生物材料的方法的特征在于包括以下步骤：（S1）将聚二乙炔脂质体固定在基材上; （S2）将聚二乙炔脂质体连接在一起并将它们层叠在基材上; （S3）将与要检测的受试生物材料形成互补结合的材料固定在聚二乙炔脂质体上; （S4）将得到的聚二乙炔脂质体暴露于紫外光，形成用于生物材料检测的芯片; （S5）将要检测的对象生物材料应用于用于反应的生物材料检测用芯片; 和（S6）测量来自用于生物材料检测的芯片的荧光信号。

公开(公告)号：US20120028267A1

公开(公告)日：2012-02-02

申请号：US13060177

申请日：2009-08-21

申请人： Sang Jun Sim ,  Jun Pyo Kim

发明人： Sang Jun Sim ,  Jun Pyo Kim

IPC分类号： G01N27/00 ,  C12N9/99 ,  C07K14/00 ,  C07H99/00 ,  C07H1/00 ,  C07K1/107 ,  C07K16/00 ,  C07K2/00 ,  B82Y5/00 ,  B82Y15/00

CPC分类号： G01N33/54353 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/174 ,  G01N33/54373 ,  G01N33/54393

摘要： Disclosed is a method of detecting even a very small amount of a target substance by mixing a linker and a spacer at a suitable ratio and immobilizing the mixture on the surface of carbon nanotubes in a carbon nanotube-based biosensor. This method detects a specific substance at the level of femtomoles and lowers the detection limit of conventional carbon nanotube transistor sensors. Accordingly, the method detects even a very small amount of a target substance, and thus the carbon nanotube-based biosensor is a highly useful sensor which can be used either as a medical sensor for diagnosing diseases or as an environmental sensor.

摘要翻译： 公开了通过以适当的比例混合接头和间隔物来均匀检测目标物质的方法，并将该混合物固定在碳纳米管基生物传感器中的碳纳米管表面上。 该方法可以检测飞镖水平的特定物质，降低常规碳纳米管晶体管传感器的检测限。 因此，该方法即使检测到极少量的目标物质，因此基于碳纳米管的生物传感器是可用作诊断疾病的医疗传感器或作为环境传感器的高度有用的传感器。

公开(公告)号：US20110014724A1

公开(公告)日：2011-01-20

申请号：US12867355

申请日：2008-05-26

申请人： Sang Jun Sim ,  Jun Pyo Kim ,  Cuong Cao

发明人： Sang Jun Sim ,  Jun Pyo Kim ,  Cuong Cao

IPC分类号： G01N33/553 ,  B05D1/00 ,  B05D3/10

CPC分类号： G01N33/54346 ,  G01N33/54373

摘要： Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.

摘要翻译： 公开了使用金纳米颗粒的局部表面等离子体共振（LSPR）检测生物产物的方法，其可以在将金纳米颗粒固定在玻璃面板上之后基于由抗原 - 抗体反应发生的最大波长的变化来诊断生物产物。 使用这种方法的传感器具有高灵敏度，价格低廉并且使得快速诊断成为可能，从而适用于与环境污染物，病原体等相关的各种生物领域以及疾病的诊断。 此外，与使用SPR的常规方法相比，它提供了用于制造具有更高灵敏度，低价格和快速性能的传感器的技术。

公开(公告)号：US20090291454A1

公开(公告)日：2009-11-26

申请号：US12317954

申请日：2008-12-31

申请人： Sang Jun Sim ,  Jun-Pyo Kim ,  Cheol-Hee Park

发明人： Sang Jun Sim ,  Jun-Pyo Kim ,  Cheol-Hee Park

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N21/76 ,  C12M1/00

CPC分类号： G01N33/5432 ,  Y10S435/808 ,  Y10S436/829 ,  Y10S436/905

摘要： Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

摘要翻译： 提供了生物材料检测方法，生物材料检测用芯片的制造方法以及生物材料检测用芯片。 检测生物材料的方法的特征在于包括以下步骤：（S1）将聚二乙炔脂质体固定在基材上; （S2）将聚二乙炔脂质体连接在一起并将它们层叠在基材上; （S3）将与要检测的受试生物材料形成互补结合的材料固定在聚二乙炔脂质体上; （S4）将得到的聚二乙炔脂质体暴露于紫外光，形成用于生物材料检测的芯片; （S5）将要检测的对象生物材料应用于用于反应的生物材料检测用芯片; 和（S6）测量来自用于生物材料检测的芯片的荧光信号。

公开(公告)号：US10041028B2

公开(公告)日：2018-08-07

申请号：US13995768

申请日：2011-01-20

申请人： Sang Jun Sim ,  Eun Hye Kim

发明人： Sang Jun Sim ,  Eun Hye Kim

IPC分类号： C12M1/00

摘要： The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film.The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof. Therefore, the photobioreactor of the present invention can be easily installed anywhere carbon dioxide is discharged, such as around a power-generating plant, in an urban region, a farm, etc., to culture a variety of photosynthetic organisms, and thus to produce useful substances having economically high added values.

公开(公告)号：US20130309762A1

公开(公告)日：2013-11-21

申请号：US13995768

申请日：2011-01-20

申请人： Sang Jun Sim ,  Eun Hye Kim

发明人： Sang Jun Sim ,  Eun Hye Kim

IPC分类号： C12M1/00

CPC分类号： C12M21/02 ,  C12M23/14 ,  C12M23/22 ,  C12M23/34

摘要： The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film.The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof. Therefore, the photobioreactor of the present invention can be easily installed anywhere carbon dioxide is discharged, such as around a power-generating plant, in an urban region, a farm, etc., to culture a variety of photosynthetic organisms, and thus to produce useful substances having economically high added values.

摘要翻译： 光生物反应器技术领域本发明涉及一种光生物反应器，更具体地，涉及一种用于培养利用二氧化碳和光能进行光合作用的微藻等生物体的光生物反应器。 光生物反应器包括：（a）由光合生物发生光合作用的反应容器; （b）形成在反应容器的外部上端的多用途入口/出口; （c）连接到反应容器外部的多功能入口/出口的外管; 和（d）连接到反应容器内部的多功能入口/出口的内管，其中反应容器由透明膜制成。 根据本发明的光生物反应器的优点在于其中发生光合作用的反应容器是板式的并且由透明膜制成，因此实现了改善的透光率和迁移率，并且使得其经济上有利的制造和操作。 因此，本发明的光生物反应器可以容易地安装在诸如在发电厂周围，城市地区，农场等中排放的二氧化碳的任何地方，以培养各种光合生物，从而产生 具有经济高附加值的有用物质。

公开(公告)号：US06409983B1

公开(公告)日：2002-06-25

申请号：US09614160

申请日：2000-07-11

申请人： Guang Jin Choi ,  Kyungja Woo ,  Young Sang Cho ,  Sang Jun Sim ,  Young Dae Kim ,  Sang Kyun Lee

发明人： Guang Jin Choi ,  Kyungja Woo ,  Young Sang Cho ,  Sang Jun Sim ,  Young Dae Kim ,  Sang Kyun Lee

IPC分类号： C01G2300

CPC分类号： B82Y30/00 ,  C01G23/006 ,  C01P2002/34 ,  C01P2002/72 ,  C01P2004/03 ,  C01P2004/32 ,  C01P2004/64

摘要： The present invention provides a process of preparing barium titanate powders having fine particle morphology and superior crystallinity, by preparing a sol precursor by using a titanium acylate and a barium compound, spraying the sol precursor in a strong alkaline solution for coprecipitation, crystallizing the barium titanate with an optional hydrothermal reaction, and purifying the barium titanate powder by washing.

摘要翻译： 本发明提供一种制备具有细小颗粒形态和优异结晶度的钛酸钡粉末的方法，通过使用钛酸酯和钡化合物制备溶胶前体，将溶胶前体喷在强碱溶液中共沉淀，使钛酸钡结晶 通过可选的水热反应，并通过洗涤来纯化钛酸钡粉末。

公开(公告)号：US5811272A

公开(公告)日：1998-09-22

申请号：US687806

申请日：1996-07-26

申请人： Kristi D. Snell ,  Scott A. Hogan ,  Sang Jun Sim ,  Anthony J. Sinskey ,  Chokyun Rha

发明人： Kristi D. Snell ,  Scott A. Hogan ,  Sang Jun Sim ,  Anthony J. Sinskey ,  Chokyun Rha

IPC分类号： C12N9/00 ,  C12P7/62 ,  C12N1/00 ,  C12N5/02 ,  C12P7/52

CPC分类号： C12N9/93 ,  C12P7/625

摘要： A method has been developed for control of molecular weight and molecular weight dispersity during production of polyhydroxyalkanoates in genetically engineered organism by control of the level and time of expression of one or more PHA synthases in the organisms. The method was demonstrated by constructing a synthetic operon for PHA production in E. coli in which the level of PHA synthase activity could be tightly controlled by placement of the synthase behind an inducible promoter. Modulation of the total level of PHA synthase activity in the host cell by varying the concentration of the inducer, isopropyl .beta.-D-thiogalactoside (IPTG), was found to effect the molecular weight of the polymer produced in the cell. Specifically, high concentrations of synthase activity were found to yield polymers of low molecular weight while low concentrations of synthase activity yielded polymers of higher molecular weight. Polymer molecular weight dispersity is also proportional to the amount of synthase activity, with less dispersity in polyhydroxyalkanoate compositions produced in expression systems with an initial burst of synthase activity, and higher levels of molecular weight dispersity in polyhydroxyalkanoate compositions produced in expression systems with the levels of synthase activity varied during synthesis of the polyhydroxyalkanoate.

摘要翻译： 已经开发了一种通过控制生物体内一种或多种PHA合成酶的表达水平和时间来控制在遗传工程生物体内聚羟基链烷酸酯生产过程中的分子量和分子量分散性的方法。 该方法通过在大肠杆菌中构建用于PHA生产的合成操纵子来证明，其中通过将合酶置于诱导型启动子后面可以严格控制PHA合酶活性的水平。 发现通过改变诱导剂异丙基β-D-硫代半乳糖苷（IPTG）的浓​​度来调节宿主细胞中PHA合成酶活性的总水平，从而影响细胞中产生的聚合物的分子量。 具体地说，发现高浓度的合成酶活性产生低分子量的聚合物，而低浓度的合酶活性则产生较高分子量的聚合物。 聚合物分子量分散度也与合成酶活性的量成比例，在具有合成酶活性的初始爆发的表达系统中产生的聚羟基链烷酸酯组合物的分散性较小，并且在表达系统中产生的聚羟基链烷酸酯组合物中分子量分散度的水平较高 合成酶活性在聚羟基链烷酸酯的合成过程中变化。