公开(公告)号：US07932046B2

公开(公告)日：2011-04-26

申请号：US12317954

申请日：2008-12-31

申请人： Sang Jun Sim ,  Jun Pyo Kim ,  Cheol Hee Park

发明人： Sang Jun Sim ,  Jun Pyo Kim ,  Cheol Hee Park

IPC分类号： C12M1/34 ,  G01N21/00 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5432 ,  Y10S435/808 ,  Y10S436/829 ,  Y10S436/905

摘要： Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

摘要翻译： 提供了生物材料检测方法，生物材料检测用芯片的制造方法以及生物材料检测用芯片。 检测生物材料的方法的特征在于包括以下步骤：（S1）将聚二乙炔脂质体固定在基材上; （S2）将聚二乙炔脂质体连接在一起并将它们层叠在基材上; （S3）将与要检测的受试生物材料形成互补结合的材料固定在聚二乙炔脂质体上; （S4）将得到的聚二乙炔脂质体暴露于紫外光，形成用于生物材料检测的芯片; （S5）将要检测的对象生物材料应用于用于反应的生物材料检测用芯片; 和（S6）测量来自用于生物材料检测的芯片的荧光信号。

公开(公告)号：US10041028B2

公开(公告)日：2018-08-07

申请号：US13995768

申请日：2011-01-20

申请人： Sang Jun Sim ,  Eun Hye Kim

发明人： Sang Jun Sim ,  Eun Hye Kim

IPC分类号： C12M1/00

摘要： The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film.The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof. Therefore, the photobioreactor of the present invention can be easily installed anywhere carbon dioxide is discharged, such as around a power-generating plant, in an urban region, a farm, etc., to culture a variety of photosynthetic organisms, and thus to produce useful substances having economically high added values.

公开(公告)号：US07785823B2

公开(公告)日：2010-08-31

申请号：US11677302

申请日：2007-02-21

申请人： Sang-jun Sim ,  Chang-deok Kang

发明人： Sang-jun Sim ,  Chang-deok Kang

IPC分类号： C12P23/00

CPC分类号： C07C403/24 ,  C12P23/00

摘要： There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2).

摘要翻译： 提供了一种从绿藻中选择性分离游离虾青素的方法，更具体地说，涉及一种从红潮球菌中选择性分离游离虾青素的方法，该方法包括：将含有积雪球菌的细菌培养物与烷烃溶剂混合并搅拌， 从而得到含有游离虾青素和虾青素的虾青素的烷烃溶剂提取物（步骤1）。 并将该烷醇溶剂提取物与醇混合并搅拌，从而得到含有游离虾青素的醇提取物（步骤2）。

公开(公告)号：US20130309762A1

公开(公告)日：2013-11-21

申请号：US13995768

申请日：2011-01-20

申请人： Sang Jun Sim ,  Eun Hye Kim

发明人： Sang Jun Sim ,  Eun Hye Kim

IPC分类号： C12M1/00

CPC分类号： C12M21/02 ,  C12M23/14 ,  C12M23/22 ,  C12M23/34

摘要： The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film.The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof. Therefore, the photobioreactor of the present invention can be easily installed anywhere carbon dioxide is discharged, such as around a power-generating plant, in an urban region, a farm, etc., to culture a variety of photosynthetic organisms, and thus to produce useful substances having economically high added values.

摘要翻译： 光生物反应器技术领域本发明涉及一种光生物反应器，更具体地，涉及一种用于培养利用二氧化碳和光能进行光合作用的微藻等生物体的光生物反应器。 光生物反应器包括：（a）由光合生物发生光合作用的反应容器; （b）形成在反应容器的外部上端的多用途入口/出口; （c）连接到反应容器外部的多功能入口/出口的外管; 和（d）连接到反应容器内部的多功能入口/出口的内管，其中反应容器由透明膜制成。 根据本发明的光生物反应器的优点在于其中发生光合作用的反应容器是板式的并且由透明膜制成，因此实现了改善的透光率和迁移率，并且使得其经济上有利的制造和操作。 因此，本发明的光生物反应器可以容易地安装在诸如在发电厂周围，城市地区，农场等中排放的二氧化碳的任何地方，以培养各种光合生物，从而产生 具有经济高附加值的有用物质。

公开(公告)号：US20100279372A1

公开(公告)日：2010-11-04

申请号：US12499383

申请日：2009-07-08

申请人： Hwa Young CHO ,  Sang Jun SIM ,  Jin Woo KIM ,  Se Jong HAN ,  Jae Chan PARK ,  Sung Min PARK

发明人： Hwa Young CHO ,  Sang Jun SIM ,  Jin Woo KIM ,  Se Jong HAN ,  Jae Chan PARK ,  Sung Min PARK

IPC分类号： C13K1/02 ,  C12P7/02 ,  C12P7/10 ,  C08B1/00

CPC分类号： C12P7/10 ,  C08H8/00 ,  C12P2201/00 ,  C13K1/02 ,  Y02E50/16 ,  Y02E50/343 ,  Y02P30/20

摘要： Disclosed is a method of pretreating lignocellulose-based biomass by extracting lignin from biomass by adding a solvent for dissolving lignin to the lignocellulose-based biomass including lignin, hemicellulose and cellulose, and extracting the cellulose and/or hemicellulose by adding an ionic liquid to the remaining biomass after extracting the lignin.

摘要翻译： 公开了一种通过将木质素溶解于木素纤维素类生物质（包括木质素，半纤维素和纤维素）中并将木质素溶解于木素纤维素的生物质中，通过将离子液体加入到纤维素和/或半纤维素中而从生物质中提取木质素来预处理木质纤维素基生物质的方法 提取木质素后剩余生物量。

公开(公告)号：US20070275371A1

公开(公告)日：2007-11-29

申请号：US11639241

申请日：2006-12-15

申请人： Sang-jun Sim ,  Sang-wook Lee ,  Dong June Ahn ,  Doo ho Yang

发明人： Sang-jun Sim ,  Sang-wook Lee ,  Dong June Ahn ,  Doo ho Yang

IPC分类号： C12Q1/70 ,  C07D207/00 ,  G01N33/53 ,  G01N33/566 ,  G01N33/573 ,  G01N33/554 ,  C07D211/72

CPC分类号： G01N33/54353 ,  G01N33/5432

摘要： There is provided a polydiacetylene supramolecule comprising diacetylene molecules, capable of immobilizing a receptor molecule having a thiol group. Since the polydiacetylene supramolecule has a receptor immobilized thereon having a thiol group, for example, an antibody, and thus shows color transition when reacting with a sample, an antigen can be detected through specific color transition of the polydiacetylene when employing in a receptor-ligand reaction, for example, an antibody-antigen reaction.

摘要翻译： 提供了包含能够固定具有硫醇基的受体分子的二乙炔分子的聚二乙炔超分子。 由于聚二乙炔超分子具有固定在其上的受体，例如具有硫醇基的受体，例如抗体，因此当与样品反应时显示颜色转变，当在受体配体中使用时，可以通过聚二乙炔的特定颜色转变来检测抗原 反应，例如抗体 - 抗原反应。

公开(公告)号：US20080166776A1

公开(公告)日：2008-07-10

申请号：US11817022

申请日：2006-03-16

申请人： Sang-Jun Sim ,  Sang-Woo Park ,  Sung-Tae Yoon

发明人： Sang-Jun Sim ,  Sang-Woo Park ,  Sung-Tae Yoon

IPC分类号： C12P17/16

CPC分类号： C12P17/181

摘要： The present invention relates to a method that improves productivity of a useful secondary metabolite by eliminating biological by-products effectively during the culture of a useful secondary metabolite-producing microorganism, and more particularly, in order to efficiently eliminate biological by-products formed during the culture of Sorangium cellulosum, the present invention relates to a method for producing epothilones comprising that a cation exchange resin is added to said culture medium or said culture broth is recirculated through a column charged with a cation exchange resin. According to the present invention, the effective elimination of by-products formed during the culture of Sorangium cellulosum enhances growth rate of the microorganism as well as dramatically improves productivity of epothilones, a useful secondary metabolite

摘要翻译： 本发明涉及通过在有用的次生代谢产物微生物的培养期间有效地消除生物副产物来提高有用的次级代谢产物的生产率的方法，更具体地说，为了有效地消除在 本发明涉及一种生产埃坡霉素的方法，包括将阳离子交换树脂加入到所述培养基中，或者所述培养液通过装有阳离子交换树脂的柱再循环。 根据本发明，有效消除在丝兰纤维素培养过程中形成的副产物提高了微生物的生长速率，并且显着地提高了埃坡霉素（一种有用的次级代谢物）的生产率

公开(公告)号：US20070196894A1

公开(公告)日：2007-08-23

申请号：US11677302

申请日：2007-02-21

申请人： Sang-jun SIM ,  Chang-deok KANG

发明人： Sang-jun SIM ,  Chang-deok KANG

IPC分类号： C12P23/00 ,  C07C35/18

CPC分类号： C07C403/24 ,  C12P23/00

摘要： There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2). The present invention can not only maximize the efficiency of separating astaxanthin in high concentration by providing a very simple process technique capable of recovering free-astaxanthin selectively from H. pluvialis culture with high efficiency, but also realize the economical and simple operation of the process of separating free-astaxanthin by repeatedly using an alkanic solvent as a mediating solvent for extracting free-astaxanthin. Such a separation method can be used in extracting and separating a carotinoid analogue containing astaxanthin from a useful microalgae culture in addition to Haematococcus species.

摘要翻译： 提供了一种从绿藻中选择性分离游离虾青素的方法，更具体地说，涉及一种从红潮球菌中选择性分离游离虾青素的方法，该方法包括：将含有积雪球菌的细菌培养物与烷烃溶剂混合并搅拌， 从而得到含有游离虾青素和虾青素的虾青素的烷烃溶剂提取物（步骤1）。 并将该烷醇溶剂提取物与醇混合并搅拌，从而得到含有游离虾青素的醇提取物（步骤2）。 本发明不仅可以通过提供一种非常简单的工艺技术来最大限度地提高高浓度虾青素的分离效率，而且能够高效率地从胡萝卜水溶液中选择性地回收游离虾青素，同时也实现了经济，简便的操作过程 通过反复使用烷烃溶剂作为提取游离虾青素的介质溶剂分离游离虾青素。 这种分离方法可以用于从除血球菌属之外的有用的微藻培养物中提取和分离含有虾青素的类胡萝卜素类似物。

公开(公告)号：US20120028267A1

公开(公告)日：2012-02-02

申请号：US13060177

申请日：2009-08-21

申请人： Sang Jun Sim ,  Jun Pyo Kim

发明人： Sang Jun Sim ,  Jun Pyo Kim

IPC分类号： G01N27/00 ,  C12N9/99 ,  C07K14/00 ,  C07H99/00 ,  C07H1/00 ,  C07K1/107 ,  C07K16/00 ,  C07K2/00 ,  B82Y5/00 ,  B82Y15/00

CPC分类号： G01N33/54353 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/174 ,  G01N33/54373 ,  G01N33/54393

摘要： Disclosed is a method of detecting even a very small amount of a target substance by mixing a linker and a spacer at a suitable ratio and immobilizing the mixture on the surface of carbon nanotubes in a carbon nanotube-based biosensor. This method detects a specific substance at the level of femtomoles and lowers the detection limit of conventional carbon nanotube transistor sensors. Accordingly, the method detects even a very small amount of a target substance, and thus the carbon nanotube-based biosensor is a highly useful sensor which can be used either as a medical sensor for diagnosing diseases or as an environmental sensor.

摘要翻译： 公开了通过以适当的比例混合接头和间隔物来均匀检测目标物质的方法，并将该混合物固定在碳纳米管基生物传感器中的碳纳米管表面上。 该方法可以检测飞镖水平的特定物质，降低常规碳纳米管晶体管传感器的检测限。 因此，该方法即使检测到极少量的目标物质，因此基于碳纳米管的生物传感器是可用作诊断疾病的医疗传感器或作为环境传感器的高度有用的传感器。

公开(公告)号：US20110014724A1

公开(公告)日：2011-01-20

申请号：US12867355

申请日：2008-05-26

申请人： Sang Jun Sim ,  Jun Pyo Kim ,  Cuong Cao

发明人： Sang Jun Sim ,  Jun Pyo Kim ,  Cuong Cao

IPC分类号： G01N33/553 ,  B05D1/00 ,  B05D3/10

CPC分类号： G01N33/54346 ,  G01N33/54373

摘要： Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.

摘要翻译： 公开了使用金纳米颗粒的局部表面等离子体共振（LSPR）检测生物产物的方法，其可以在将金纳米颗粒固定在玻璃面板上之后基于由抗原 - 抗体反应发生的最大波长的变化来诊断生物产物。 使用这种方法的传感器具有高灵敏度，价格低廉并且使得快速诊断成为可能，从而适用于与环境污染物，病原体等相关的各种生物领域以及疾病的诊断。 此外，与使用SPR的常规方法相比，它提供了用于制造具有更高灵敏度，低价格和快速性能的传感器的技术。