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    Detectably labeled dual conformation oligonucleotide probes, assays and kits
    1.
    发明授权
    Detectably labeled dual conformation oligonucleotide probes, assays and kits 失效
    可检测标记的双构型寡核苷酸探针，测定和试剂盒

    公开(公告)号：US5925517A

    公开(公告)日：1999-07-20

    申请号：US439819

    申请日：1995-05-12

    申请人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    发明人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6841

    摘要： Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.

    摘要翻译： 用于检测核酸靶序列的单分子和双分子杂交探针包含靶补体序列，在不存在靶序列的情况下将探针保持在封闭构象中的亲和对，以及当探针处于封闭状态时相互作用的标记对 构象，或对于某些单分子探针，非交互式标记。 靶和补体序列的杂交将探针转移到开放构象。 由于标签对的相互作用减少或通过检测来自非交互式标签的信号，该位移是可检测的。 某些单分子探针可以区分不同于一个核苷酸的靶和非靶序列。 此外，通用的茎和试剂盒可用于构建所述探针。 此外，利用所述探针和试剂盒进行这种测定的测定。

    Detectably labeled, dual conformation oligonucleotide probes, assays and kits
    2.
    发明授权
    Detectably labeled, dual conformation oligonucleotide probes, assays and kits 有权
    可检测标记的双构型寡核苷酸探针，测定和试剂盒

    公开(公告)号：US6103476A

    公开(公告)日：2000-08-15

    申请号：US268402

    申请日：1999-03-15

    申请人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    发明人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6841

    摘要： Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.

    摘要翻译： 用于检测核酸靶序列的单分子和双分子杂交探针包含靶补体序列，在不存在靶序列的情况下将探针保持在封闭构象中的亲和对，以及当探针处于封闭状态时相互作用的标记对 构象，或对于某些单分子探针，非交互式标记。 靶和补体序列的杂交将探针转移到开放构象。 由于标签对的相互作用减少或通过检测来自非交互式标签的信号，该位移是可检测的。 某些单分子探针可以区分不同于一个核苷酸的靶和非靶序列。 此外，通用的茎和试剂盒可用于构建所述探针。 此外，利用所述探针和试剂盒进行这种测定的测定。

    Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA
    7.
    发明授权
    Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA 失效
    用于合成可替代RNA的人工基因的靶向依赖性合成

    公开(公告)号：US5112734A

    公开(公告)日：1992-05-12

    申请号：US358399

    申请日：1989-05-26

    申请人： Fred R. Kramer ,  Paul M. Lizardi

    发明人： Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6867 ,  Y10S435/805 ,  Y10S436/815

    摘要： This invention pertains to an improved method for detecting a nucleic acid target sequence with a replicatable RNA reporter system. Two polymerase-mediated reactions are used to generate a target-specific gene containing a DNA sequence for a replicatable RNA. Transcription of the target-specific gene yields a replicatable RNA which is amplified by replication. Synthesis of the gene and the replicatable RNA is strictly dependent upon specific interaction with the target sequence. Consequently, the amplified signal (RNA) is target-dependent and background signal is reduced.

    摘要翻译： 本发明涉及用可复制的RNA报告系统检测核酸靶序列的改进方法。 使用两个聚合酶介导的反应来产生含有可复制RNA的DNA序列的靶特异性基因。 目标特异性基因的转录产生通过复制扩增的可复制的RNA。 基因和可复制的RNA的合成严格依赖于与靶序列的特异性相互作用。 因此，放大信号（RNA）是靶依赖性的，并且背景信号被降低。

    Method of using replicatable hybridzable recombinant RNA probes
    9.
    发明授权
    Method of using replicatable hybridzable recombinant RNA probes 失效
    使用可重复杂交的重组RNA探针的方法

    公开(公告)号：US5503979A

    公开(公告)日：1996-04-02

    申请号：US296866

    申请日：1994-08-26

    申请人： Fred R. Kramer ,  Paul M. Lizardi

    发明人： Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C07H21/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/532

    CPC分类号： G01N33/532 ,  C07H21/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/682 ,  C12Q1/6832 ,  C12Q1/6867 ,  C12Q1/6876 ,  C12Q1/6893 ,  C12Q1/703 ,  Y10S435/948

    摘要： The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heterologus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.

    摘要翻译： 本发明提供可重复和可杂交的重组单链RNA探针分子，其包含：用于结合RNA指导的RNA聚合酶的识别序列; 通过聚合酶引发产物链合成所需的序列; 和插入重组分子的内部区域的特定位点并与感兴趣的寡核苷酸或多核苷酸互补的异源RNA序列。 本发明还提供了用于确定样品中目的寡聚物或多核苷酸的浓度的存在以及用于同时测定样品中几种不同的目标的寡核苷酸或多核苷酸的存在或浓度的方法。