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    Detectably labeled dual conformation oligonucleotide probes, assays and kits
    3.
    发明授权
    Detectably labeled dual conformation oligonucleotide probes, assays and kits 失效
    可检测标记的双构型寡核苷酸探针，测定和试剂盒

    公开(公告)号：US5925517A

    公开(公告)日：1999-07-20

    申请号：US439819

    申请日：1995-05-12

    申请人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    发明人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6841

    摘要： Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.

    摘要翻译： 用于检测核酸靶序列的单分子和双分子杂交探针包含靶补体序列，在不存在靶序列的情况下将探针保持在封闭构象中的亲和对，以及当探针处于封闭状态时相互作用的标记对 构象，或对于某些单分子探针，非交互式标记。 靶和补体序列的杂交将探针转移到开放构象。 由于标签对的相互作用减少或通过检测来自非交互式标签的信号，该位移是可检测的。 某些单分子探针可以区分不同于一个核苷酸的靶和非靶序列。 此外，通用的茎和试剂盒可用于构建所述探针。 此外，利用所述探针和试剂盒进行这种测定的测定。

    Detectably labeled, dual conformation oligonucleotide probes, assays and kits
    4.
    发明授权
    Detectably labeled, dual conformation oligonucleotide probes, assays and kits 有权
    可检测标记的双构型寡核苷酸探针，测定和试剂盒

    公开(公告)号：US6103476A

    公开(公告)日：2000-08-15

    申请号：US268402

    申请日：1999-03-15

    申请人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    发明人： Sanjay Tyagi ,  Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6841

    摘要： Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.

    摘要翻译： 用于检测核酸靶序列的单分子和双分子杂交探针包含靶补体序列，在不存在靶序列的情况下将探针保持在封闭构象中的亲和对，以及当探针处于封闭状态时相互作用的标记对 构象，或对于某些单分子探针，非交互式标记。 靶和补体序列的杂交将探针转移到开放构象。 由于标签对的相互作用减少或通过检测来自非交互式标签的信号，该位移是可检测的。 某些单分子探针可以区分不同于一个核苷酸的靶和非靶序列。 此外，通用的茎和试剂盒可用于构建所述探针。 此外，利用所述探针和试剂盒进行这种测定的测定。

    Fixed address analysis of sequence tags
    7.
    发明授权
    Fixed address analysis of sequence tags 失效
    序列标签的固定地址分析

    公开(公告)号：US06677121B2

    公开(公告)日：2004-01-13

    申请号：US09855793

    申请日：2001-05-15

    申请人： Paul M. Lizardi ,  Matthew E. Roth ,  Li Feng ,  Cesar E. Guerra ,  Shane C. Weber ,  Joseph C. Kaufman ,  Darin R. Latimer

    发明人： Paul M. Lizardi ,  Matthew E. Roth ,  Li Feng ,  Cesar E. Guerra ,  Shane C. Weber ,  Joseph C. Kaufman ,  Darin R. Latimer

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6855 ,  C12Q1/6809 ,  C12Q1/6837 ,  Y10S977/924 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/313 ,  C12Q2565/513

    摘要： Disclosed is a method for the comprehensive analysis of nucleic acid samples and a detector composition for use in the method. The method, referred to as Fixed Address Analysis of Sequence Tags (FAAST), involves generation of a set of nucleic acid fragments having a variety of sticky end sequences; indexing of the fragments into sets based on the sequence of sticky ends; associating a detector sequence with the fragments; sequence-based capture of the indexed fragments on a detector array; and detection of the fragment labels. Generation of the multiple sticky end sequences is accomplished by incubating the nucleic acid sample with one or more nucleic acid cleaving reagents. The indexed fragments are captured by hybridization and coupling, preferably by ligation, to a probe. The method allows a complex sample of nucleic acid to be quickly and easily cataloged in a reproducible and sequence-specific manner. One form of the method allows determination of associations, in a nucleic acid molecule, of different combinations of known or potential sequences. Another form of the method assesses modification of sequences in nucleic acid molecules by basing cleavage of the molecules on the presence or absence of modification.

    摘要翻译： 公开了用于综合分析核酸样品的方法和用于该方法的检测器组合物。 称为序​​列标签的固定地址分析（FAAST）的方法涉及产生具有各种粘性末端序列的一组核酸片段; 基于粘性末端的顺序将片段索引到集合中; 将检测器序列与片段相关联; 检测器阵列上索引片段的基于序列的捕获; 并检测片段标签。 通过将核酸样品与一种或多种核酸切割试剂孵育来实现多粘性末端序列的产生。 索引的片段通过杂交和偶联，优选通过连接被捕获到探针。 该方法允许复制的核酸样品以可再现和序列特异性方式快速且容易地编目。 该方法的一种形式允许确定核酸分子中已知或潜在序列的不同组合的缔合。 该方法的另一种形式通过在存在或不存在修饰的基础上分解裂解来评估核酸分子中序列的修饰。

    Fixed address analysis of sequence tags
    8.
    发明授权
    Fixed address analysis of sequence tags 失效
    序列标签的固定地址分析

    公开(公告)号：US06261782B1

    公开(公告)日：2001-07-17

    申请号：US09544713

    申请日：2000-04-06

    申请人： Paul M. Lizardi ,  Matthew E. Roth ,  Li Feng ,  Cesar E. Guerra ,  Shane C. Weber ,  Joseph C. Kaufman ,  Darin R. Latimer

    发明人： Paul M. Lizardi ,  Matthew E. Roth ,  Li Feng ,  Cesar E. Guerra ,  Shane C. Weber ,  Joseph C. Kaufman ,  Darin R. Latimer

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6855 ,  C12Q1/6809 ,  C12Q1/6837 ,  Y10S977/924 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/313 ,  C12Q2565/513

    摘要： Disclosed is a method for the comprehensive analysis of nucleic acid samples and a detector composition for use in the method. The method, referred to as Fixed Address Analysis of Sequence Tags (FAAST), involves generation of a set of nucleic acid fragments having a variety of sticky end sequences; indexing of the fragments into sets based on the sequence of sticky ends; associating a detector sequence with the fragments; sequence-based capture of the indexed fragments on a detector array; and detection of the fragment labels. Generation of the multiple sticky end sequences is accomplished by incubating the nucleic acid sample with one or more nucleic acid cleaving reagents. The indexed fragments are captured by hybridization and coupling, preferably by ligation, to a probe. The method allows a complex sample of nucleic acid to be quickly and easily cataloged in a reproducible and sequence-specific manner. One form of the method allows determination of associations, in a nucleic acid molecule, of different combinations of known or potential sequences. Another form of the method assesses modification of sequences in nucleic acid molecules by basing cleavage of the molecules on the presence or absence of modification.

    摘要翻译： 公开了用于综合分析核酸样品的方法和用于该方法的检测器组合物。 称为序​​列标签的固定地址分析（FAAST）的方法涉及产生具有各种粘性末端序列的一组核酸片段; 基于粘性末端的序列将片段索引到集合中; 将检测器序列与片段相关联; 检测器阵列上索引片段的基于序列的捕获; 并检测片段标签。 通过将核酸样品与一种或多种核酸切割试剂孵育来实现多粘性末端序列的产生。 索引的片段通过杂交和偶联，优选通过连接被捕获到探针。 该方法允许复制的核酸样品以可再现和序列特异性方式快速且容易地编目。 该方法的一种形式允许确定核酸分子中已知或潜在序列的不同组合的缔合。 该方法的另一种形式通过在存在或不存在修饰的基础上分解裂解来评估核酸分子中序列的修饰。

    Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA
    9.
    发明授权
    Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA 失效
    用于合成可替代RNA的人工基因的靶向依赖性合成

    公开(公告)号：US5112734A

    公开(公告)日：1992-05-12

    申请号：US358399

    申请日：1989-05-26

    申请人： Fred R. Kramer ,  Paul M. Lizardi

    发明人： Fred R. Kramer ,  Paul M. Lizardi

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6867 ,  Y10S435/805 ,  Y10S436/815

    摘要： This invention pertains to an improved method for detecting a nucleic acid target sequence with a replicatable RNA reporter system. Two polymerase-mediated reactions are used to generate a target-specific gene containing a DNA sequence for a replicatable RNA. Transcription of the target-specific gene yields a replicatable RNA which is amplified by replication. Synthesis of the gene and the replicatable RNA is strictly dependent upon specific interaction with the target sequence. Consequently, the amplified signal (RNA) is target-dependent and background signal is reduced.

    摘要翻译： 本发明涉及用可复制的RNA报告系统检测核酸靶序列的改进方法。 使用两个聚合酶介导的反应来产生含有可复制RNA的DNA序列的靶特异性基因。 目标特异性基因的转录产生通过复制扩增的可复制的RNA。 基因和可复制的RNA的合成严格依赖于与靶序列的特异性相互作用。 因此，放大信号（RNA）是靶依赖性的，并且背景信号被降低。