公开(公告)号：US20180246057A1

公开(公告)日：2018-08-30

申请号：US15886608

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Thomas Young CHANG ,  David S. KUO ,  Kim Yang LEE ,  Koichi WAGO

IPC: G01N27/447 ,  C12Q1/6874 ,  G01N33/487 ,  B01L3/00

Abstract: A DNA sequencing device, and related method, which include an electrode and a plurality of spaced apart alignment structures. The electrode defines an electrode gap, the electrode being operable to detect a change in tunneling current as a DNA strand passes through the electrode gap. The plurality of spaced apart alignment structures are arranged to position nucleotides of the DNA strand in a predetermined orientation as the DNA strand passes through the electrode gap.

公开(公告)号：US20200371064A1

公开(公告)日：2020-11-26

申请号：US16940486

申请日：2020-07-28

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Thomas Young CHANG ,  David S. KUO ,  Kim Yang LEE ,  Koichi WAGO

IPC: G01N27/447 ,  B01L3/00 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

Abstract: A DNA sequencing device, and related method, which include an electrode and a plurality of spaced apart alignment structures. The electrode defines an electrode gap, the electrode being operable to detect a change in tunneling current as a DNA strand passes through the electrode gap. The plurality of spaced apart alignment structures are arranged to position nucleotides of the DNA strand in a predetermined orientation as the DNA strand passes through the electrode gap.

公开(公告)号：US20200240947A1

公开(公告)日：2020-07-30

申请号：US16845565

申请日：2020-04-10

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： ShuaiGang XIAO ,  David S. KUO ,  Xiaomin YANG ,  Kim Yang LEE ,  Yautzong HSU ,  Michael R. FELDBAUM

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487

Abstract: A DNA sequencing device and methods of making. The device includes a pair of electrodes having a spacing of no greater than about 2 nm, the electrodes being exposed within a nanopore to measure a DNA strand passing through the nanopore. The device can be made by depositing a conductive layer over a sacrificial channel and then removing the sacrificial channel to form the electrode gap.

公开(公告)号：US20230340684A1

公开(公告)日：2023-10-26

申请号：US18305973

申请日：2023-04-24

Applicant: Seagate Technology LLC

Inventor： Thomas Young CHANG ,  Kim Yang LEE ,  Mark T. KIEF ,  Riyan Alex MENDONSA ,  Brett R. HERDENDORF

IPC: C25D1/00 ,  B33Y10/00

CPC classification number: C25D1/003 ,  B33Y10/00

Abstract: A method for depositing a material from a solution to a surface is provided. The method includes depositing, through a deposition channel of a material depositor, the solution in a rich state to the surface, wherein the solution in the rich state includes an initial concentration of the material, onto a surface, applying a predefined electrical output, by the material depositor, through the deposited solution to adhere the material to the surface, and to yield the solution in a depleted state wherein the solution in the depleted state contains a different concentration of the material from the rich solution, and removing, through a removal channel in the material depositor, the deposited solution in the depleted state. The material depositor includes a hydrophilic region defined by a hydrophilic surface through which the material depositor conducts the depositing and the removing and a hydrophobic barrier circumscribing the hydrophilic region.

公开(公告)号：US20180259475A1

公开(公告)日：2018-09-13

申请号：US15886723

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： ShuaiGang XIAO ,  David S. KUO ,  Xiaomin YANG ,  Kim Yang LEE ,  Yautzong HSU ,  Michael R. FELDBAUM

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y5/00

CPC classification number: G01N27/3278 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A DNA sequencing device, and related method, which include a nanopore having a maximum width dimension of no greater than about 50 nm, and a pair of electrodes having a spacing of no greater than about 2 nm, the electrodes being exposed within the nanopore to measure a DNA strand passing through the nanopore.

公开(公告)号：US20180223354A1

公开(公告)日：2018-08-09

申请号：US15886483

申请日：2018-02-01

Applicant: Seagate Technology LLC

Inventor： Thomas Young CHANG ,  Kim Yang LEE ,  David S. KUO ,  Erik J. HUTCHINSON

IPC: C12Q1/6869 ,  G01N33/487 ,  B01L3/00 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0896 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Apparatus and methods to a DNA sequencing device and related methods that includes a substrate, a nanochannel formed in the substrate, a first electrode, a second electrode arranged opposite the first electrode, a distance between the first and second electrodes defining an electrode gap that is exposed within the nanochannel, and at least one actuator operable to move at least one of the first and second electrodes to adjust a size of the electrode gap.

公开(公告)号：US20200249195A1

公开(公告)日：2020-08-06

申请号：US16854607

申请日：2020-04-21

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： David S. KUO ,  Xiaomin YANG ,  ShuaiGang XIAO ,  Kim Yang LEE ,  Koichi R. WAGO ,  Thomas Young CHANG

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  B01L3/00 ,  B81C1/00

Abstract: A DNA sequencing device, and related methods, include a nanopore or nanochannel structure, and a nanoelectrode. The nanoelectrode includes electrode members having free ends exposed within the nanopore or nanochannel structure, an electrode gap defined between of the free ends, and plated portions formed on the free ends to provide a reduced sized for the electrode gap.

公开(公告)号：US20180258480A1

公开(公告)日：2018-09-13

申请号：US15886736

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Thomas Young CHANG ,  Philip L. STEINER ,  Kim Yang LEE ,  David S. KUO

IPC: C12Q1/6869 ,  C40B40/06

Abstract: Apparatus and methods to identify nucleotides of a DNA strand. The method includes exposing the DNA strand to a first dye or peptide, attaching the first dye or peptide to a first type of nucleotide (A,T,C,G) of the DNA strand, the first dye or peptide changing a conductance of the first type of nucleotide to which the first dye or peptide is attached, and measuring a tunneling current signal for all nucleotides of the DNA strand, the changed conductance of the first type of nucleotide providing amplified tunneling current discrimination of the nucleotides of the DNA strand.

公开(公告)号：US20180217083A1

公开(公告)日：2018-08-02

申请号：US15886581

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： David S. KUO ,  Xiaomin YANG ,  ShuaiGang XIAO ,  Kim Yang LEE ,  Koichi WAGO ,  Thomas Young CHANG

IPC: G01N27/327 ,  B01L3/00 ,  B81C1/00

CPC classification number: G01N27/3278 ,  B01L3/502707 ,  B01L3/502715 ,  B81C1/00547 ,  B82Y5/00 ,  C12Q1/6809 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

Abstract: A DNA sequencing device, and related methods, include a nanopore or nanochannel structure, and a nanoelectrode. The nanoelectrode includes electrode members having free ends exposed within the nanopore or nanochannel structure, an electrode gap defined between of the free ends, and plated portions formed on the free ends to provide a reduced sized for the electrode gap.

公开(公告)号：US20180216169A1

公开(公告)日：2018-08-02

申请号：US15886442

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Koichi WAGO ,  ShuaiGang XIAO ,  Xiaomin YANG ,  Kim Yang LEE ,  David S. KUO ,  Thomas Young CHANG

IPC: C12Q1/6825 ,  C12Q1/6874 ,  G01N27/447 ,  G01N33/487 ,  C12Q1/6837 ,  G01N27/414 ,  C12Q1/6806 ,  B01L3/00

CPC classification number: C12Q1/6825 ,  B01L3/502761 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: Apparatus and methods to sequence DNA. A DNA sequencing device includes a passage, a first electrode, and a second electrode. The passage has a width and a length. The first and second electrodes are exposed within the passage and spaced apart from each other to form an electrode gap. The electrode gap is no greater than about 2 nm. The DNA sequencing device is operable to measure with the first and second electrodes a change in electronic signal in response to nucleotides of a DNA strand passing through the electrode gap.