摘要:
An automatic sample pretreatment device is provided which is capable of conducting a series of treatments or reactions from pretreatment to measurement automatically in a short time by employing a separate reaction system from the reaction system for measurement. The automatic sample pretreatment device comprises a sample rack, a pretreatment vessel and a measurement vessel, a liquid dispenser, a first vessel-transfer apparatus having a vessel holder for holding and transferring the vessels, a pretreatment vessel feeding apparatus for feeding the pretreatment vessel to the vessel holder of the first vessel transfer apparatus, a measurement vessel feeding apparatus for feeding the measurement vessel to the vessel holder of the first vessel-transfer apparatus, a vessel discarding hole for discarding the vessel after use, a second vessel-transfer device for transferring the used vessel held at the vessel holder of the first vessel-transfer apparatus to the vessel discarding hole, an incubator equipped with a measurement device for measuring a sample, and a third vessel-transfer device for transferring the vessel held by the vessel holder of the first vessel-transfer apparatus to the incubator.
摘要:
A method of rate calculation in which, in connection with a reaction in which the substrate of an enzyme undergoes the action of the enzyme to be converted to a reaction product, a signal correlated to the concentration of the reaction product is measured at different points of time, those measured values obtained which exceed the upper limit preset in accordance with the detection limit of a detector to be used are excluded while selecting those measured values obtained at different points of time at least equal in number to the independent parameters of the approximating function and which are not greater than said upper limit and the measured values thus selected are weighted by a finite value to effect approximation by said function, provided that said finite value for weighting at least a portion of the measured values are changed continuously in accordance with the magnitudes of said specific measured values. This method is capable of calculating the rate of enzyme activity to accomplish precise quantification of a substance without impairing the continuity of a calibration curve even if measurements are conducted at discontinuous points of time over a measurement range where the rate will change under the influence of the concentrations of the substrate and the reaction product in the reaction mixture.
摘要:
An object of the invention is to predict energy consumptions of a vehicle, using geographic characteristic values which are independent from particular driving patterns and vehicle parameters and unique to respective links. A navigation server predicts energies which are consumed when a vehicle runs on links. The navigation server calculates geographic characteristic values of respective links, the geography of the each link affecting the consumption energy with the geographic characteristic values, the calculation being based on energy consumptions collected from probe vehicles, and calculates predicted energy consumption of each link selected as a processing target, based on the geographic characteristic values. A navigation terminal obtains these predicted energy consumptions and performs route search with the obtained predicted energy consumptions as costs.
摘要:
It is an object of the present invention to provide a route searching which can reflect know-how such as easiness of running included in the probe car data in an entire route from departure place to destination.A center device detects main branch nodes from probe car data received from an in-vehicle terminal device by means of a main branch node detecting section. A probe car data dividing section divides the probe car data into probe segments by the main branch nodes. A route dividing section divides an initial route generated by a initial route generating section based on specified departure place and destination, and a derived route generating section substitutes probe segments for the divided routes so as to generate derived routes. The route selecting section scores the derived routes and select one so as to provide a recommended route in which know-how such as easiness of running included in the probe car data is reflected.
摘要:
There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.
摘要:
It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein/L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.
摘要翻译:本发明的目的是提供具有高内切葡聚糖酶活性并且即使在碱性条件下也表现出高活性的酶,以及编码该酶的基因。 根据本发明的酶具有以下性质:a)显示内切葡聚糖酶活性; 和b)能够以1mg蛋白质/ L或以下的浓度从再生纤维素织物中完全除去绒毛。 具有内切葡聚糖酶活性的本发明的酶是包含如SEQ ID NO:1,3,5,7,9或11所示的氨基酸序列的蛋白质; 其表现出内切葡聚糖酶活性的修饰蛋白质; 或蛋白质或修饰蛋白质的同系物。
摘要:
An object of the invention is to predict energy consumptions of a vehicle, using geographic characteristic values which are independent from particular driving patterns and vehicle parameters and unique to respective links. A navigation server predicts energies which are consumed when a vehicle runs on links. The navigation server calculates geographic characteristic values of respective links, the geography of the each link affecting the consumption energy with the geographic characteristic values, the calculation being based on energy consumptions collected from probe vehicles, and calculates predicted energy consumption of each link selected as a processing target, based on the geographic characteristic values. A navigation terminal obtains these predicted energy consumptions and performs route search with the obtained predicted energy consumptions as costs.
摘要:
A car navigation system solves the problem that the emission test cycle fuel consumption rate indicates the energy consumption per unit distance covered following a prescribed emission test cycle procedure, and therefore, cannot produce a highly accurate prediction value reflecting the landform and the traffics along a route. So, the test cycle characteristic values independent of the vehicle model for vehicle energy consumption in a specified running condition are calculated, and the vehicle energy consumption parameter of the engine or the motor is estimated from the basic vehicle specifications such as the vehicle weight, the power supply type, the emission test cycle fuel consumption rate, the test cycle characteristic values in the emission test cycle and the vehicle characteristics. Using the basic vehicle specifications and the energy consumption parameter, the energy consumption is predicted taking the landform and traffics into consideration.
摘要:
There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.
摘要:
A highly active cellulase suitable for use in a removal of nap of cellulose-containing fibers, a process for reducing cellulose-containing fibers and a process for decoloring denim-dyed cellulose-containing fibers, and its gene are provided. A novel cellulase NCE4 isolated from Humicola insolens is a highly active cellulase, and can be used for various treatments of cellulose-containing fibers.