摘要:
An apparatus for operating a syringe has developed comprising: an upper semi-cylindrical tube section, semi-circular shaped locking groove and lower semi-cylindrical tube section. The upper semi-cylindrical tube section is integrally formed an upper head part with a threaded hole for axial movement. The lower semi-cylindrical tube section forms a top portion, intermediate portion and end portion. A diameter (Dc) at the intermediate portion larger than the diameter (Dtip) at end portion is gradually reduced from intermediate to end portion. The length of the tapered portion is same as that portion of the constant diameter. A tip portion has formed an opened semi-circular shape to simply overlap the syringe over the cartridge for easily snapping-in and out. The syringe piston moves downward with no reverse action when the piston press-unit is rotated, thus it is possible to inject the precise amount of medicine to a patient with constant pressure.
摘要:
An apparatus for operating a syringe piston is developed, which is comprised of: a syringe mounting section for fixedly holding a syringe, a thread hole formed at an upper head part of the syringe mounting section, and a piston press unit having a threaded rod, which is inserted into the thread hole so that the syringe piston press unit can move up and down along the thread hole, a locking slot for fixedly inserting and retaining the medicine filled syringe, a semi-cylindrical tube to assemble with the syringe mounting section. The syringe filled with medical cement is attached to the syringe mounting section and the syringe piston is moved downward with no reverse movement when the piston press unit is rotated, so the medical cement contained in the syringe is injected with a constant pressure, so that a predetermined amount of medical cement is precisely injected into a patient's body.
摘要:
A compound for bone forming and a method for producing the same are provided using a mixture of an osteoblast and a bio-matrix. The method comprises: isolating osteoblasts from bone tissue and culturing the isolated osteoblasts to prepare an osteoblast suspension; and mixing the resulting osteoblast suspension with a bio-matrix to prepare an osteoblast therapeutic agent. A bone formation method is provided that results in no clinical graft rejection, and is capable of achieving effective and rapid bone formation via injection of a compound which has been pre-shaped to a certain extent, so as to alleviate problems associated with bone tissue formation in unwanted regions resulting from escape of injected osteoblasts from the targeted site for bone formation and then propagation thereof to other sites via the blood stream, which are caused by injection of an osteoblast suspension.
摘要:
The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bone marrow; and mixing the nucleated cells with a maintenance buffer for bone formation. According to the invention, bone marrow-derived nucleated cells, which can be grafted into a site in need of bone formation or bone defect treatment, can be separated in a rapid and convenient manner. As a result, bone formation in emergency patients or patients, who require repeated surgical operations, can be effectively achieved by separating the nucleated cells for bone formation in surgical locations in a convenient and rapid manner and injecting the separated cells into the patients.
摘要:
A method for producing the fibrin contained semi-solid osteoblast composition has developed for treating the bone fracture. The method is comprised of: isolating osteoblasts from a bone tissue and culturing/proliferating the isolated osteoblasts in Dulbecco's Modified Eagle's Medium (DMEM) or Minimum Essential Medium, Alpha Modification (α-MEM) to prepare an osteoblast suspension. Then, mixing the resulting osteoblast suspension with a coagulation factor to prepare an osteoblast therapeutic agent. Accordingly, the fibrin contained semi-solid osteoblast composition of the present invention has capability to achieve the bone grafting for performing bone union without the clinical graft rejection. It is possible to uniformly distribute the fibrin mixed semi-solid osteoblast composition into the affected area of the bone fracture via injection. Therefore, the fibrin contained semi-solid osteoblast composition is effectively and rapidly cure the bone fracture via constant injection of into the affected area.
摘要:
A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg/mL.
摘要:
A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg/mL.
摘要:
A method is provided for utilizing a cell therapy product facility and an internet franchise market business comprising: producing a cell therapy product by a CT (Cell Therapy)-module (1) of prefabricated units having specific functions, and; storing of hematopoietic stem cells, bone marrow cells and other cells, by a BC (Banking of Cell and Tissue)-module (2) of prefabricated units having specific functions. Both modules eliminate contamination and maximize efficiency. It provides an operating method and a use method and business method for the above cell therapy product facility. The invention enables easy, low cost production of a product having sufficient quality to be transplanted into patients and put into clinical application within a short period of time. The facility module of the invention can be installed and used wherever adequate space is available. The invention provides improved quality and reliability of cell therapy products.
摘要翻译:提供了一种利用细胞疗法产品设备和网络营销市场业务的方法,包括:通过具有特定功能的预制单元的CT(Cell Therapy)模块(1)产生细胞治疗产品; 通过具有特定功能的预制单元的BC(Cell of Cell and Tissue)模块(2)存储造血干细胞,骨髓细胞和其它细胞。 两个模块都能消除污染并最大限度地提高效率。 它提供了上述细胞治疗产品设施的操作方法和使用方法和商业方法。 本发明能够容易,低成本地生产具有足够质量的产品移植到患者中并在短时间内投入临床应用。 本发明的设施模块可以在有足够空间的地方安装和使用。 本发明提供了改善细胞治疗产品的质量和可靠性。
摘要:
A cartilage therapeutic composition is developed for clinical transplantation into articulatio genu (knee joints) or ankle joints. It has clinical significance for symptomatic cartilage defects of the femoral condyle (medial, lateral, or trochlear) and bone cartilage defects of the talus (anklebone) in human or animal hosts, The cartilage therapeutic composition comprises a mixture of components of chondrocytes isolated and expanded or differentiated from a host such as a human or animal, and thrombin and a fibrinogen matrix containing fibrinogen. An application of the cartilage therapeutic composition is that a mixture of thrombin, chondrocyte components and a fibrinogen matrix is injected into a cartilage defect region followed by solidification therein. It provides rapid healing and effective regeneration of cartilage without surgical operation. It has the merits of safety and simplicity by allowing the use of an arthroscope for transplantation.
摘要:
A method of transplanting an injectable chondrocyte is developed for autologous chondrocyte transplantation, comprising mixing matrices including fibrin, hyaluronic acid and collagen, which are the main ingredients of animal cartilage, and injecting the resulting mixture into a damaged cartilage region. One 1 ml syringe is prepared by mixing 1 ml of a chondrocyte culture with white or light-yellow lyophilized powder (fibrinogen). A second 1 ml syringe is prepared by mixing 1 ml of chondrocyte culture with white lyophilized powder (thrombin), adding 0.1 cc of that mixture to the entire contents of two vials containing chondrocyte suspension, and mixing well. The two syringes are then mounted on a syringe stand, and a mixing tip is mounted to their tips. The contents are mixed in the mixing tip while injecting the resulting mixture into a damaged cartilage region of an animal.