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    DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
    3.
    发明授权
    DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs 失效
    编码新融合蛋白的DNA和通过DNA表达制备有用多肽的方法

    公开(公告)号：US06506595B2

    公开(公告)日：2003-01-14

    申请号：US09280030

    申请日：1999-03-26

    申请人： Seiji Sato ,  Naohiko Higashikuni ,  Toshiyuki Kudo ,  Masaaki Kondo

    发明人： Seiji Sato ,  Naohiko Higashikuni ,  Toshiyuki Kudo ,  Masaaki Kondo

    IPC分类号： C12N1500

    CPC分类号： C12N15/625 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K14/655 ,  C07K2319/034 ,  C07K2319/21 ,  C07K2319/50 ,  C07K2319/75

    摘要： This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, the sequences being linked in order, the signal peptide, tag and linker being optional sequences; and wherein the nucleotide sequence encoding a fusion protein is ligated to 3′-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.

    摘要翻译： 本发明涉及包含编码融合蛋白的核苷酸序列的DNA，其中融合蛋白包含：用于芽孢杆菌细胞壁蛋白（CWP）的信号肽序列; 用于分离和纯化融合蛋白的标签序列; 接头序列; 用于化学或酶切割的序列; 和外源多肽序列，序列依次连接，信号肽，标签和接头是任选的序列; 并且其中将编码融合蛋白的核苷酸序列连接到包含芽孢杆菌启动子区的核酸序列的3'-末端; 涉及包含该DNA的载体; 涉及属于包含载体的芽孢杆菌属的细菌; 以及通过细菌培养制备有用多肽的方法。

    Sexing method of bovine embyros
    5.
    发明授权
    Sexing method of bovine embyros 失效
    牛的embyros的性别的方法

    公开(公告)号：US5461145A

    公开(公告)日：1995-10-24

    申请号：US984044

    申请日：1992-12-02

    申请人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    发明人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6879

    摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

    摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

    Sexing method of bovine embryos
    6.
    发明授权
    Sexing method of bovine embryos 失效
    牛胚胎的性别方法

    公开(公告)号：US5661011A

    公开(公告)日：1997-08-26

    申请号：US458393

    申请日：1995-06-02

    申请人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    发明人： Toshiyuki Kudo ,  Yoshiaki Itagaki ,  Seiji Sato ,  Shizuyo Sutou ,  Toyoo Nakamura

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6879

    摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

    摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

    CLEANING APPARATUS FOR A PROBE
    8.
    发明申请
    CLEANING APPARATUS FOR A PROBE 审中-公开
    清洁设备探针

    公开(公告)号：US20080280542A1

    公开(公告)日：2008-11-13

    申请号：US12115495

    申请日：2008-05-05

    申请人： Yuji Miyagi ,  Tetsuya Iwabuchi ,  Toshiyuki Kudo

    发明人： Yuji Miyagi ,  Tetsuya Iwabuchi ,  Toshiyuki Kudo

    IPC分类号： B24B7/00

    CPC分类号： B24B19/16 ,  B24B19/226

    摘要： The present invention provides a cleaning apparatus capable of removing foreign matters attached to a tip of a probe effectively without impairing the durability of the probe. The cleaning apparatus for the probe comprises a base plate having a rough surface and a surface layer formed to conform to and cover the rough surface for the purpose of providing a polishing surface for the probe and having lower hardness than hardness of the probe tip of the probe.

    摘要翻译： 本发明提供一种清洁装置，其能够有效地除去附着于探针的尖端的异物，而不会损害探针的耐久性。 用于探针的清洁装置包括具有粗糙表面的基板和形成为符合并覆盖粗糙表面的表面层，以提供用于探针的抛光表面，并且具有比硬度更高的探针尖端的硬度 探测。

    Electronic contract safekeeping method, electronic contract verifying method, contractor server, contract safekeeping server, electronic contract safekeeping system, and memory medium
    9.
    发明授权
    Electronic contract safekeeping method, electronic contract verifying method, contractor server, contract safekeeping server, electronic contract safekeeping system, and memory medium 失效
    电子合同保管方式，电子合同验证方法，承包商服务器，合同保管服务器，电子合同保管系统和存储介质

    公开(公告)号：US06952686B1

    公开(公告)日：2005-10-04

    申请号：US09926148

    申请日：2000-09-25

    申请人： Toshiyuki Kudo

    发明人： Toshiyuki Kudo

    IPC分类号： G06Q30/00 ,  G21C17/60

    CPC分类号： G06Q30/06 ,  G06Q20/401 ,  G06Q50/188

    摘要： An electronic safekeeping method, including displaying a contractual coverage of a contract at a covenantee terminal connected to a contractor server storing information of the contractual coverage; inputting contract agreement information representing whether agreeing to the contractual coverage or not, at the covenantee terminal; inputting safekeeping agreement information representing whether agreeing to a safekeeping of the contractual coverage or not, at the covenantee terminal, when the contract agreement information agreeing to the contractual coverage is inputted; requesting a contract safekeeping server to store the contractual coverage, when the safekeeping agreement information agreeing to the safekeeping is inputted; issuing a contract ID for identifying the contract at the contract safekeeping server in accordance with the request; and transferring the issued contract ID from the contract safekeeping server to the covenantee terminal and the contractor server.

    摘要翻译： 一种电子保管方法，包括在与承包商服务器连接的公约终端上显示合同的合同范围，存储合同范围的信息; 在契约方终端输入代表是否同意合同范围的合同协议信息; 在合同协议信息同意输入合同协议信息时，在契约终端输入代表是否同意保管合同范围的保管协议信息; 当输入同意保管的保管协议信息时，请求合同保管服务器存储合同范围; 根据要求，在合同保管服务器上签发合同识别合同; 并将已签发的合同编号从合同保管服务器转移给契约终端和承包商服务器。

    Method and apparatus for testing integrated circuit
    10.
    发明授权
    Method and apparatus for testing integrated circuit 有权
    集成电路测试方法和装置

    公开(公告)号：US08680880B2

    公开(公告)日：2014-03-25

    申请号：US12637656

    申请日：2009-12-14

    申请人： Hidehiro Kiyofuji ,  Tetsuya Iwabuchi ,  Toshiyuki Kudo ,  Seiji Kanazawa

    发明人： Hidehiro Kiyofuji ,  Tetsuya Iwabuchi ,  Toshiyuki Kudo ,  Seiji Kanazawa

    IPC分类号： G01R31/20

    CPC分类号： G01R31/2875

    摘要： An embodiment of a method for testing an integrated circuit comprises a first step for determining at least one of a group selected from whether or not the chuck top receiving the integrated circuit exists near a probe card which transmits and receives electrical signals to and from the integrated circuit, whether or not the integrated circuit is under testing, and whether or not the probe card has a given temperature, and a second step for adjusting power for heating to be supplied to a heating element provided in the probe card according to the determination result in the first step.

    摘要翻译： 用于测试集成电路的方法的一个实施例包括第一步骤，用于确定选自接收集成电路的卡盘顶部是否存在于探针卡附近的组中的至少一个，所述探针卡向集成电路发送和接收电信号 电路，集成电路是否在测试中，以及探针卡是否具有给定的温度，以及第二步骤，用于根据确定结果调节供应到设置在探针卡中的加热元件的加热功率 在第一步。