公开(公告)号：US08796235B2

公开(公告)日：2014-08-05

申请号：US10544146

申请日：2004-02-23

申请人： Shyam S. Mohapatra ,  Weidong Zhang

发明人： Shyam S. Mohapatra ,  Weidong Zhang

IPC分类号： C12N15/11 ,  C12N15/00 ,  C12N15/86 ,  C12N7/00 ,  A61K39/12

CPC分类号： C12N15/111 ,  A61K48/005 ,  A61K48/0058 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2750/14143

摘要： The present invention pertains to vectors for regulating gene expression having at least one gene expressing cassette and at least one gene suppressing cassette, wherein the gene expression cassette encodes a polypeptide of interest, and wherein the gene suppressing cassette encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference. The present invention further includes vectors that contain suppressor cassettes in conjunction with cassettes upregulating gene expression regulated by either a constitutive promoter, such as a general CMV promoter, or a tissue specific promoter. The present invention further includes vectors that contain Dengue virus gene suppression cassettes. The present invention further includes pharmaceutical compositions containing such vectors, methods of modulating the expression of genes in a host using such vectors, and method of producing such vectors.

摘要翻译： 本发明涉及用于调节基因表达的载体，所述载体具有至少一个表达基因的盒和至少一个基因抑制盒，其中所述基因表达盒编码目的多肽，并且其中所述基因抑制盒编码短干扰RNA（siRNA） 通过RNA干扰降低靶基因表达的分子。 本发明还包括载体，其含有与上调由组成型启动子如一般CMV启动子或组织特异性启动子调节的基因表达的盒结合的抑制盒。 本发明还包括含有登革热病毒基因抑制盒的载体。 本发明还包括含有这些载体的药物组合物，使用这种载体调节宿主中基因表达的方法，以及生产这种载体的方法。

公开(公告)号：US20180195037A1

公开(公告)日：2018-07-12

申请号：US15490100

申请日：2017-04-18

申请人： Subhra Mohapatra ,  Shyam S. Mohapatra ,  Yvonne Kathleen Davis ,  Chunyan Wang

发明人： Subhra Mohapatra ,  Shyam S. Mohapatra ,  Yvonne Kathleen Davis ,  Chunyan Wang

IPC分类号： C12N5/00 ,  C12N5/09

CPC分类号： C12N5/0062 ,  C12N5/0068 ,  C12N5/0693 ,  C12N2533/40 ,  C12N2533/72

摘要： Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein.

公开(公告)号：US20180045681A1

公开(公告)日：2018-02-15

申请号：US15682070

申请日：2017-08-21

申请人： Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair

发明人： Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair

IPC分类号： G01N29/02 ,  G01N29/036

摘要： A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.

公开(公告)号：US20170137812A1

公开(公告)日：2017-05-18

申请号：US15350622

申请日：2016-11-14

申请人： Shyam S. Mohapatra ,  Srinivas Nagaraj Bharadwaj ,  Subhra Mohapatra

发明人： Shyam S. Mohapatra ,  Srinivas Nagaraj Bharadwaj ,  Subhra Mohapatra

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  A61K47/6807 ,  A61K47/6849 ,  A61K47/6929 ,  C12N5/0639 ,  C12N5/0645 ,  C12N5/0647 ,  C12N15/111 ,  C12N2310/141 ,  C12N2310/17 ,  C12N2320/30 ,  C12N2501/65 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Q2600/178

摘要： Provided are methods of treating an inflammatory disease in a subject in need thereof by administering an amount of microRNA 142, an amount of microRNA 223 or an amount of microRNA 142 and an amount of micro RNA 223 to the subject in need thereof.

公开(公告)号：US09624473B2

公开(公告)日：2017-04-18

申请号：US13775536

申请日：2013-02-25

申请人： Subhra Mohapatra ,  Shyam S. Mohapatra ,  Yvonne Kathleen Davis ,  Chunyan Wang

发明人： Subhra Mohapatra ,  Shyam S. Mohapatra ,  Yvonne Kathleen Davis ,  Chunyan Wang

IPC分类号： C12N5/00 ,  C12N5/09

CPC分类号： C12N5/0062 ,  C12N5/0068 ,  C12N5/0693 ,  C12N2533/40 ,  C12N2533/72

摘要： Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein.

公开(公告)号：US09161901B2

公开(公告)日：2015-10-20

申请号：US12918265

申请日：2009-02-23

申请人： Arun Kumar ,  Shyam S. Mohapatra ,  Don F. Cameron

发明人： Arun Kumar ,  Shyam S. Mohapatra ,  Don F. Cameron

IPC分类号： A61K35/48 ,  A61K9/00 ,  A61B5/00 ,  A61K9/50 ,  A61K9/51 ,  A61K31/12 ,  A61K47/36 ,  A61K47/46 ,  A61K47/48 ,  A61K49/00 ,  B82Y5/00

CPC分类号： A61K31/12 ,  A61B5/0071 ,  A61B5/4848 ,  A61K9/0019 ,  A61K9/5068 ,  A61K9/5161 ,  A61K47/36 ,  A61K47/46 ,  A61K47/61 ,  A61K47/6901 ,  A61K47/6939 ,  A61K49/0043 ,  A61K49/0093 ,  A61K49/0097 ,  B82Y5/00 ,  C12N5/0683

摘要： A method of delivering a compound of interest to the lungs of a subject by the intravenous injection of Sertoli cells loaded with a plurality of chitosan nanoparticles coupled with the compound of interest is provided. Testis-derived rat Sertoli cells were pre-loaded with chitosan nanoparticles coupled with or without the drug curcumin, pre-labeled with a fluorescent cell marker and then injected intravenously into the control or asthmatic mouse model host. Intact pre-loaded, pre-labeled Sertoli cells were present in the lungs at 15 minutes post-injection, appeared entrapped in the pulmonary pre-capillary vascular bed around alveolar sacs but were not present one hour post-injection although Sertoli cell label and cellular debris was. Most of the injected nanoparticle load (70%) and curcumin load (80%) was present in the lungs 15 minutes post-injection, and remained at 70% and 80%, respectively, one hour post-injection.

摘要翻译： 提供了通过静脉内注射负载有与感兴趣的化合物结合的多个壳聚糖纳米颗粒的Sertoli细胞将感兴趣的化合物递送到受试者的肺的方法。 睾丸衍生的大鼠支持细胞预先装载有或不含药物姜黄素的壳聚糖纳米颗粒，用荧光细胞标记物预先标记，然后静脉内注射到对照或哮喘小鼠模型宿主中。 完整的预加载，预先标记的Sertoli细胞在注射后15分钟存在于肺中，似乎被夹带在肺泡囊周围的肺前毛细血管血管床中，但注射后1小时不存在，尽管Sertoli细胞标记和细胞 碎片是。 在注射后15分钟，大部分注射的纳米粒子负载（70％）和姜黄色素负荷（80％）分别存在于肺中，并且在注射后1小时分别保持在70％和80％。

公开(公告)号：US09091640B1

公开(公告)日：2015-07-28

申请号：US14028920

申请日：2013-09-17

申请人： Shyam S. Mohapatra ,  Kyle A. Cissell ,  Subbiah Alwarappan

发明人： Shyam S. Mohapatra ,  Kyle A. Cissell ,  Subbiah Alwarappan

IPC分类号： G01N27/30

CPC分类号： G01N27/307 ,  B82Y30/00 ,  G01N27/48

摘要： Provided herein is a method for detecting mutations in polynucleotide sequences through the use of a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material (also referred to herein as a “CMG electrode.”) It is a surprising finding of the present invention that the CMG electrodes can be used to detect non-hybridization of capture and target polynucleotides using voltammetry, wherein non-hybridization indicates a mutation or difference in the target polynucleotide as compared to a control.

摘要翻译： 本文提供了通过使用涂覆有壳聚糖 - 石墨烯纳米片材料（本文也称为“CMG电极”）的玻璃碳电极（GCE）来检测多核苷酸序列中的突变的方法。这是令人惊奇的发现 本发明可以使用CMG电极来检测使用伏安法的捕获和靶多核苷酸的非杂交，其中非杂交指示与对照相比靶靶多核苷酸的突变或差异。

公开(公告)号：US20140004520A1

公开(公告)日：2014-01-02

申请号：US14000517

申请日：2012-03-16

申请人： Shyam S. Mohapatra ,  Jia-Wang Wang

发明人： Shyam S. Mohapatra ,  Jia-Wang Wang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/207

摘要： The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.

摘要翻译： 本发明提供用于检测，定量和/或高通量分析微小RNA的材料和方法。 有利地，本发明比其他目前可用的miRNA qPCR测定更灵敏和特异。 此外，本发明方便，易于使用，性价比高。 在一个实施方案中，本发明提供了用于所有miRNA逆转录的通用引物，用于PCR扩增反应的通用反向引物和通用探针。 在另一个实施方案中，本发明提供允许使用单次逆转录反应同时检测和/或定量多个靶miRNA的测定。

公开(公告)号：US20130144163A1

公开(公告)日：2013-06-06

申请号：US13486540

申请日：2012-06-01

申请人： Arun Kumar ,  Susana K. Lai-Yuen ,  Shyam S. Mohapatra

发明人： Arun Kumar ,  Susana K. Lai-Yuen ,  Shyam S. Mohapatra

IPC分类号： A61M31/00

CPC分类号： A61M31/00 ,  A61B17/42 ,  A61B17/43 ,  A61M2210/1408 ,  A61M2210/1425

摘要： A drug delivery device has been designed to directly deliver an agent to the ovaries through direct contact with the fallopian tubes. The device consists of three main components: a tubular inserter, a cylindrical chamber and a plunger. The device is a single-use applicator designed in a shape similar to a tampon to facilitate its insertion through the vagina and into the uterus. Positioning of the device centrally in the uterus is accomplished through the use of ultrasound. The chamber is inserted into the tubular inserter. Adjusting the length of the chamber inserted into the tubular inserter controls the amount of tubing released from the apertures in the tubular inserter. Ultrasound is used to ensure the proper placement of each tube at the entrance of each fallopian tube. The plunger is inserted into the chamber and adjustment of the plunger controls the amount of the agent released into the tubes.

摘要翻译： 药物输送装置被设计成通过与输卵管直接接触直接将药剂递送至卵巢。 该装置由三个主要部件组成：管状插入器，圆柱形腔和柱塞。 该装置是一种单一使用的涂抹器，其设计成类似于棉塞的形状，以便于其插入通过阴道并进入子宫。 通过使用超声波将器械定位在子宫中央。 将腔室插入管状插入器中。 调整插入到管状插入器中的腔室的长度控制从管状插入器中的孔释放的管道的量。 超声波用于确保每个管道在每个输卵管入口处的正确放置。 柱塞被插入到腔室中，并且柱塞的调节控制释放到管中的药剂的量。

公开(公告)号：US20120093781A1

公开(公告)日：2012-04-19

申请号：US13255599

申请日：2010-03-12

申请人： Shyam S. Mohapatra ,  Mark Glaum ,  Guoqing Liu

发明人： Shyam S. Mohapatra ,  Mark Glaum ,  Guoqing Liu

IPC分类号： A61K35/14 ,  C12Q1/68 ,  C12Q1/18 ,  G01N33/566 ,  C12N5/0787 ,  C12Q1/02

CPC分类号： C12N5/0642 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2510/00 ,  G01N33/5047

摘要： The subject invention pertains to the development of a novel human mast cell line, USF-MC1. USF-MC1 is a mast cell precursor present in human umbilical cord blood (HUCB) that may be sustained in culture in the absence of exogenous cytokines to serve as a convenient experimental model of human mast cell activation. The SCF-independent human mast cell line USF-MC1 responds to IgE-mediated and IgE-independent stimuli in a way comparable to that of LAD2. USF-MC1 cells are useful for investigation of IgE-mediated activation mechanisms of human mast cells, contributing to the development of effective treatments for allergic disorders and other disorders. The subject invention provides a ready source of human mast cells for research, including pharmacological studies for the screening of various agents, and toxicologic, e.g., for the cosmetic and pharmaceutical industries. The mast cells can also be used as biofactories, for the large-scale production of biomolecules.

摘要翻译： 本发明涉及新型人肥大细胞系USF-MC1的开发。 USF-MC1是存在于人脐带血（HUCB）中的肥大细胞前体，其可以在不存在外源性细胞因子的情况下在培养物中维持，以用作人肥大细胞活化的方便的实验模型。 与SCF无关的人肥大细胞系USF-MC1以与LAD2相当的方式对IgE介导的和IgE非依赖性刺激作出反应。 USF-MC1细胞可用于研究人肥大细胞的IgE介导的激活机制，有助于开发有效治疗过敏性疾病和其他疾病。 本发明提供了用于研究的人类肥大细胞的可用来源，包括用于筛选各种试剂的药理学研究，以及例如化妆品和制药工业的毒理学。 肥大细胞也可以用作生物物质，用于大规模生产生物分子。