公开(公告)号：US20230285442A1

公开(公告)日：2023-09-14

申请号：US17914225

申请日：2021-03-26

Applicant: Subhra MOHAPATRA ,  Shyam S. MOHAPATRA ,  Julio GARAY ,  Mazen HANNA ,  Karthick MAYILSAMY

Inventor： Subhra MOHAPATRA ,  Shyam S. MOHAPATRA ,  Julio GARAY ,  Mazen HANNA ,  Karthick MAYILSAMY

IPC: A61K31/722 ,  A61P31/12

CPC classification number: A61K31/722 ,  A61P31/12

Abstract: Described herein are methods for neutralizing a virus. The methods involve interacting the virus with an oligochitosan or a derivative thereof having a molecular weight of at least 1 KDa and a degree of deacetylation of at least 1%. The methods described herein have numems applications with respect to the treatment or prevention of viral infections caused by a human immunodeficiency virus, a paramyxovirus, an orthomyxovirus, a coronavirus, or a filovirus as well as the detection and quantification of viral load. Also described herein are the synthesis of the oligochitosan and derivatives thereof.

公开(公告)号：US09439978B2

公开(公告)日：2016-09-13

申请号：US14346330

申请日：2012-09-28

Applicant: Subhra Mohapatra ,  Chunyan Wang

Inventor： Subhra Mohapatra ,  Chunyan Wang

IPC: A61K48/00 ,  A61K47/48 ,  A61K49/18 ,  C12N15/88 ,  C12N15/85

CPC classification number: A61K48/0041 ,  A61K47/48192 ,  A61K47/4823 ,  A61K47/48907 ,  A61K47/48923 ,  A61K47/59 ,  A61K47/61 ,  A61K47/6935 ,  A61K47/6939 ,  A61K48/0033 ,  A61K49/1809 ,  A61K49/1857 ,  A61K49/1863 ,  A61K49/1875 ,  C12N15/85 ,  C12N15/88 ,  C12N2800/107 ,  C12N2810/859

Abstract: Provided herein are compositions comprising a micelle having a hydrophobic superparmagnetic iron oxide nanoparticle (SPION) core, a first coating comprising a cationic polymer, and a second coating comprising a polynucleotide. Also provided are methods of using the compositions for transfection and/or transformation of a cell with the polynucleotide. Further provided are methods of detecting transfection of a cell with the polynucleotide.

Abstract translation: 本文提供了包含具有疏水超磁性氧化铁纳米颗粒（SPION）芯的胶束，包含阳离子聚合物的第一涂层和包含多核苷酸的第二涂层的组合物。 还提供了使用组合物用于与多核苷酸转染和/或转化细胞的方法。 还提供了用多核苷酸检测细胞转染的方法。

公开(公告)号：US20150024967A1

公开(公告)日：2015-01-22

申请号：US14447779

申请日：2014-07-31

Applicant: Subhra Mohapatra ,  Shyam S. Mohapatra

Inventor： Subhra Mohapatra ,  Shyam S. Mohapatra

IPC: G01N33/50 ,  C12N5/09

CPC classification number: G01N33/5011 ,  C12N5/0068 ,  C12N5/0693 ,  C12N2533/40 ,  C12N2533/72 ,  G01N33/5082 ,  G01N2500/10

Abstract: Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein.

Abstract translation: 本文提供了包含无规取向纤维的三维支架组合物，其中纤维包含聚乙二醇 - 聚乳酸嵌段共聚物（PEG-PLA）和聚（乳酸 - 共 - 乙醇酸）（PLGA）。 还提供了使用本文所述的三维支架的方法。

公开(公告)号：US20140343120A1

公开(公告)日：2014-11-20

申请号：US14102793

申请日：2013-12-11

Applicant: SHYAM S. MOHAPATRA ,  WEIDONG XU ,  XIAOYUAN KONG ,  XIAOQIN WANG ,  SUBHRA MOHAPATRA

Inventor： SHYAM S. MOHAPATRA ,  WEIDONG XU ,  XIAOYUAN KONG ,  XIAOQIN WANG ,  SUBHRA MOHAPATRA

IPC: C12N15/113 ,  A61K31/713 ,  A61K47/48

CPC classification number: C12N15/1138 ,  A61K9/0019 ,  A61K9/0034 ,  A61K9/0043 ,  A61K9/0073 ,  A61K9/5161 ,  A61K31/711 ,  A61K31/713 ,  A61K47/61 ,  A61K47/6935 ,  A61K47/6939 ,  A61K48/0008 ,  A61K48/005 ,  A61K49/0008 ,  B82Y5/00 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2320/30

Abstract: Methods, compositions and devices are provided by the present invention for reducing activity of a natriuretic peptide receptor and other signals. Therapeutic treatments are provided by use of polynucleotides encoding a natriuretic peptide or by regulating the expression of natriuretic peptide receptor, such as NPRA and NPRC, or combinations of these therapies. Routes used for delivering polynucleotides encoding a natriuretic peptide, or, for example, siRNA that down regulates natriuretic peptide receptor include subcutaneous injection, oral gavage, transdermal and intranasal delivery routes. Compositions can include chitosan, chitosan derivatives, and chitosan derivative and a lipid. Transdermal delivery can use a transdermal cream. Intranasal delivery can use a dropper or an aspirator for delivery of a mist. Oral gavage delivers equivalent to oral delivery. Delivery permits cell and tissue specific targeting of gene therapies resulting in expression of a natriuretic peptide or down regulation of natriuretic peptide receptor. A variety of cancers, asthma and viral diseases can be treated therapeutically using the methods and compositions of the present invention.

Abstract translation: 本发明提供了降低利尿钠肽受体活性和其他信号的方法，组合物和装置。 通过使用编码利尿钠肽的多核苷酸或通过调节利尿钠肽受体（如NPRA和NPRC）的表达或这些疗法的组合来提供治疗性治疗。 用于递送编码利尿钠肽的多核苷酸的路线，或例如下调调节利尿钠肽受体的siRNA包括皮下注射，口服管饲，透皮和鼻内递送途径。 组合物可以包括壳聚糖，壳聚糖衍生物和壳聚糖衍生物和脂质。 透皮给药可以使用透皮霜。 鼻内输送可以使用滴管或吸气器来传送雾。 口服管子相当于口服输送。 交付允许基因治疗的细胞和组织特异性靶向导致利尿钠肽的表达或利尿钠肽受体的下调。 可以使用本发明的方法和组合物治疗多种癌症，哮喘和病毒性疾病。

公开(公告)号：US20120270923A1

公开(公告)日：2012-10-25

申请号：US13422880

申请日：2012-03-16

Applicant: Subhra Mohapatra ,  Shyam Mohapatra

Inventor： Subhra Mohapatra ,  Shyam Mohapatra

IPC: C12Q1/68 ,  C07K16/18 ,  C12N5/09 ,  A61K31/7088 ,  G01N33/53 ,  C12Q1/02

CPC classification number: G01N33/57415 ,  C07K16/286 ,  C07K2317/34 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/12 ,  C12N2310/14 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57419 ,  G01N33/5743 ,  G01N33/57434 ,  G01N33/57438 ,  G01N33/57446 ,  G01N33/57492 ,  G01N2333/72 ,  G01N2800/50 ,  G01N2800/52 ,  G01N2800/56

Abstract: The invention pertains to biomarkers for clinical detection of malignancies, especially for early detection of cancers. More specifically, this invention pertains to the role of Natriuretic Peptide Receptor A (NPRA) in cancer (e.g., tumor) progression. Thus, the invention includes materials and methods for the detection and prognosis of malignancies. The invention also pertains to methods for treating malignancies.

Abstract translation: 本发明涉及用于临床检测恶性肿瘤的生物标志物，特别是癌症的早期检测。 更具体地，本发明涉及利钠肽受体A（NPRA）在癌症（例如肿瘤）进展中的作用。 因此，本发明包括用于检测和预测恶性肿瘤的材料和方法。 本发明还涉及治疗恶性肿瘤的方法。

公开(公告)号：US20220395477A1

公开(公告)日：2022-12-15

申请号：US17773257

申请日：2020-10-29

Applicant: Shyam S. MOHAPATRA ,  Subhra MOHAPATRA ,  Andrew Robert MCGILL ,  Eleni MARKOUTSA

Inventor： Shyam S. MOHAPATRA ,  Subhra MOHAPATRA ,  Andrew Robert MCGILL ,  Eleni MARKOUTSA

IPC: A61K31/19

Abstract: The present disclosure relates to compositions and uses thereof for preventing and treating respiratory syncytial vims (RSV) infection. In some aspects, disclosed herein is a method for preventing or treating respiratory syncytial vims (RSV) infection in a subject, comprising administering to the subject a therapeutically effective amount of a composition comprising a short-chain fatty acid.

公开(公告)号：US11110183B2

公开(公告)日：2021-09-07

申请号：US16531734

申请日：2019-08-05

Applicant: Shyam S. Mohapatra ,  Subhra Mohapatra ,  Eleni Markousta

Inventor： Shyam S. Mohapatra ,  Subhra Mohapatra ,  Eleni Markousta

IPC: C07H21/04 ,  A61K47/69 ,  A61K47/60 ,  A61P27/02 ,  A61K9/00 ,  C12N15/113 ,  A61K31/7088 ,  A61K31/4439 ,  A61K47/61 ,  A61K47/62 ,  A61K47/54

Abstract: A multifunctional dendrimer nanoparticle and method of treating diseases of the posterior segment of the eye is presented. The functionalized polyamidoamine (PAMAM) dendrimer effectively delivers drugs and/or genes to the posterior eye, thereby providing for the effective, non-invasive, and topical treatment of diseased in the posterior eye. The multifunctional dendrimer nanoparticle has shRNA-encoding DNA and small molecule drug encapsulated cyclodextrin complexed to the outer surface of the dendrimer for delivery to the posterior segment of the eye.

公开(公告)号：US11016062B2

公开(公告)日：2021-05-25

申请号：US16722210

申请日：2019-12-20

Applicant: Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair ,  Tao Wang

Inventor： Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair ,  Tao Wang

IPC: G01N29/02 ,  G01N29/036 ,  G01N29/22 ,  G01N29/24 ,  G01N33/48

Abstract: A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.

公开(公告)号：US10520472B2

公开(公告)日：2019-12-31

申请号：US15682070

申请日：2017-08-21

Applicant: Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair ,  Tao Wang

Inventor： Shyam S. Mohapatra ,  Subhra Mohapatra ,  Rasim Oytun Guldiken ,  Rajesh R. Nair ,  Tao Wang

IPC: G01N29/02 ,  G01N29/036 ,  G01N33/48 ,  G01N29/24 ,  G01N29/22

Abstract: A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.

公开(公告)号：US09434926B1

公开(公告)日：2016-09-06

申请号：US14627671

申请日：2015-02-20

Applicant: Subhra Mohapatra ,  Chunyan Wang

Inventor： Subhra Mohapatra ,  Chunyan Wang

IPC: A01N63/00 ,  A01N65/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0775 ,  A61K35/28 ,  A61K9/06 ,  A61K47/02 ,  A61K47/36 ,  A61K47/14

CPC classification number: C12N5/0663 ,  A61K9/06 ,  A61K35/28 ,  A61K47/02 ,  A61K47/14 ,  A61K47/36 ,  A61L27/20 ,  A61L27/3821 ,  A61L27/52 ,  C08B37/003 ,  C08F220/54 ,  C08F222/1006 ,  C08F251/00 ,  C08F290/062 ,  C08F292/00 ,  C08G65/3322 ,  C08G83/001 ,  C08J3/075 ,  C08J2387/00 ,  C08L5/08 ,  C08L71/02 ,  C12N5/0068 ,  C12N2501/00 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/72 ,  A61L27/443 ,  C08L33/00 ,  A61L15/60 ,  C08F220/52 ,  C08F220/06 ,  C08F2222/1013

Abstract: Provided herein are hydrogel compositions containing graphene, chitosan, a polyethylene (glycol) diacrylate (PEGDA). The hydrogel can optionally contain an N-isopropylacrylamide (NIPAM) (TPCG hydrogel). Also provided are methods for differentiating a cell on the hydrogels described herein. Further provided herein are methods for delivering a compound via the hydrogels described herein.

Abstract translation: 本文提供含有石墨烯，壳聚糖，聚乙二醇（二醇）二丙烯酸酯（PEGDA）的水凝胶组合物。 水凝胶可任选地含有N-异丙基丙烯酰胺（NIPAM）（TPCG水凝胶）。 还提供了用于区分本文所述的水凝胶上的细胞的方法。 本文还提供了通过本文所述的水凝胶递送化合物的方法。