公开(公告)号：US20100037993A1

公开(公告)日：2010-02-18

申请号：US12276725

申请日：2008-11-24

申请人： So Youn Kim ,  Seung Hyun Hong

发明人： So Youn Kim ,  Seung Hyun Hong

IPC分类号： C22C38/04 ,  C21D6/00

CPC分类号： C22C38/02 ,  C21D8/0405 ,  C21D8/0421 ,  C21D2211/001 ,  C22C38/04 ,  C22C38/06

摘要： The present invention provides a TWIP steel sheet that has an austenite matrix structure and includes 15-25 wt % of manganese. The TWIP steel sheet has a recrystallization texture in which brass orientation is suppressed and {3 5 2} is developed as its main orientation during heat treatment subsequent to cold rolling, and has an average plastic strain ratio of 1.2 or more, preferably 1.5 or more. Since this TWIP steel sheet has high workability and strength, complicated vehicle body components can be easily manufactured through a press molding process without cracking or rupturing.

摘要翻译： 本发明提供一种具有奥氏体矩阵结构并包含15-25重量％锰的TWIP钢板。 TWIP钢板具有抑制黄铜取向的再结晶织构，并且在冷轧之后的热处理中开发{3 5 2} <2 2 1>作为其主要取向，并且其平均塑性应变比为1.2以上 ，优选为1.5以上。 由于该TWIP钢板具有高的可加工性和强度，因此可以通过压制成型工艺容易地制造复杂的车体部件而不会发生裂纹或破裂。

公开(公告)号：US10132801B2

公开(公告)日：2018-11-20

申请号：US13322651

申请日：2010-05-28

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： C40B20/02 ,  C40B30/04 ,  G01N33/68 ,  G01N33/543 ,  C12N15/09

摘要： The present invention relates to a method for screening a substance inhibiting protein-protein interactions, and more particularly to a method for screening a substance inhibiting protein-protein interactions, the method comprising using a protein chip having immobilized thereon spots comprising a mixture of a sol-gel material and a protein. According to the invention, a protein chip can be easily manufactured in a 96-well plate using a sol-gel material, whereby an inhibitor that inhibits protein-protein interactions can be easily screened from a library of natural substances.

公开(公告)号：US10191045B2

公开(公告)日：2019-01-29

申请号：US15065494

申请日：2016-03-09

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： G01N33/543 ,  B01J19/00

摘要： The present invention relates to a method for screening a sol composition for sol-gel biochips, which is used to immobilize a probe on a surface-untreated substrate, also relates to a sol composition screened by said method and a sol-gel biochip comprising said sol composition immobilized thereon. The sol composition screened by the disclosed method can be mixed with a probe, and the sol mixture can be integrated on 96-well plates, which are widely used in existing bioassays, without surface treatment. Also, the biochip can provide a sensitive and specific good analysis results because this immobilization methods of probe maintain the nature of probes without modification.

公开(公告)号：US20120129722A1

公开(公告)日：2012-05-24

申请号：US13322651

申请日：2010-05-28

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： C40B30/04

CPC分类号： G01N33/54306 ,  C40B20/02 ,  C40B30/04 ,  G01N33/6845

摘要： The present invention relates to a method for screening a substance inhibiting protein-protein interactions, and more particularly to a method for screening a substance inhibiting protein-protein interactions, the method comprising using a protein chip having immobilized thereon spots comprising a mixture of a sol-gel material and a protein. According to the invention, a protein chip can be easily manufactured in a 96-well plate using a sol-gel material, whereby an inhibitor that inhibits protein-protein interactions can be easily screened from a library of natural substances.

摘要翻译： 本发明涉及一种筛选抑制蛋白质 - 蛋白质相互作用的物质的方法，更具体地说，涉及一种用于筛选抑制蛋白质 - 蛋白质相互作用的物质的方法，所述方法包括使用固定有蛋白质芯片的蛋白质芯片，所述蛋白质芯片包含溶胶 凝胶材料和蛋白质。 根据本发明，可以使用溶胶 - 凝胶材料容易地在96孔板中制造蛋白质芯片，从而可以容易地从天然物质文库中筛选抑制蛋白质 - 蛋白质相互作用的抑制剂。

公开(公告)号：US20120028811A1

公开(公告)日：2012-02-02

申请号：US13059223

申请日：2009-08-17

申请人： Harold G. Craighead ,  John T. Lis ,  Seungmin Park ,  So Youn Kim ,  Jiyoung Ahn ,  Minjoung Jo

发明人： Harold G. Craighead ,  John T. Lis ,  Seungmin Park ,  So Youn Kim ,  Jiyoung Ahn ,  Minjoung Jo

IPC分类号： C40B10/00 ,  C07H21/00 ,  B32B37/14 ,  G03F7/20 ,  B32B37/02 ,  C40B60/08 ,  C40B40/06

CPC分类号： C12Q1/6811 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0487 ,  C12Q1/686 ,  C12Q2525/205 ,  Y10T156/10 ,  C12Q2565/629 ,  C12Q2541/101

摘要： The present invention relates to microfluidic chips and their use in SELEX. The microfluidic chip preferably includes a reaction chamber that contains a high surface area material that contains target. One preferred high surface area material is a sol-gel derived material. Methods of making the microfluidic chips are described herein, as are uses of these devices to select aptamers against the target.

摘要翻译： 本发明涉及微流控芯片及其在SELEX中的应用。 微流体芯片优选包括含有包含靶的高表面积材料的反应室。 一种优选的高表面积材料是溶胶 - 凝胶衍生材料。 这里描述了制造微流体芯片的方法，以及这些装置用于选择针对靶的适体的方法。

公开(公告)号：US20120040865A1

公开(公告)日：2012-02-16

申请号：US13201764

申请日：2010-02-16

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： C40B30/06 ,  C07H21/04 ,  G01N33/566

CPC分类号： G01N33/5438 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6825 ,  C12Q2525/205

摘要： The present invention relates to a method and kit of detecting a target material using an aptamer, and more particularly to a method and kit for detecting a target material, in which a sample and a second aptamer are added to a first aptamer immobilized on a solid phase so as to form a bond sandwiched between the first aptamer, the target material and the second aptamer, to an FET sensor-based method and kit for detecting a target material, and to an AAO sensor-based method and kit for detecting a target material.The inventive method for a target material using an aptamer can detect even low-molecular-weight materials which were difficult to detect in the prior art, thereby enabling detection of disease-related metabolites, environmental pollutants and food toxins in solutions. In addition, the detection method of the present invention is a direct and simple method and is highly cost-effective, because it uses the aptamer which can be consistently reproduced and can be produced at low costs. Thus, the present invention is very useful.

摘要翻译： 本发明涉及使用适配体检测靶物质的方法和试剂盒，更具体地说，涉及用于检测目标物质的方法和试剂盒，其中将样品和第二适体加入固定在固体上的第一适配体 以形成夹在第一适体，靶材料和第二适体之间的键，用于检测目标材料的基于FET传感器的方法和试剂盒，以及用于检测靶的AAO传感器的方法和试剂盒 材料。 使用适体的目标材料的本发明方法可以检测甚至在现有技术中难以检测的低分子量材料，从而能够在溶液中检测疾病相关代谢物，环境污染物和食物毒素。 此外，本发明的检测方法是直接和简单的方法，并且是高度成本有效的，因为它使用可以一致再现并可以以低成本生产的适配体。 因此，本发明是非常有用的。

公开(公告)号：US20120015354A1

公开(公告)日：2012-01-19

申请号：US13201797

申请日：2010-02-16

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07C37/68 ,  G01N33/02 ,  G01N33/24 ,  G01N33/00 ,  C07H21/04 ,  G01N33/18

CPC分类号： C12N15/115 ,  C12N2310/16

摘要： A nucleic acid aptamer capable of binding specifically to bisphenol A and a method of detecting and removing bisphenol A using the nucleic acid aptamer. The nucleic acid aptamer capable of binding specifically to bisphenol A has affinity for bisphenol A at nM concentration, and thus can detect even a very small amount of bisphenol A. Also, the nucleic acid aptamer can specifically detect only bisphenol A without showing affinity for other bisphenols, including bisphenol B having no difference from bisphenol A except for a single methyl group. Accordingly, the nucleic acid aptamer is effective in detecting and removing the environmental hormone bisphenol A which is difficult to detect by conventional methods.

摘要翻译： 能够特异性结合双酚A的核酸适体和使用核酸适体检测和除去双酚A的方法。 能够与双酚A特异性结合的核酸适配体在nM浓度下对双酚A具有亲和性，因此可以检测到甚至非常少量的双酚A。另外，核酸适体可以特异性地检测双酚A而不显示对其他的亲和力 双酚，包括除双酚A之外没有单独甲基的双酚B。 因此，核酸适体在检测和去除通过常规方法难以检测的环境激素双酚A方面是有效的。

公开(公告)号：US10221412B2

公开(公告)日：2019-03-05

申请号：US13877862

申请日：2011-10-05

申请人： So Youn Kim ,  Ji-Young Ahn ,  Minjoung Jo ,  Tae Kyung Kim

发明人： So Youn Kim ,  Ji-Young Ahn ,  Minjoung Jo ,  Tae Kyung Kim

IPC分类号： C12N15/10 ,  C40B30/04 ,  C12N15/11 ,  C12N15/115 ,  G01N33/53

摘要： The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques. Particularly, when a process for selecting aptamers is performed using the device of the invention together with a high-throughput sequencing method, the number of target binding/elution/amplification rounds can be greatly reduced, and the process can be performed in an automated manner. Thus, the device of the invention is highly useful.

公开(公告)号：US20130274113A1

公开(公告)日：2013-10-17

申请号：US13877862

申请日：2011-10-05

申请人： So Youn Kim ,  Ji-Young Ahn ,  Minjoung Jo ,  Tae Kyung Kim

发明人： So Youn Kim ,  Ji-Young Ahn ,  Minjoung Jo ,  Tae Kyung Kim

IPC分类号： C12N15/10

CPC分类号： C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/11 ,  C12N2320/13 ,  G01N33/5308 ,  G01N2500/00

摘要： The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques. Particularly, when a process for selecting aptamers is performed using the device of the invention together with a high-throughput sequencing method, the number of target binding/elution/amplification rounds can be greatly reduced, and the process can be performed in an automated manner. Thus, the device of the invention is highly useful.

摘要翻译： 本发明涉及用于选择核酸适体的多重微流体装置和使用其的核酸适配体的高通量选择方法，更具体地说，涉及一种复合微流体装置（SELEX lap-on-a-chip），其中 使用改进的多路复用平台代替单个靶的适配体的开发，以及使用其的高通量测序的适配体的高通量选择的方法。 根据本发明的多重微流体装置可以同时检测多个靶的适体，并且与传统的多重技术相比，其可大大增加筛选通过量并大大缩短处理时间。 特别地，当使用本发明的装置和高通量测序方法进行选择适体的方法时，可以大大减少目标结合/洗脱/放大倍数的数量，并且可以以自动方式进行处理 。 因此，本发明的装置是非常有用的。

公开(公告)号：US20160334400A1

公开(公告)日：2016-11-17

申请号：US15065494

申请日：2016-03-09

申请人： So Youn Kim

发明人： So Youn Kim

IPC分类号： G01N33/543 ,  B01J19/00

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/0061 ,  B01J2219/00623 ,  B01J2219/00725 ,  B01J2219/00756

摘要： The present invention relates to a method for screening a sol composition for sol-gel biochips, which is used to immobilize a probe on a surface-untreated substrate, also relates to a sol composition screened by said method and a sol-gel biochip comprising said sol composition immobilized thereon. The sol composition screened by the disclosed method can be mixed with a probe, and the sol mixture can be integrated on 96-well plates, which are widely used in existing bioassays, without surface treatment. Also, the biochip can provide a sensitive and specific good analysis results because this immobilization methods of probe maintain the nature of probes without modification.

摘要翻译： 本发明涉及一种用于筛选溶胶 - 凝胶生物芯片的溶胶组合物的方法，其用于将探针固定在未经表面处理的基材上，还涉及通过所述方法筛选的溶胶组合物和包含所述溶胶 - 凝胶生物芯片的溶胶 - 凝胶生物芯片 固定在其上的溶胶组合物。 通过所公开的方法筛选的溶胶组合物可以与探针混合，并且溶胶混合物可以整合在96孔板上，其广泛用于现有的生物测定中，无需表面处理。 此外，生物芯片可以提供灵敏和具体的良好分析结果，因为探针的固定方法保持探针的性质而无需修改。