公开(公告)号：US06221634B1

公开(公告)日：2001-04-24

申请号：US09487308

申请日：2000-01-19

申请人： Sonoko Takeuchi ,  Naoto Tonouchi ,  Kenzo Yokozeki

发明人： Sonoko Takeuchi ,  Naoto Tonouchi ,  Kenzo Yokozeki

IPC分类号： C12P1900

CPC分类号： C12P7/18 ,  C12P19/02

摘要： Xylitol or D-xylulose is produced through direct fermentation from glucose by culturing a microorganism belonging to the genus Gluconobacter, Acetobacter or Frateuria, and having an ability to produce xylitol or D-xylulose in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.

摘要翻译： 木糖醇或D-木酮糖通过葡萄糖直接发酵生产，通过培养属于葡糖酸杆菌属，醋酸杆菌属或葡萄糖酵母属的微生物，并且具有在合适的培养基中生产木糖醇或D-木酮糖以在木糖醇或D-木酮糖中积累木糖醇或D-木酮糖的能力 培养基，并从培养基中收集木糖醇或D-木酮糖。

公开(公告)号：US06335177B1

公开(公告)日：2002-01-01

申请号：US09347001

申请日：1999-07-02

申请人： Yasuhiro Mihara ,  Sonoko Takeuchi ,  Yasuko Jojima ,  Naoto Tonouchi ,  Ryosuke Fudou ,  Kenzo Yokozeki

发明人： Yasuhiro Mihara ,  Sonoko Takeuchi ,  Yasuko Jojima ,  Naoto Tonouchi ,  Ryosuke Fudou ,  Kenzo Yokozeki

IPC分类号： C12P100

CPC分类号： C12R1/01 ,  C12P7/06 ,  C12P7/18 ,  C12P19/02 ,  Y02E50/17

摘要： According to the present invention, there are provided microorganisms having an ability to producing xylitol or D-xylulose by fermentation, and a method for producing xylitol or D-xylulose using the microorganisms. Osmophilic microorganisms were collected from soil, and the obtained microorganisms were searched for a bacterium having an ability to produce xylitol or D-xylulose from glucose. Xylitol or D-xylulose is produced by culturing an isolated bacterium in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.

摘要翻译： 根据本发明，提供了通过发酵生产木糖醇或D-木酮糖的能力的微生物，以及使用微生物生产木糖醇或D-木酮糖的方法。 从土壤中收集渗透性微生物，并从所得微生物中搜索具有从葡萄糖产生木糖醇或D-木酮糖的能力的细菌。 通过在合适的培养基中培养分离的细菌以在培养基中积累木糖醇或D-木酮糖并从培养基中收集木糖醇或D-木酮糖来生产木糖醇或D-木酮糖。

公开(公告)号：US20120003693A1

公开(公告)日：2012-01-05

申请号：US13211834

申请日：2011-08-17

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/00 ,  C12N1/15 ,  C12N9/00 ,  C12N1/19 ,  C07H21/04 ,  C12N1/21

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US07531340B2

公开(公告)日：2009-05-12

申请号：US11841211

申请日：2007-08-20

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12N9/10 ,  C12N15/00 ,  C12P13/04 ,  C12P21/04 ,  C12Q1/00 ,  C12Q1/48 ,  C07H21/04 ,  C12Q1/68 ,  C12N1/20

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US06777208B2

公开(公告)日：2004-08-17

申请号：US09983657

申请日：2001-10-25

申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

IPC分类号： C12P1938

CPC分类号： C12P19/38 ,  C12P19/40

摘要： A 2′-deoxyribonucleoside is produced by culturing a microorganism, which is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA, in a medium in which the microorganism can grow to produce the 2′-deoxyribonucleoside.

摘要翻译： 通过培养用编码核糖核苷酸还原酶的基因转化的微生物，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因而降低或消除2'-脱氧核糖核苷降解活性的2'-脱氧核糖核苷 其中微生物能够生长以产生2'-脱氧核糖核苷的培养基。

公开(公告)号：US06242228B1

公开(公告)日：2001-06-05

申请号：US09363189

申请日：1999-07-29

申请人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

发明人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

IPC分类号： C12P718

CPC分类号： C12N9/0006 ,  C12P7/18

摘要： Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

摘要翻译： 通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA（其是以下（A）或（B）的蛋白质）作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇：（A） 具有SEQ ID NO：4的氨基酸序列;（B）具有SEQ ID NO：4的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或倒位，并且具有木糖醇 脱氢酶活性。

公开(公告)号：US08389241B2

公开(公告)日：2013-03-05

申请号：US13611827

申请日：2012-09-12

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US08329428B2

公开(公告)日：2012-12-11

申请号：US13211834

申请日：2011-08-17

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20100267082A1

公开(公告)日：2010-10-21

申请号：US12729656

申请日：2010-03-23

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/21 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US20080166761A1

公开(公告)日：2008-07-10

申请号：US11841151

申请日：2007-08-20

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N5/00

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。