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21 条记录 (耗时 0.033 秒)

    METHODS FOR DETECTING FETAL NUCLEIC ACIDS AND DIAGNOSING FETAL ABNORMALITIES
    2.
    发明申请
    METHODS FOR DETECTING FETAL NUCLEIC ACIDS AND DIAGNOSING FETAL ABNORMALITIES 审中-公开
    用于检测天然核酸的方法和诊断宫颈异常

    公开(公告)号：US20130022977A1

    公开(公告)日：2013-01-24

    申请号：US13619039

    申请日：2012-09-14

    申请人： Stanley Lapidus ,  John F. Thompson ,  Doron Lipson ,  Patrice Milos ,  J. William Efcavitch ,  Stanley Letovsky

    发明人： Stanley Lapidus ,  John F. Thompson ,  Doron Lipson ,  Patrice Milos ,  J. William Efcavitch ,  Stanley Letovsky

    IPC分类号： C12Q1/68 ,  B82Y15/00

    摘要： The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.

    摘要翻译： 本发明一般涉及用于检测胎儿核酸的方法和用于诊断胎儿异常的方法。 在某些实施方案中，本发明提供了用于确定胎儿核酸是否存在于母体样品中的方法，包括获得怀疑包括胎儿核酸的母体样品，并对样品进行测序反应以确定至少部分 Y染色体，从而确定胎儿核酸存在于样品中。 在其它实施方案中，本发明提供用于定量或定性分析以检测母体样品中的胎儿核酸的方法，而不管检测Y染色体的能力，特别是对于包括来自雌性胎儿的正常核酸的样品的检测能力。

    POLYMERASES AND METHODS OF USE THEREOF
    3.
    发明申请
    POLYMERASES AND METHODS OF USE THEREOF 审中-公开
    聚合物及其使用方法

    公开(公告)号：US20100203524A1

    公开(公告)日：2010-08-12

    申请号：US12609543

    申请日：2009-10-30

    申请人： J. William Efcavitch ,  Jayson L. Bowers ,  Philip R. Buzby ,  John F. Thompson

    发明人： J. William Efcavitch ,  Jayson L. Bowers ,  Philip R. Buzby ,  John F. Thompson

    IPC分类号： C12Q1/68 ,  C12N9/10

    CPC分类号： C12P19/34 ,  C12N9/1241 ,  C12Q1/6869

    摘要： The invention generally relates to polymerases for efficient and controlled sequencing-by-synthesis reactions. In certain embodiments, the invention provides a polymerase enzyme including at least one mutation that enhances ability of the polymerase as compared to a wild-type polymerase to incorporate a nucleotide into a nascent strand of DNA or cDNA including at least one modified nucleotide.

    摘要翻译： 本发明一般涉及用于有效和控制的合成反应的聚合酶。 在某些实施方案中，本发明提供了聚合酶，其包括与野生型聚合酶相比增强聚合酶能力的至少一个突变，以将核苷酸掺入包含至少一个修饰的核苷酸的DNA或cDNA的新生链中。

    Polymers for separation of biomolecules by capillary electrophoresis
    6.
    发明授权
    Polymers for separation of biomolecules by capillary electrophoresis 失效

    公开(公告)号：US5552028A

    公开(公告)日：1996-09-03

    申请号：US458525

    申请日：1995-06-02

    申请人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    发明人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    IPC分类号： C08F20/52 ,  C08F26/10 ,  C08F122/38 ,  C08F126/10 ,  C08G63/06 ,  C08G63/08 ,  G01N27/447 ,  C07K1/26 ,  B01J13/00 ,  C25B7/00

    CPC分类号： G01N27/44752 ,  G01N27/44747

    摘要： The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20.degree. C. to about 50.degree. C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5.times.10.sup.3 to about 1.times.10.sup.6 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

    Polymers for separation of biomolecules by capillary electrophoresis
    7.
    发明授权
    Polymers for separation of biomolecules by capillary electrophoresis 失效

    公开(公告)号：US5567292A

    公开(公告)日：1996-10-22

    申请号：US350852

    申请日：1994-12-06

    申请人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    发明人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    IPC分类号： C08F20/52 ,  C08F26/10 ,  C08F122/38 ,  C08F126/10 ,  C08G63/06 ,  C08G63/08 ,  G01N27/447 ,  C07K1/26 ,  C25B7/00 ,  C25B15/00

    CPC分类号： G01N27/44752 ,  G01N27/44747

    摘要： The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20.degree. C. to about 50.degree. C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5.times.10.sup.3 to about 1.times.10.sup.6 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

    Polymers for separation of biomolecules by capillary electrophoresis
    10.
    发明授权
    Polymers for separation of biomolecules by capillary electrophoresis 有权
    通过毛细管电泳分离生物分子的聚合物

    公开(公告)号：US06358385B1

    公开(公告)日：2002-03-19

    申请号：US09228991

    申请日：1999-01-11

    申请人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    发明人： Ramakrishna S. Madabhushi ,  Steven M. Menchen ,  J. William Efcavitch ,  Paul D. Grossman

    IPC分类号： B01D5702

    CPC分类号： G01N27/44752 ,  G01N27/44747

    摘要： The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

    摘要翻译： 本发明提供了不带电的水溶性二氧化硅吸附聚合物，用于抑制毛细管电泳中的电渗流和降低毛细管电泳中的分析物 - 壁相互作用。 在本发明的一个方面，一种或多种这样的聚合物用作用于通过毛细管电泳分离生物分子例如多核苷酸，多糖，蛋白质等的分离介质的组分。 通常，这种聚合物的特征在于（i）在约20℃至约50℃之间的温度范围内的水溶性，（ii）分离介质中的浓度在约0.001％至约10％（重量） /体积），（iii）约5×10 3至约1×10 6道尔顿范围内的分子量，和（iv）在pH为约6至约9范围内的水性介质中不存在带电基团。在一个实施方案中， 本发明选自聚内酰胺类，如聚乙烯吡咯烷酮; N，N-二取代的聚丙烯酰胺; 和N-取代的聚丙烯酰胺。 根据本发明的方法，足够量的聚合物吸附到毛细管表面以建立一个高粘度的区域，从而将分析物与壁隔离并阻止双电层在电场下移动。