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20 条记录 (耗时 0.02 秒)

    Recombinant expression of insulin C-peptide
    1.
    发明授权
    Recombinant expression of insulin C-peptide 失效
    胰岛素C肽的重组表达

    公开(公告)号：US06558924B1

    公开(公告)日：2003-05-06

    申请号：US09485286

    申请日：2000-02-07

    申请人： Stefan Stahl ,  Mathias Uhlen ,  Per-Ake Nygren ,  Per Jonasson

    发明人： Stefan Stahl ,  Mathias Uhlen ,  Per-Ake Nygren ,  Per Jonasson

    IPC分类号： C12N1500

    CPC分类号： C12N15/62 ,  C07K14/62 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/705 ,  C07K2319/75

    摘要： The present invention provides a method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of the insulin C-peptide, and cleaving the expressed polypeptide to release single copies of the insulin C-peptide. Also provided are nucleic acid molecules, expression vectors and host cells, for use in such a method and the multimeric insulin C-peptide polypeptide expressed and cleaved in such a method.

    摘要翻译： 本发明提供了一种生产胰岛素C肽的方法，其包括在宿主细胞中表达包含胰岛素C-肽的多个拷贝的多聚体多肽，并切割表达的多肽以释放胰岛素C肽的单拷贝。 还提供了用于这种方法的核酸分子，表达载体和宿主细胞，并且以这种方法表达和切割多聚胰岛素C肽多肽。

    DEVICE AND METHOD FOR THE PRODUCTION OF COOLED COFFEE
    7.
    发明申请
    DEVICE AND METHOD FOR THE PRODUCTION OF COOLED COFFEE 有权
    用于生产冷却咖啡的装置和方法

    公开(公告)号：US20110014339A1

    公开(公告)日：2011-01-20

    申请号：US12836824

    申请日：2010-07-15

    申请人： Stefan Stahl ,  Mariano Turi ,  Heinz Vetterli

    发明人： Stefan Stahl ,  Mariano Turi ,  Heinz Vetterli

    IPC分类号： A47J31/44 ,  A23F5/26

    CPC分类号： F25D31/005 ,  A47J31/44

    摘要： A device (1, 10) and method for the production of cooled coffee are provided. The device has a brewing unit (4) for the production of coffee, a cooling assembly (11) and a coffee cooler (14) formed as a heat exchanger. The coffee cooler (14) has at least one first and one second flow path that are connected thermally but not in a fluid-conducting manner. A coolant can flow through the first flow path and the second flow path is connected in a fluid-conducting manner with a coffee outlet (5) of the brewing unit (4). The device also has a fresh-water supply (2) and a fresh-water cooler (13) formed as an additional heat exchanger. The fresh-water cooler (13) has at least one first and one second flow path that are connected thermally but not in a fluid conducting manner and the fresh-water supply (2) is connected in a fluid-conducting manner downstream with the second flow path of the fresh-water cooler (13), further downstream with the first flow path of the coffee cooler (14), and further downstream with the brewing unit (4) and the first flow path of the fresh-water cooler (13) is connected in a fluid-conducting manner with a coolant circuit of the cooling assembly.

    摘要翻译： 提供了一种用于生产冷咖啡的设备（1,10）和方法。 该装置具有用于生产咖啡的冲泡单元（4），形成为热交换器的冷却组件（11）和咖啡冷却器（14）。 咖啡冷却器（14）具有至少一个第一和第二个第二流动路径，其热连接但不以流体传导方式连接。 冷却剂可以流过第一流动路径，并且第二流动路径以流体传导方式连接到冲泡单元（4）的咖啡出口（5）。 该装置还具有作为附加热交换器形成的淡水源（2）和淡水冷却器（13）。 淡水冷却器（13）具有至少一个第一和第二个第二流动路径，它们热连接但不以流体传导方式连接，并且淡水供应源（2）以流体导通的方式与第二个 淡水冷却器（13）的流动路径，进一步下游与咖啡冷却器（14）的第一流动路径连通，并且还具有冲煮单元（4）和淡水冷却器（13）的第一流动路径 ）以流体传导方式与冷却组件的冷却剂回路连接。

    Preparation of 5-cyanovalerates
    8.
    发明授权
    Preparation of 5-cyanovalerates 失效
    5-氰基戊酸酯的制备

    公开(公告)号：US5616773A

    公开(公告)日：1997-04-01

    申请号：US419608

    申请日：1995-04-10

    申请人： Stefan Stahl ,  Wolfgang Harder ,  Arthur Hoehn

    发明人： Stefan Stahl ,  Wolfgang Harder ,  Arthur Hoehn

    IPC分类号： C07C253/30 ,  C07C255/03

    CPC分类号： C07C253/30

    摘要： Preparation of 5-cyanovalerates (I) by the carbonylation of a 1-cyanobutene with carbon monoxide and an alkanol (II) corresponding to the ester radical at an elevated temperature and under superatmospheric pressure in the presence of a cobalt catalyst as well as in the presence of an activating solvent, in which the activating solvent used is an effective amount of a urea of the formula IIIcR.sub.2 N--CO--NR.sub.2 (IIIc)where R is, C.sub.1 -C.sub.6 -alkyl, C.sub.5 -C.sub.7 -cycloalkyl or where 2R groups together form 5-membered to 7-membered rings. The end products I serve as intermediates, chiefly for the preparation of .epsilon.-caprolactam.

    摘要翻译： 在钴催化剂存在下，在升高的温度和超大气压下，通过与一氧化碳和对应于酯基的链烷醇（II）的1-氰基丁腈的羰基化制备5-氰基戊酸酯（I） 活化溶剂的存在，其中使用的活化溶剂是有效量的式IIIcR2N-CO-NR2（IIIc）的脲，其中R是，C1-C6-烷基，C5-C7-环烷基或其中2R基团一起 形成5元至7元环。 最终产品我作为中间体，主要用于制备ε-己内酰胺。

    Wind energy installation
    9.
    发明授权
    Wind energy installation 有权
    风能安装

    公开(公告)号：US09413274B2

    公开(公告)日：2016-08-09

    申请号：US13941658

    申请日：2013-07-15

    申请人： Stefan Stahl

    发明人： Stefan Stahl

    IPC分类号： F03B13/00 ,  H02N1/00 ,  H02N3/00

    CPC分类号： H02N3/00

    摘要： A method for directly converting wind energy into electrical energy is provided. An air stream is ionized and then routed through the electrical field of a collection electrode providing an opposing field for the air ions. The ions are collected on a collection electrode. The electrical potential of the collection electrode is higher than the ionizer, and discharged. A counter-electrode in front of the collection electrode has an electrical potential higher than the collection electrode. Ionized air molecules overcome the electrical field emanating from the counter-electrode via wind power. An apparatus for carrying out the method is provided. An optional retaining electrode, located in front of the ionizer electrode, drives ionized air in the counter-electrode and collection electrode direction. The apparatus is expediently located in a flow channel, with inlet and outlet electrodes at respective ends. A plurality of flow channels combine to form a flow generation module.

    摘要翻译： 提供了将风能直接转换为电能的方法。 气流被电离，然后通过收集电极的电场引导，为空气离子提供相对的场。 离子收集在收集电极上。 收集电极的电位高于电离器，并放电。 在收集电极前面的对电极具有比收集电极高的电位。 电离空气分子克服了通过风力从对电极发出的电场。 提供了一种用于执行该方法的装置。 位于离子电极前面的可选保持电极驱动对电极和收集电极方向的电离空气。 该设备方便地位于流动通道中，其入口和出口电极位于相应的端部。 多个流动通道组合以形成流动产生模块。

    DEVICE FOR THE PROPULSION AND ECCENTRIC BRAKING OF A VEHICLE
    10.
    发明申请
    DEVICE FOR THE PROPULSION AND ECCENTRIC BRAKING OF A VEHICLE 审中-公开
    车辆的推进和偏心制动装置

    公开(公告)号：US20140300078A1

    公开(公告)日：2014-10-09

    申请号：US14239457

    申请日：2011-08-17

    申请人： Kurt Ruffieux ,  Stefan Stahl ,  Andreas Kaiser

    发明人： Kurt Ruffieux ,  Stefan Stahl ,  Andreas Kaiser

    IPC分类号： B62M9/06 ,  B62L5/00

    CPC分类号： B62M9/06 ,  B60T1/062 ,  B62L5/00 ,  B62L5/006 ,  B62M1/36 ,  B62M11/00 ,  B62M11/16 ,  F16H3/005

    摘要： A device for the propulsion and eccentric braking of a vehicle having a pedal drive, which includes a first shaft, a drive mechanism including one or a plurality of chain wheels, belt pulleys or elements of a Cardan drive, a second hollow shaft arranged concentrically about the first shaft mounted into a rear wheel, a freewheel clutch mounted between the first shaft and the drive mechanism, and an optionally engageable reverse gear mechanism mounted between the first and the second shaft. The first and the second shafts are connected to each other in a torsionally rigid manner when the reverse gear mechanism is in the engaged and disengaged state. The freewheel clutch prevents a rotation of the drive mechanism relative to the first shaft in a clockwise direction and is in a freewheeling state when the drive mechanism rotates in a counter-clockwise direction relative to the first shaft.

    摘要翻译： 一种用于具有踏板驱动器的车辆的推进和偏心制动的装置，其包括第一轴，包括一个或多个链轮的驱动机构，皮带轮或卡丹驱动器的元件，第二中空轴同心地布置 安装在后轮中的第一轴，安装在第一轴和驱动机构之间的自由轮离合器，以及安装在第一和第二轴之间的可选择地接合的倒档齿轮机构。 当倒档齿轮机构处于接合和脱离状态时，第一和第二轴以扭转刚性的方式相互连接。 自由轮离合器防止驱动机构相对于第一轴沿顺时针方向旋转，并且当驱动机构相对于第一轴沿逆时针方向旋转时处于续流状态。