公开(公告)号：US20060026705A1

公开(公告)日：2006-02-02

申请号：US11057012

申请日：2005-02-11

申请人： Stephen Allen ,  Anthony Kinney ,  Zhan-Bin Liu ,  Kevin Stecca

发明人： Stephen Allen ,  Anthony Kinney ,  Zhan-Bin Liu ,  Kevin Stecca

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12N9/1205 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

摘要： An isolated polynucleotide encoding a plant cysteine synthase is disclosed. Also disclosed is the construction of a recombinant construct comprising all or part of a coding or non-coding region of the isolated polynucleotide for use in co-suppression or antisense suppression of endogenous nculeic acid sequences encoding polypeptides having cysteine synthase activity. In another aspect, use of this construct to alter levels of cysteine synthase in a plant is disclosed.

摘要翻译： 公开了编码植物半胱氨酸合成酶的分离的多核苷酸。 还公开了构建包含分离的多核苷酸的全部或部分编码区或非编码区的重组构建体，用于共同抑制或反义抑制编码具有半胱氨酸合酶活性的多肽的内源性核酸序列。 在另一方面，公开了使用该构建体来改变植物中半胱氨酸合成酶的水平。

公开(公告)号：US20070237876A1

公开(公告)日：2007-10-11

申请号：US11624777

申请日：2007-01-19

申请人： Anthony Kinney ,  Edgar Cahoon ,  Howard Damude ,  William Hitz ,  Zhan-Bin Liu ,  Charles Kolar

发明人： Anthony Kinney ,  Edgar Cahoon ,  Howard Damude ,  William Hitz ,  Zhan-Bin Liu ,  Charles Kolar

IPC分类号： A01H5/00 ,  A01H5/10 ,  A23D7/00 ,  A23L2/38 ,  C11B11/00

CPC分类号： C12N9/0083 ,  A21D2/165 ,  A23D9/00 ,  A23K20/158 ,  A23K50/40 ,  A23K50/80 ,  A23L11/01 ,  A23L11/03 ,  A23L11/05 ,  A23L11/07 ,  A23L33/12 ,  A23V2002/00 ,  A23V2300/21 ,  C12N9/1029 ,  C12N15/8247 ,  Y02A40/818 ,  A23V2250/1876 ,  A23V2250/1882

摘要： Oilseed plants which have been transformed to produce very long chain polyunsaturated fatty acids, recombinant constructs used in such transformations, methods for producing such fatty acids in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

摘要翻译： 已经转化以产生非常长链多不饱和脂肪酸的油籽植物，用于这种转化的重组构建体，在植物中生产这种脂肪酸的方法，以及从这些转化植物获得的油和种子在多种食物中的应用，以及 描述饲料应用。

公开(公告)号：US20070033673A1

公开(公告)日：2007-02-08

申请号：US11545178

申请日：2006-10-10

申请人： Anthony Kinney ,  Zhan-Bin Liu

发明人： Anthony Kinney ,  Zhan-Bin Liu

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H1/00

CPC分类号： C07H21/04 ,  C12N15/8234

摘要： A seed specific plant annexin and P34 promoters and subfragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

摘要翻译： 描述了种子特异性植物膜联蛋白和P34启动子及其亚片段及其在促进植物中一种或多种异源核酸片段表达的用途。

公开(公告)号：US07985850B2

公开(公告)日：2011-07-26

申请号：US11888497

申请日：2007-07-31

申请人： Stephen Allen ,  Qun Zhu ,  Zhan-Bin Liu

发明人： Stephen Allen ,  Qun Zhu ,  Zhan-Bin Liu

IPC分类号： C12N15/29 ,  C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8218

摘要： This invention relates to an isolated nucleic acid fragment encoding a protein involved in regulation of gene expression. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein involved in regulation of gene expression, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein involved in regulation of gene expression in a transformed host cell.

摘要翻译： 本发明涉及编码参与基因表达调节的蛋白质的分离的核酸片段。 本发明还涉及在正义或反义方向上编码涉及基因表达调控的全部或部分蛋白质的嵌合基因的构建，其中嵌合基因的表达导致生产参与调节的蛋白质水平的改变 在转化的宿主细胞中的基因表达。

公开(公告)号：US10030245B2

公开(公告)日：2018-07-24

申请号：US13427138

申请日：2012-03-22

申请人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

发明人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

IPC分类号： C12N15/82 ,  A01H1/06 ,  C12N9/22

摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

公开(公告)号：US07169969B2

公开(公告)日：2007-01-30

申请号：US11258704

申请日：2005-10-26

申请人： Zhan-Bin Liu ,  Johan M. Stoop

发明人： Zhan-Bin Liu ,  Johan M. Stoop

IPC分类号： C12P7/62 ,  C12P11/00 ,  C07H21/04 ,  A01H1/00

CPC分类号： C12N15/8234 ,  C12N15/8245

摘要： The promoters of a soybean seed maturation protein PM29 and a soybean late-embryogenesis abundant protein LEA3 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

公开(公告)号：US09951346B2

公开(公告)日：2018-04-24

申请号：US14236597

申请日：2012-07-18

申请人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

公开(公告)号：US07129089B2

公开(公告)日：2006-10-31

申请号：US10776889

申请日：2004-02-11

申请人： Anthony J. Kinney ,  Zhan-Bin Liu

发明人： Anthony J. Kinney ,  Zhan-Bin Liu

IPC分类号： C12H15/82 ,  C07H21/04 ,  C12N15/90 ,  A01H5/00

CPC分类号： C07H21/04 ,  C12N15/8234

摘要： A seed specific plant annexin and P34 promoters and subfragments thereof, recombinant expression construct comprising these promoters and their use in driving seed-specific expression of one or more heterologous nucleic acid fragments, such as heterologous nucleic acid fragments encoding reporter constructs or enzymes related to production of fatty acids in plants are described.

摘要翻译： 种子特异性植物膜联蛋白和P34启动子及其亚片段，包含这些启动子的重组表达构建体及其在驱动一个或多个异源核酸片段的种子特异性表达的用途，例如编码与生产相关的报道构建体或酶的异源核酸片段 描述了植物中的脂肪酸。

公开(公告)号：US20060101545A1

公开(公告)日：2006-05-11

申请号：US11258704

申请日：2005-10-26

申请人： Zhan-Bin Liu ,  Johan Stoop

发明人： Zhan-Bin Liu ,  Johan Stoop

IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8234 ,  C12N15/8245

摘要： The promoters of a soybean seed maturation protein PM29 and a soybean late-embryogenesis abundant protein LEA3 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

摘要翻译： 描述了大豆种子成熟蛋白PM29和大豆晚胚发生丰富蛋白LEA3及其片段的启动子及其在促进植物中一种或多种异源核酸片段表达的用途。

公开(公告)号：US08084074B2

公开(公告)日：2011-12-27

申请号：US11673843

申请日：2007-02-12

申请人： Anthony J. Kinney ,  Edgar Benjamin Cahoon ,  Howard Glenn Damude ,  William D. Hitz ,  Zhan-Bin Liu ,  Charles W. Kolar, Jr.

发明人： Anthony J. Kinney ,  Edgar Benjamin Cahoon ,  Howard Glenn Damude ,  William D. Hitz ,  Zhan-Bin Liu ,  Charles W. Kolar, Jr.

IPC分类号： A23D9/00

CPC分类号： A23K20/158 ,  A23L2/52 ,  A23L33/115 ,  A23L33/12

摘要： Oilseed plants which have been transformed to produce at least 8.0% arachidonic acid (ARA) as well as uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

摘要翻译： 已经转化生产至少8.0％花生四烯酸（ARA）的油籽植物以及在各种食品和饲料应用中从这种转化植物获得的油和种子的用途被描述。