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    ANTIBACTERIAL AND PLASMID ELIMINATION AGENTS
    3.
    发明申请
    ANTIBACTERIAL AND PLASMID ELIMINATION AGENTS 审中-公开
    抗生素和血浆消除剂

    公开(公告)号：US20110288008A1

    公开(公告)日：2011-11-24

    申请号：US11758995

    申请日：2007-06-06

    申请人： Kenneth C. Keiler ,  Stephen J. Benkovic

    发明人： Kenneth C. Keiler ,  Stephen J. Benkovic

    IPC分类号： A61K38/12 ,  C07H21/02 ,  C12N1/21 ,  A61P31/04 ,  C12Q1/68 ,  A61K38/08 ,  A61K38/10 ,  C12Q1/18 ,  C40B30/06

    CPC分类号： C12N15/1086 ,  C07K7/06 ,  C07K7/64 ,  C12N15/67 ,  C12Q1/18 ,  G01N2500/10

    摘要： Inhibitors of the tmRNA pathway have antibacterial activity with broad species specificity, including B. anthracis and other pathogens of military and civilian interest. Identified cyclic or linear peptides are further selected by in vivo selection methods, kill bacterial pathogens when added exogenously, and/or eliminate plasmids carrying antibiotic resistance or virulence genes. The molecular target of each cyclic peptide is in the tmRNA pathway and the tmRNA pathway is inhibited in vitro and in vivo by the addition of the peptides.

    摘要翻译： tmRNA途径的抑制剂具有广泛物种特异性的抗菌活性，包括炭疽杆菌和其他军事和民用病原体。 通过体内选择方法进一步选择鉴定的环状或线性肽，当外源性加入时杀死细菌病原体，和/或消除携带抗生素抗性或毒力基因的质粒。 每个环肽的分子靶在tmRNA途径中，并且通过加入肽在体外和体内抑制tmRNA通路。

    METHODS FOR NUCLEIC ACID MANIPULATION
    4.
    发明申请
    METHODS FOR NUCLEIC ACID MANIPULATION 有权
    核酸操作方法

    公开(公告)号：US20100279358A1

    公开(公告)日：2010-11-04

    申请号：US12786070

    申请日：2010-05-24

    申请人： Stephen J. Benkovic ,  Frank Salinas

    发明人： Stephen J. Benkovic ,  Frank Salinas

    IPC分类号： C12P19/34

    CPC分类号： C12P19/34 ,  C12N15/1027 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2521/507 ,  C12Q2521/513 ,  C12Q2527/125

    摘要： A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

    摘要翻译： 描述了用于复制和扩增靶核酸序列的方法。 本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。 用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。 在优选的实施方案中，聚合酶包含聚合酶全酶。 在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白 。

    Incrementally truncated nucleic acids and methods of making same
    8.
    发明授权
    Incrementally truncated nucleic acids and methods of making same 有权
    增量截短的核酸及其制备方法

    公开(公告)号：US07332308B1

    公开(公告)日：2008-02-19

    申请号：US09718465

    申请日：2000-11-15

    申请人： Stephen J. Benkovic ,  Marc Ostermeier ,  Stefan Lutz ,  Andrew E. Nixon

    发明人： Stephen J. Benkovic ,  Marc Ostermeier ,  Stefan Lutz ,  Andrew E. Nixon

    IPC分类号： C12N15/64 ,  C12N5/10 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C12N15/102 ,  C12N15/1027 ,  C12N15/1058

    摘要： A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology, is also described. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides.

    摘要翻译： 描述了利用核酸增量截短的一系列方法来产生多个经修饰的核酸和杂合多肽。 产生给定核酸序列的多个基本上所有可能的单碱基对缺失。 还描述了制造与核酸序列同源性无关的洗牌的增量截短核酸的方法。 这些方法可用于蛋白质工程，蛋白质折叠，蛋白质进化和新型杂合蛋白质和多肽的化学合成。

    Antibodies catalyzing deamidation of proteins
    9.
    发明授权
    Antibodies catalyzing deamidation of proteins 失效
    催化蛋白脱酰胺的抗体

    公开(公告)号：US5439812A

    公开(公告)日：1995-08-08

    申请号：US865216

    申请日：1992-04-08

    申请人： Stephen J. Benkovic ,  Scott D. Taylor ,  Richard A. Gibbs

    发明人： Stephen J. Benkovic ,  Scott D. Taylor ,  Richard A. Gibbs

    IPC分类号： C07K5/072 ,  C12N9/00 ,  C12P21/08 ,  C12N9/82 ,  C12P13/14

    CPC分类号： C07K5/06113 ,  C12N9/0002

    摘要： A hapten is described which may be used to elicit antibodies that catalyze the deamidation of asparginyl-glycyl dipeptides to form two products, the isoaspartyl-glycyl dipeptide and the aspartyl-glycyl dipeptide. A general synthetic scheme is also presented which shows how to modify the hapten to provide specificity for a particular protein in the antibodies that are elicited by the (modified) hapten. Antibodies which catalyze the the deamidation reaction are also described.

    摘要翻译： 描述了半抗原，其可用于引发催化天冬酰高甘油基 - 二酰基脱氨基反应形成两种产物的抗体，即异天竺葵酰甘氨酰二肽和天冬氨酰 - 甘氨酰二肽。 还提供了一般的合成方案，其显示了如何修饰半抗原以提供由（修饰的）半抗原引发的抗体中特定蛋白质的特异性。 还描述了催化脱酰氨反应的抗体。