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公开(公告)号：US5712133A
公开(公告)日：1998-01-27
申请号：US422424
申请日：1995-04-14
申请人： Stephen K. Picataggio , Min Zhang , Christina K. Eddy , Kristine A. Deanda , Mark Finkelstein , Ali Mohagheghi , Mildred M. Newman , James D. McMillan
发明人： Stephen K. Picataggio , Min Zhang , Christina K. Eddy , Kristine A. Deanda , Mark Finkelstein , Ali Mohagheghi , Mildred M. Newman , James D. McMillan
摘要： The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
摘要翻译： 本发明涉及通常不会发酵戊糖并且遗传改变以发酵戊糖以产生乙醇的微生物，以及利用其的发酵方法。 实例包括已经用木糖异构酶，木酮糖激酶，转醛醇酶，转酮酶，L-阿拉伯糖异构酶，L-核糖激酶和L-核酮糖5-磷酸4-差向异构酶的大肠杆菌基因的组合转化的运动发酵单胞菌（Zymomonas mobilis）。 添加的基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素的水解产生的戊糖和葡萄糖以产生乙醇。
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公开(公告)号：US5726053A
公开(公告)日：1998-03-10
申请号：US421996
申请日：1995-04-14
摘要： The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
摘要翻译： 本发明涉及通常不会发酵戊糖并且遗传改变以发酵戊糖以产生乙醇的微生物，以及利用其的发酵方法。 实例包括已经用木糖异构酶，木酮糖激酶，转醛醇酶，转酮酶，L-阿拉伯糖异构酶，L-核糖激酶和L-核酮糖-5-磷酸4-差向异构酶的大肠杆菌基因的组合转化的运动发酵单胞菌（Zymomonas mobilis）。 添加的基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素的水解产生的戊糖和葡萄糖以产生乙醇。
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公开(公告)号：US5514583A
公开(公告)日：1996-05-07
申请号：US228303
申请日：1994-04-15
摘要： The invention relates to microorganisms which normally do not ferment a pentose sugar and which are genetically altered to ferment this pentose to produce ethanol. A representative example is Zymomonas mobilis which has been transformed with E. coli xylose isomerase, xylulokinase, transaldolase and transketolase genes. Expression of the added genes are under the control of Zymomonas mobilis promoters. This newly created microorganism is useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
摘要翻译： 本发明涉及通常不发酵戊糖的遗传改变以发酵该戊糖以产生乙醇的微生物。 代表性实例是已经用大肠杆菌木糖异构酶，木酮糖激酶，转醛醇酶和转酮酶基因转化的运动发酵单胞菌。 添加的基因的表达在运动发酵单胞菌启动子的控制下。 这种新生成的微生物可用于发酵通过半纤维素和纤维素的水解产生的戊糖和葡萄糖以产生乙醇。
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4.发明授权Recombinant lactobacillus for fermentation of xylose to lactic acid and lactate 失效用于将木糖发酵成乳酸和乳酸的重组乳杆菌
公开(公告)号：US5798237A
公开(公告)日：1998-08-25
申请号：US541632
申请日：1995-10-10
CPC分类号： C12N9/1205 , C12N15/746 , C12N9/90 , C12P7/56
摘要： A recombinant Lactobacillus MONT4 is provided which has been genetically engineered with xylose isomerase and xylulokinase genes from Lactobacillus pentosus to impart to the Lactobacillus MONT4 the ability to ferment lignocellulosic biomass containing xylose to lactic acid.
摘要翻译： 提供了重组的乳杆菌MONT4，其已经用来自戊糖乳杆菌的木糖异构酶和木酮糖激酶基因进行了遗传工程改造，以赋予乳酸菌MONT4发酵木质纤维素生物质含木糖至乳酸的能力。
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5.发明授权
公开(公告)号：US5843760A
公开(公告)日：1998-12-01
申请号：US851767
申请日：1997-05-06
摘要： This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.
摘要翻译： 本发明涉及通常不发酵戊糖的单一微生物，其经遗传改变以发酵戊糖，木糖和阿拉伯糖以产生乙醇，以及利用该微生物的发酵方法。 实例包括用木糖异构酶的大肠杆菌基因，木酮糖激酶，L-阿拉伯糖异构酶，L-核糖激酶，L-核酮糖-5-磷酸4-差向异构酶，转醛醇酶和转酮酶的组合转化的运动发酵单胞菌。 添加基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素或淀粉的水解产生的葡萄糖，木糖和阿拉伯糖，以产生乙醇。
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公开(公告)号：US07932077B2
公开(公告)日：2011-04-26
申请号：US11265761
申请日：2005-11-02
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
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公开(公告)号：US20090233346A1
公开(公告)日：2009-09-17
申请号：US12469467
申请日：2009-05-20
CPC分类号： C12N15/815 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.
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公开(公告)号：US07550286B2
公开(公告)日：2009-06-23
申请号：US11264737
申请日：2005-11-01
申请人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
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9.发明申请GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST 审中-公开甘油三磷酸脱氢酶和磷脂酰肌醇六磷酸酯促进剂，用于在OLEAGOOY YEAST中进行基因表达公开(公告)号：US20080220474A1
公开(公告)日：2008-09-11
申请号：US11773453
申请日：2007-07-31
IPC分类号： C12P21/06
CPC分类号： C12P7/6427 , C12N9/0008 , C12N9/90 , C12N15/815 , C12P7/6472
摘要： The promoter regions associated with the Yarrowia lipolytica glyceraldehyde-3-phosphate dehydrogenase (gpd) and phosphoglycerate mutase (gpm) genes have been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the invention have been shown to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.
摘要翻译： 已经发现与解脂耶氏酵母甘油醛-3-磷酸脱氢酶（gpd）和磷酸甘油酸变位酶（gpm）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已经显示本发明的启动子区域驱动参与产生ω-3和ω-6脂肪酸的基因的高水平表达。
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10.发明授权公开(公告)号：US07125672B2
公开(公告)日：2006-10-24
申请号：US10840478
申请日：2004-05-06
CPC分类号： C12N9/0071 , C12N9/1029
摘要： The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.
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