利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

10 条记录 (耗时 0.035 秒)

    Pichia pastoris formate dehydrogenase and uses therefor
    1.
    发明授权
    Pichia pastoris formate dehydrogenase and uses therefor 有权
    毕赤酵母甲酸脱氢酶，用于此

    公开(公告)号：US07087418B2

    公开(公告)日：2006-08-08

    申请号：US10320300

    申请日：2002-12-16

    申请人： Steven L. Goldberg ,  Paul M. Cino ,  Ramesh N. Patel ,  Venkata B. Nanduri ,  Robert M. Johnston

    发明人： Steven L. Goldberg ,  Paul M. Cino ,  Ramesh N. Patel ,  Venkata B. Nanduri ,  Robert M. Johnston

    IPC分类号： C12N9/04 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/0004 ,  C12Q1/26 ,  G01N2333/04

    摘要： This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及催化甲酸氧化成二氧化碳并同时将烟酰胺腺嘌呤二核苷酸（NAD +）还原为还原形式（NADH）的重组巴斯德毕赤酵母甲酸脱氢酶（FDH）酶。 还涉及编码巴斯德毕赤酵母多肽的分离的核酸及其片段和变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的重组巴斯德毕赤酵母FDH多肽及其片段和变体，以及特异性结合巴斯德毕赤酵母多肽，片段或变体的抗体。 本发明还涉及获得分离的巴斯德毕赤酵母FDH核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用巴斯德毕赤酵母FDH的方法。

    Gluconobacter oxydans 2-ketoreductase enzyme and applications thereof
    2.
    发明授权
    Gluconobacter oxydans 2-ketoreductase enzyme and applications thereof 失效
    氧化葡糖杆菌2-酮还原酶及其应用

    公开(公告)号：US06929935B2

    公开(公告)日：2005-08-16

    申请号：US10320104

    申请日：2002-12-16

    申请人： Venkata B. Nanduri ,  Robert M. Johnston ,  Steven L. Goldberg ,  Paul M. Cino ,  Ramesh N. Patel

    发明人： Venkata B. Nanduri ,  Robert M. Johnston ,  Steven L. Goldberg ,  Paul M. Cino ,  Ramesh N. Patel

    IPC分类号： C12N1/21 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/32 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/0004 ,  C12Q1/26 ,  C12Q1/32 ,  G01N2333/04

    摘要： This invention relates to an novel Gluconobacter oxydans 2-ketoreductase useful for the synthesis of chiral alcohols. Also related are isolated nucleic acids encoding G. oxydans 2-ketoreductase enzymes, and enzyme fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated G. oxydans 2-ketoreductase polypeptides, and fragments and variants thereof, and antibodies that specifically bind to G. oxydans 2-ketoreductase polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated G. oxydans 2-ketoreductase nucleic acids, polypeptides, and antibodies, and methods of using G. oxydans 2-ketoreductase in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及可用于合成手性醇的新型氧化葡糖杆菌2-酮还原酶。 还涉及编码G.oxydans 2-酮还原酶的分离的核酸及其酶片段及其变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的G.oxydans 2-酮还原酶多肽及其片段和变体，以及特异性结合G.oxydans 2-酮还原酶多肽，片段或变体的抗体。 本发明还涉及获得分离的G.oxydans 2-酮还原酶核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用G.oxydans 2-酮还原酶的方法。

    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor
    3.
    发明授权
    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor 失效
    N-苄氧羰基（N-CBZ） - 保护酶，并用于此

    公开(公告)号：US07119188B2

    公开(公告)日：2006-10-10

    申请号：US10453418

    申请日：2003-06-03

    申请人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    发明人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    IPC分类号： C07H21/04 ,  C12N9/14 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/78

    摘要： This invention relates to isolated or recombinant N-carbobenzyloxy-deprotecting enzyme polypeptides, and variants and fragments thereof, that catalyze the removal of carbobenzyloxy from carbobenzyloxy-protected amino acids and alcohols. Also related are isolated nucleic acids encoding N-carbobenzyloxy-deprotecting enzyme polypeptides, and variants, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using the polypeptides in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及分离或重组的N-苄氧羰基脱保护酶多肽及其变体和片段，其催化从苄氧羰基保护的氨基酸和醇中除去苄氧羰基。 还涉及编码N-苄氧羰基脱保护酶多肽及其变体及其片段的分离的核酸以及包含这些核酸的载体和宿主细胞。 本发明还涉及获得分离的核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用该多肽的方法。

    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor
    5.
    发明授权
    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor 失效
    N-苄氧羰基（N-CBZ） - 保护酶，并用于此

    公开(公告)号：US07696328B2

    公开(公告)日：2010-04-13

    申请号：US12150544

    申请日：2008-04-28

    申请人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    发明人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    IPC分类号： C07K16/00 ,  C07K16/40 ,  C07K16/46 ,  C07K14/00 ,  C07K5/00

    CPC分类号： C12N9/78

    摘要： This invention relates to isolated or recombinant N-carbobenzyloxy-deprotecting enzyme polypeptides that catalyze the removal of carbobenzyloxy from carbobenzyloxy-protected amino acids and alcohols. Also related are isolated nucleic acids encoding N-carbobenzyloxy-deprotecting enzyme polypeptides thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using the polypeptides in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及催化从苄氧羰基保护的氨基酸和醇中除去苄氧羰基的分离的或重组的N-苄氧羰基脱保护酶多肽。 还涉及编码其N-苄氧羰基脱保护酶多肽的分离的核酸，以及包含这些核酸的载体和宿主细胞。 本发明还涉及获得分离的核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用该多肽的方法。

    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor
    6.
    发明申请
    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor 失效
    N-苄氧羰基（N-CBZ） - 保护酶，并用于此

    公开(公告)号：US20090104670A1

    公开(公告)日：2009-04-23

    申请号：US12150544

    申请日：2008-04-28

    申请人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    发明人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    IPC分类号： C12P13/00 ,  C07K16/18 ,  C12P7/02

    CPC分类号： C12N9/78

    摘要： This invention relates to isolated or recombinant N-carbobenzyloxy-deprotecting enzyme polypeptides that catalyze the removal of carbobenzyloxy from carbobenzyloxy-protected amino acids and alcohols. Also related are isolated nucleic acids encoding N-carbobenzyloxy-deprotecting enzyme polypeptides thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using the polypeptides in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及催化从苄氧羰基保护的氨基酸和醇中除去苄氧羰基的分离的或重组的N-苄氧羰基脱保护酶多肽。 还涉及编码其N-苄氧羰基脱保护酶多肽的分离的核酸，以及包含这些核酸的载体和宿主细胞。 本发明还涉及获得分离的核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用该多肽的方法。

    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme
    7.
    发明授权
    N-carbobenzyloxy (N-CBZ)-deprotecting enzyme 失效
    N-苄氧羰基（N-CBZ） - 保护酶

    公开(公告)号：US07416876B2

    公开(公告)日：2008-08-26

    申请号：US11502704

    申请日：2006-08-10

    申请人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    发明人： Venkata B. Nanduri ,  Ramesh N. Patel ,  Steven L. Goldberg ,  Robert M. Johnston

    IPC分类号： C12N9/16 ,  C12P21/06

    CPC分类号： C12N9/78

    摘要： This invention relates to isolated or recombinant N-CBZ-deprotecting enzyme polypeptides that catalyze the removal of CBZ from CBZ-protected amino acids and alcohols, and variants, modifications, and fragments thereof. Also related are isolated nucleic acids encoding N-CBZ-deprotecting enzyme polypeptides, and variants, modifications, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using polypeptides in various reactions for industrial or pharmaceutical applications.

    摘要翻译： 本发明涉及分离或重组的N-CBZ脱保护酶多肽，其催化从CBZ保护的氨基酸和醇及其变体，修饰和片段中除去CBZ。 还涉及编码N-CBZ-脱保护酶多肽的分离的核酸及其变体，修饰和片段，以及包含这些核酸的载体和宿主细胞。 本发明还涉及获得分离的核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用多肽的方法。