公开(公告)号：US08946512B2

公开(公告)日：2015-02-03

申请号：US13060881

申请日：2009-08-31

申请人： Valerie Frankard ,  Andry Andriankaja ,  Yves Hatzfeld ,  Marieke Louwers ,  Steven Vandenabeele ,  Aurine Verkest ,  Geert De Jaeger ,  Dirk Inzé

发明人： Valerie Frankard ,  Andry Andriankaja ,  Yves Hatzfeld ,  Marieke Louwers ,  Steven Vandenabeele ,  Aurine Verkest ,  Geert De Jaeger ,  Dirk Inzé

IPC分类号： C12N15/82 ,  C12N5/04 ,  C12N15/00 ,  C07H21/04 ,  A01H5/00

CPC分类号： C12N15/8261 ,  C12N15/8216 ,  Y02A40/146

摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of one or more nucleic acid(s) encoding at least two iSYT (interactor of SYT-synovial sarcoma translocation-) polypeptides. The present invention also concerns plants having modulated expression of a nucleic acid encoding at least two iSYT polypeptides, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides hitherto unknown nucleic acids encoding at least two iSYT polypeptides, and constructs comprising the same, useful in performing the methods of the invention. The invention also provides constructs useful in the methods of the invention. Furthermore the present invention also relates to an iSYT-based protein complex. It further relates to the use of the complex to promote plant growth, and to a method for stimulating the complex formation, by overexpressing at least two members of the complex.

摘要翻译： 本发明一般涉及分子生物学领域，并且涉及一种通过调节植物中编码至少两种iSYT（SYT滑膜肉瘤的相互作用者）的一种或多种核酸的表达来增强植物中产量相关性状的方法 易位 - ）多肽。 本发明还涉及具有编码至少两种iSYT多肽的核酸的调节表达的植物，该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 本发明还提供了迄今未知的编码至少两种iSYT多肽的核酸，以及包含其的构建体，其可用于实施本发明的方法。 本发明还提供了可用于本发明方法的构建体。 此外，本发明还涉及基于iSYT的蛋白质复合物。 它还涉及复合物促进植物生长的用途，以及通过过表达复合物的至少两个成员来刺激复合物形成的方法。

公开(公告)号：US20140298545A1

公开(公告)日：2014-10-02

申请号：US14233269

申请日：2012-07-12

申请人： Steven Vandenabeele ,  Andry Andriankaja

发明人： Steven Vandenabeele ,  Andry Andriankaja

IPC分类号： C12N15/82 ,  C07K14/415 ,  A01H5/10 ,  A01H5/00

CPC分类号： C12N15/8261 ,  A01H5/00 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8273 ,  Y02A40/146

摘要： The present invention provides a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an F-box Skp2-like polypeptide, or a DUF584 polypeptide. The present invention also provides plants having modulated expression of a nucleic acid encoding an F-box Skp2-like polypeptide, or a DUF584 polypeptide, which plants have enhanced yield-related traits compared to control plants.

摘要翻译： 本发明提供了一种通过调节植物中编码F-box Skp2样多肽或DUF584多肽的核酸表达来增强植物中产量相关性状的方法。 本发明还提供具有编码F盒Skp2样多肽或DUF584多肽的核酸的调节表达的植物，与对照植物相比，植物具有增强的产量相关性状。

公开(公告)号：US20110214207A1

公开(公告)日：2011-09-01

申请号：US13060881

申请日：2009-08-31

申请人： Valerie Frankard ,  Andry Andriankaja ,  Yves Hatzfeld ,  Marieke Louwers ,  Steven Vandenabeele ,  Aurine Verkest ,  Geert De Jaeger ,  Dirk Inze

发明人： Valerie Frankard ,  Andry Andriankaja ,  Yves Hatzfeld ,  Marieke Louwers ,  Steven Vandenabeele ,  Aurine Verkest ,  Geert De Jaeger ,  Dirk Inze

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/10 ,  C12N15/87 ,  A01H5/04 ,  A01H5/00

CPC分类号： C12N15/8261 ,  C12N15/8216 ,  Y02A40/146

摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of one or more nucleic acid(s) encoding at least two iSYT (inter-actor of SYT-synovial sarcoma translocation-) polypeptides. The present invention also concerns plants having modulated expression of a nucleic acid encoding at least two iSYT polypeptides, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides hitherto unknown nucleic acids encoding at least two iSYT polypeptides, and constructs comprising the same, useful in performing the methods of the invention. The invention also provides constructs useful in the methods of the invention. Furthermore the present invention also relates to an iSYT-based protein complex. It further relates to the use of the complex to promote plant growth, and to a method for stimulating the complex formation, by overexpressing at least two members of the complex.

摘要翻译： 本发明一般涉及分子生物学领域，并且涉及通过调节植物中编码至少两种iSYT（SYT-1的相互作用者）的一种或多种核酸的表达来增强植物中产量相关性状的方法， 滑膜肉瘤易位 - ）多肽。 本发明还涉及具有编码至少两种iSYT多肽的核酸的调节表达的植物，该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 本发明还提供了迄今未知的编码至少两种iSYT多肽的核酸，以及包含其的构建体，其可用于实施本发明的方法。 本发明还提供了可用于本发明方法的构建体。 此外，本发明还涉及基于iSYT的蛋白质复合物。 它还涉及复合物促进植物生长的用途，以及通过过表达复合物的至少两个成员来刺激复合物形成的方法。

公开(公告)号：US20150322449A1

公开(公告)日：2015-11-12

申请号：US14344923

申请日：2012-09-06

申请人： Valerie Frankard ,  Christophe Reuzeau ,  Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Steven Vandenabeele

发明人： Valerie Frankard ,  Christophe Reuzeau ,  Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Steven Vandenabeele

IPC分类号： C12N15/82 ,  C07K14/415 ,  A01G1/00 ,  C12N9/08

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N9/0065 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y111/01009 ,  C12Y111/01012 ,  Y02A40/146

摘要： Provided are methods for enhancing yield-related traits in plants by modulating expression of a nucleic acid encoding an ELM2-related polypeptide, or a WRKY-related polypeptide, or an EMG1-like polypeptide, or a GPx-related polypeptide in a plant. Also provided are plants having modulated expression of a nucleic acid encoding an ELM2-related polypeptide, or a WRKY-related polypeptide, or an EMG1-like polypeptide, or a GPx-related polypeptide, which plants have enhanced yield-related traits relative to control plants. Further provided are constructs comprising a nucleic acid encoding an ELM2-related polypeptide, or a WRKY-related polypeptide, or an EMG1-like polypeptide, or a GPx-related polypeptide, useful in carrying out the methods of the invention.

摘要翻译： 提供了通过调节植物中编码ELM2相关多肽或WRKY相关多肽或EMG1样多肽或GPx相关多肽的核酸的表达来增强植物中产量相关性状的方法。 还提供了具有编码与ELM2相关的多肽或WRKY相关多肽或EMG1样多肽或GPx相关多肽的核酸的调节表达的植物，该植物相对于对照具有增强的产量相关性状 植物。 还提供了包含编码与实施本发明的方法有关的ELM2相关多肽或WRKY相关多肽或EMG1样多肽或GPx相关多肽的核酸的构建体。

公开(公告)号：US20110321197A1

公开(公告)日：2011-12-29

申请号：US13125433

申请日：2009-10-02

申请人： Hardy Schön ,  Oliver Thimm ,  Gerhard Ritte ,  Oliver Bläsing ,  Koen Bruynseels ,  Yves Hatzfeld ,  Valerie Frankard ,  Ana Isabel Sanz Molinero ,  Christophe Reuzeau ,  Steven Vandenabeele

发明人： Hardy Schön ,  Oliver Thimm ,  Gerhard Ritte ,  Oliver Bläsing ,  Koen Bruynseels ,  Yves Hatzfeld ,  Valerie Frankard ,  Ana Isabel Sanz Molinero ,  Christophe Reuzeau ,  Steven Vandenabeele

IPC分类号： A01H1/06 ,  C12N15/63 ,  C12P21/00 ,  C12N9/88 ,  C12N9/12 ,  C12N9/10 ,  C12N9/00 ,  C12N9/04 ,  C12N9/16 ,  C12N9/22 ,  C12N9/90 ,  C07K2/00 ,  C07K16/40 ,  C07K16/14 ,  C07K16/12 ,  C12N5/10 ,  A01H5/00 ,  C12Q1/68 ,  G01N33/53 ,  C12Q1/02 ,  C12Q1/28 ,  C12Q1/32 ,  C12Q1/48 ,  C12Q1/44 ,  C12Q1/42 ,  C08B30/04 ,  A01C14/00 ,  C07H21/00

CPC分类号： C12N15/8273 ,  C12N15/8261 ,  Y02A40/146

摘要： A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities selected from the group consisting of 17.6 kDa class I heat shock protein, 26.5 kDa class I small heat shock protein, 26S protease subunit, 2-Cys peroxiredoxin, 3-dehydroquinate synthase, 5-keto-D-gluconate-5-reductase, asparagine synthetase A, aspartate 1-decar-boxylase precursor, ATP-dependent RNA helicase, B0567-protein, B1088-protein, B1289-protein, B2940-protein, calnexin homolog, CDS5399-protein, chromatin structure-remodeling complex protein, D-amino acid dehydrogenase, D-arabinono-1,4-lac-tone oxidase, Delta 1-pyrroline-5-carboxylate reductase, glycine cleavage complex lipoylprotein, ketodeoxygluconokinase, lipoyl synthase, low-molecular-weight heat-shock protein, Microsomal cytochrome b reductase, mitochondrial ribosomal protein, mitotic check point protein, monodehydroascorbate reductase, paraquat-inducible protein B, phosphatase, Phosphoglucosamine mutase, protein disaggregation chaperone, protein kinase, pyruvate decarboxylase, recA family protein, rhodanese-related sulfurtransferase, ribonuclease P protein component, ribosome modulation factor, sensory histidine kinase, serine hydroxymethyltransferase, SLL1280-protein, SLL1797-protein, small membrane lipoprotein, Small nucleolar ribonucleoprotein complex subunit, Sulfatase, transcription initiation factor subunit, tretraspanin, tRNA ligase, xyloglucan galactosyltransferase, YKL130C-protein, YLR443W-protein, YML096W-protein, and zinc finger family protein-activity.

摘要翻译： 与相应的野生型植物相比，生产具有增加的产量的植物的方法，其中所述方法至少包括以下步骤：在植物或其部分中增加或产生一种或多种选自17.6kDa类的活性 I热休克蛋白，26.5 kDa I类小热休克蛋白，26S蛋白酶亚基，2-Cys过氧化还原蛋白，3-脱氢秋水仙素合成酶，5-酮-D-葡萄糖酸-5-还原酶，天冬酰胺合成酶A，天冬氨酸1-脱羧基酶 前体，ATP依赖性RNA解旋酶，B0567-蛋白，B1088-蛋白，B1289-蛋白，B2940蛋白，钙粘蛋白同源物，CDS5399-蛋白，染色质结构重塑复合蛋白，D-氨基酸脱氢酶，D-阿拉伯聚糖-1， 4-lac-色素氧化酶，1α-吡咯啉-5-羧酸还原酶，甘氨酸裂解复合物脂酰蛋白，酮脱氧葡萄糖激酶，脂酰基合成酶，低分子量热休克蛋白，微粒体细胞色素b还原酶，线粒体核糖体蛋白，有丝分裂检测点 nt蛋白，单脱氢抗坏血酸还原酶，百草枯诱导蛋白B，磷酸酶，磷酸葡糖胺变位酶，蛋白质分解伴侣蛋白，蛋白激酶，丙酮酸脱羧酶，recA家族蛋白，硫酸相关硫转移酶，核糖核酸酶P蛋白组分，核糖体调节因子，感觉组氨酸激酶，丝氨酸 羟基甲基转移酶，SLL1280蛋白，SLL1797蛋白，小膜脂蛋白，小核仁核糖核蛋白复合子亚基，硫酸酯酶，转录起始因子亚基，tretraspanin，tRNA连接酶，木葡聚糖半乳糖基转移酶，YKL130C-蛋白，YLR443W蛋白，YML096W蛋白和锌指 家庭蛋白质活性。

公开(公告)号：US20140250548A1

公开(公告)日：2014-09-04

申请号：US14111876

申请日：2012-04-13

申请人： Christophe Reuzeau ,  Steven Vandenabeele

发明人： Christophe Reuzeau ,  Steven Vandenabeele

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  C12N15/8223 ,  Y02A40/146

摘要： Provided is a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an NEMTOP6 polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding an NEMTOP6 polypeptide, which plants have enhanced yield-related traits compared with control plants. Also provided are NEMTOP6-encoding nucleic acids, and constructs comprising the same, useful in enhancing yield-related traits in plants.

摘要翻译： 提供了一种通过调节植物中编码NEMTOP6多肽的核酸的表达来增强植物中产量相关性状的方法。 还提供了具有调节表达编码NEMTOP6多肽的核酸的植物，与对照植物相比，该植物具有增强的产量相关性状。 还提供了编码NEMTOP6的核酸和包含其的构建体，其可用于增强植物中的产量相关性状。

公开(公告)号：US20100132071A1

公开(公告)日：2010-05-27

申请号：US12598055

申请日：2008-05-02

申请人： Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Amber Shirley ,  Lalitree Darnielle ,  Valerie Frankard ,  Steven Vandenabeele ,  Bryan McKersie

发明人： Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Amber Shirley ,  Lalitree Darnielle ,  Valerie Frankard ,  Steven Vandenabeele ,  Bryan McKersie

IPC分类号： C12N15/82 ,  C12N15/29 ,  C07K14/415 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N9/0071 ,  C12N9/12 ,  C12N15/8216 ,  C12N15/827 ,  C12N15/8273 ,  C12Y114/11 ,  C12Y207/11001 ,  Y02A40/146

摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits and/or improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth Regulating Protein). The GRP is selected from a LOB-domain comprising protein (LOB: Lateral Organ Boundaries), herein abbreviated as LBD polypeptide, a JMJC (JUMONJI-C) polypeptide, a CKI (Casein Kinase I) polypeptide, a bHLH11-like (basic Helix-Loop-Helix 11) protein, a plant homeodomain finger-homeodomain (PHDf-HD) polypeptide, an ASR (abscisic acid-, stress-, and ripening-induced) polypeptide and/or a Squamosa promoter binding protein-like 1 1 (SPL11) transcription factor polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides novel GRP nucleic acids and GRP polypeptides as well as constructs useful in the methods of the invention.

摘要翻译： 本发明一般涉及分子生物学领域，涉及通过调节植物中编码GRP（生长调节蛋白）的核酸的表达来增强产量相关性状和/或改善各种植物生长特性的方法。 GRP选自包含蛋白质（LOB：横向器官界限）的LOB结构域，本文缩写为LBD多肽，JMJC（JUMONJI-C）多肽，CKI（酪蛋白激酶I）多肽，bHLH11样（碱性螺旋 -Loop-Helix 11）蛋白，植物同源异型域同源结构域（PHDf-HD）多肽，ASR（脱落酸，胁迫和成熟诱导的）多肽和/或Squamosa启动子结合蛋白样11 SPL11）转录因子多肽。 本发明还涉及具有调节GRP核酸表达的植物，该植物相对于相应的野生型植物或其它对照植物具有改善的生长特性。 本发明还提供新颖的GRP核酸和GRP多肽以及可用于本发明方法的构建体。

公开(公告)号：US20130160165A1

公开(公告)日：2013-06-20

申请号：US13805857

申请日：2011-06-21

申请人： Christophe Reuzeau ,  Steven Vandenabeele ,  Valerie Frankard

发明人： Christophe Reuzeau ,  Steven Vandenabeele ,  Valerie Frankard

IPC分类号： C12N15/82

CPC分类号： C12N15/8262 ,  C07K14/415 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  Y02A40/146

摘要： The present invention relates generally to the field of molecular biology and to the methods for enhancing various economically important yield-related traits in plants. More specifically, the present invention relates to a method for enhancing yield-related traits in plants by modulating expression of a nucleic acid encoding an OsRSZ33 RRM polypeptide or a growth-related protein (GRP) having at least 25% amino acid sequence identity to SEQ ID NO:251 or a ZPR polypeptide. The present invention also relates to plants having modulated expression of a nucleic acid encoding an OsRSZ33 RRM polypeptide or a growth-related polypeptide as defined herein or a ZPR polypeptide. Such plants have enhanced yield-related traits relative to controls. The invention also provides hitherto unknown OsRSZ33 RRM-encoding nucleic acids or GRP-encoding nucleic acids or a ZPR polypeptide, and constructs comprising the same, which are useful in performing the methods of the invention.

摘要翻译： 本发明一般涉及分子生物学领域和用于增强植物中各种经济上重要的产量相关性状的方法。 更具体地，本发明涉及一种通过调节编码OsRSZ33 RRM多肽或与SEQ ID不超过25％氨基酸序列同一性的生长相关蛋白（GRP）的核酸的表达来增强植物中产量相关性状的方法。 SEQ ID NO：251或ZPR多肽。 本发明还涉及具有编码如本文定义的OsRSZ33 RRM多肽或生长相关多肽或ZPR多肽的核酸的调节表达的植物。 相对于对照，这些植物具有增强的产量相关性状。 本发明还提供迄今为止未知的OsRSZ33RRM编码核酸或GRP编码核酸或ZPR多肽，以及包含其的构建体，其可用于实施本发明的方法。

公开(公告)号：US20130125264A1

公开(公告)日：2013-05-16

申请号：US13810267

申请日：2011-07-15

申请人： Valerie Frankard ,  Steven Vandenabeele

发明人： Valerie Frankard ,  Steven Vandenabeele

IPC分类号： C12N15/82

CPC分类号： C12N15/8273 ,  C12N15/8261 ,  Y02A40/146

摘要： Nucleic acids and the encoded embryonic flower 2 (EMF2) polypeptides or Ubiquitin C-terminal Hydrolase 1 (UCH1-like) polypeptides are provided. A method of enhancing yield-related traits in plants by modulating expression of nucleic acids encoding EMF2 polypeptides or UCH1-like polypeptides is provided. Plants with modulated expression of the nucleic acids encoding EMF2 polypeptides or UCH1-like polypeptides have enhanced yield-related traits relative to control plants.

摘要翻译： 提供核酸和编码的胚胎花2（EMF2）多肽或泛素C-端水解酶1（UCH1样多肽）。 提供了一种通过调节编码EMF2多肽或UCH1样多肽的核酸表达来增强植物中产量相关性状的方法。 具有编码EMF2多肽或UCH1样多肽的核酸调节表达的植物相对于对照植物具有增强的产量相关性状。

公开(公告)号：US20110209241A1

公开(公告)日：2011-08-25

申请号：US12863800

申请日：2009-01-23

申请人： Yves Hatzfeld ,  Valerie Frankard ,  Christophe Reuzeau ,  Ana Isabel Sanz Molinero ,  Steven Vandenabeele

发明人： Yves Hatzfeld ,  Valerie Frankard ,  Christophe Reuzeau ,  Ana Isabel Sanz Molinero ,  Steven Vandenabeele

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10 ,  A01H5/12 ,  A01H5/10 ,  C07H21/04

CPC分类号： C12N15/8216 ,  C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a PRE-like (Paclobutrazol REsistance) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a PRE-like polypeptide, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding an SCE1 (SUMO Conjugating Enzyme 1). The present invention also concerns plants having modulated expression of a nucleic acid encoding an SCE1, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides hitherto unknown SCE1-encoding nucleic acids, and constructs comprising the same, useful in performing the methods of the invention. In yet another embodiment, M the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield related-traits by modulating expression in a plant of a nucleic acid encoding a YEF1 (Yield Enhancing Factor 1). The present invention also concerns plants having modulated expression of a nucleic acid encoding a YEF1, which plants have enhanced yield related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In yet another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding a subgroup III glutaredoxin (Grx). The present invention also concerns plants having modulated expression of a nucleic acid encoding a subgroup III Grx, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In a further embodiment, the present invention relates generally to the field of molecular biology and concerns a method for altering the ratio of roots to shoots in plants by modulating expression in a plant of a nucleic acid encoding a Sister of FT protein or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a Sister of FT protein or a homologue thereof, which plants have altered root to shoot ratio relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.