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14 条记录 (耗时 0.019 秒)

    Genome minimization by tn5-coupled cre/loxP excision system
    1.
    发明授权
    Genome minimization by tn5-coupled cre/loxP excision system 失效
    通过tn5耦合的cre / loxP切除系统进行基因组最小化

    公开(公告)号：US07745218B2

    公开(公告)日：2010-06-29

    申请号：US10505328

    申请日：2002-10-31

    申请人： Sun-Chang Kim ,  Byung-Jo Yu

    发明人： Sun-Chang Kim ,  Byung-Jo Yu

    IPC分类号： C12N15/00 ,  C12N15/70 ,  C12N15/74 ,  C07H21/04

    CPC分类号： C12N15/63 ,  C12N15/90

    摘要： Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.

    摘要翻译： 公开了一种通过Cre表达载体使用转座子和Cre / loxP位点特异性重组开发新型菌株缺失特定染色体位点的方法，其中转座子包含选择性标记和loxP位点。 该方法包括以下步骤：（1）制备包含可选择标记和loxP位点的转座子; （2）将转座子插入微生物染色体的任选位置，并确定插入位点; （3）将包含不同选择标记的两个转座子整合到一条染色体上; （4）通过将Cre表达载体导入步骤（3）的染色体中，删除两个lox位点之间的染色体位点; （5）重复步骤（3和4），突变体删除一部分染色体，逐渐缩短突变体的染色体。

    Construction of novel strains containing minimizing genome by tn5-coupled cre/loxp excision system
    2.
    发明申请
    Construction of novel strains containing minimizing genome by tn5-coupled cre/loxp excision system 失效
    通过tn5偶联的cre / loxp切除系统构建含有最小化基因组的新菌株

    公开(公告)号：US20090104682A1

    公开(公告)日：2009-04-23

    申请号：US10505328

    申请日：2002-10-31

    申请人： Sun-Chang Kim ,  Byung-Jo Yu

    发明人： Sun-Chang Kim ,  Byung-Jo Yu

    IPC分类号： C12N7/01 ,  C12N15/63

    CPC分类号： C12N15/63 ,  C12N15/90

    摘要： Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.

    摘要翻译： 公开了一种通过Cre表达载体使用转座子和Cre / loxP位点特异性重组来开发新的菌株缺失特定染色体位点的方法，其中转座子包含选择性标记和loxP位点。 该方法包括以下步骤：（1）制备包含可选择标记和loxP位点的转座子; （2）将转座子插入微生物染色体的任选位置，并确定插入位点; （3）将包含不同选择标记的两个转座子整合到一条染色体上; （4）通过将Cre表达载体导入步骤（3）的染色体中，删除两个lox位点之间的染色体位点; （5）重复步骤（3和4），突变体删除一部分染色体，逐渐缩短突变体的染色体。

    Linear Dna Fragment For Markerless Deletion, Novel Strain Having Inhibited Formation Of Biofilm And Preparation Method Thereof
    3.
    发明申请
    Linear Dna Fragment For Markerless Deletion, Novel Strain Having Inhibited Formation Of Biofilm And Preparation Method Thereof 审中-公开
    用于无标记缺失的线性Dna片段，具有抑制形成生物膜的新型菌株及其制备方法

    公开(公告)号：US20070287180A1

    公开(公告)日：2007-12-13

    申请号：US11574484

    申请日：2005-09-01

    申请人： Sun-Chang Kim ,  Choong-Hoon Lee ,  Bo-Ram Lim ,  Bong-Hyun Sung ,  Byung-Jo Yu ,  Won-Sik Lee ,  Jun-Hyoung Lee ,  Sang-Hee Lee

    发明人： Sun-Chang Kim ,  Choong-Hoon Lee ,  Bo-Ram Lim ,  Bong-Hyun Sung ,  Byung-Jo Yu ,  Won-Sik Lee ,  Jun-Hyoung Lee ,  Sang-Hee Lee

    IPC分类号： C12N15/70 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12N15/70 ,  C12N15/52

    摘要： The present invention relates to Escherichia coli variants that have increased antibiotics susceptibility, diffusion efficiency, and transformation efficiency. The variants can minimize the problems caused by biofilm formation such as increased resistance to antibiotics, decreased solute diffusion efficiency, and lowered transformation efficiency. According to the present invention, when selecting genetically-modified E. coli variants, not only a lesser amount of antibiotics is required when selecting desirable variants, but also the reduction of selection efficiency caused by biofilm formation by strains other than the variants to be selected, thus decreasing exhibiting resistance to antibiotics, can be avoided. Additionally, in the process of materials production, the amount of secreted products could be increased due to the increased solute diffusion efficiency. Furthermore, the increased transformation efficiency makes the mass production of useful materials easier.

    摘要翻译： 本发明涉及具有增加的抗生素敏感性，扩散效率和转化效率的大肠杆菌变体。 这些变体可以最小化由生物膜形成引起的问题，例如抗生素抗性增加，溶质扩散效率降低，转化效率降低。 根据本发明，当选择遗传修饰的大肠杆菌变体时，不仅在选择期望的变体时需要较少量的抗生素，而且还降低由选择的变体以外的菌株生物膜形成引起的选择效率 ，从而减少对抗生素的抗性。 另外，在材料生产过程中，由于溶质扩散效率的提高，分泌产物的量可能会增加。 此外，提高的转换效率使得有用材料的大量生产更容易。

    Use of antimicrobial peptides in regeneration of skin cells
    7.
    发明授权
    Use of antimicrobial peptides in regeneration of skin cells 有权
    抗菌肽在皮肤细胞再生中的应用

    公开(公告)号：US08871716B2

    公开(公告)日：2014-10-28

    申请号：US13095763

    申请日：2011-04-27

    申请人： Sun-Chang Kim ,  Da-Jung Kim ,  Su-A Jang ,  Bong Hyun Sung ,  Ki-Jung Lim ,  Ju-Ri Shin ,  Young Woong Lee

    发明人： Sun-Chang Kim ,  Da-Jung Kim ,  Su-A Jang ,  Bong Hyun Sung ,  Ki-Jung Lim ,  Ju-Ri Shin ,  Young Woong Lee

    IPC分类号： A61K8/64 ,  A61K38/00 ,  A61K38/10

    CPC分类号： A61K38/10

    摘要： Disclosed are novel antimicrobial peptides which can promote the regeneration of skin cells, thus healing wounds. Pharmaceutical compositions comprising the peptides as active ingredients are also provided for wound healing and skin rejuvenation. The antimicrobial peptides exhibit inhibitory activity against antibiotic-resistant strains, and their antimicrobial activity is maintained without loss of structural stability even under a high salt condition. Also, being proven to promote the migration and regeneration of skin cells in mice as well as in vitro, the antimicrobial peptides may be widely used as an agent for regenerating skin cells. Further, they can find applications in various fields including the medical industry and the cosmetic industry. Hence, the novel antimicrobial peptides are anticipated to have considerable repercussions in the market for antibiotics, wound healing agents and cosmetics.

    摘要翻译： 公开了可以促进皮肤细胞再生的新型抗微生物肽，从而伤口愈合。 包含肽作为活性成分的药物组合物也用于伤口愈合和皮肤年轻化。 抗菌肽对抗生素抗性菌株表现出抑制活性，即使在高盐条件下，其抗微生物活性仍保持不失结构稳定性。 此外，被证明可促进小鼠和体外皮肤细胞的迁移和再生，抗微生物肽可以广泛用作再生皮肤细胞的药剂。 此外，他们可以在包括医疗行业和化妆品行业在内的各个领域找到应用。 因此，预期新型抗菌肽在市场上对抗生素，伤口愈合剂和化妆品具有相当大的影响。