公开(公告)号：US07700311B2

公开(公告)日：2010-04-20

申请号：US10593119

申请日：2005-03-10

申请人： Renate Schulze ,  Patrick Lorenz ,  Jürgen Eck ,  Oliver May ,  Harald Gröger ,  Harald Trauthwein

发明人： Renate Schulze ,  Patrick Lorenz ,  Jürgen Eck ,  Oliver May ,  Harald Gröger ,  Harald Trauthwein

IPC分类号： C12Q1/26 ,  C12N9/02

CPC分类号： C12P7/02 ,  C12N9/0006 ,  C12Y101/01002

摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to nonhuman hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohols which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohols with the formation of aldehydes or ketones.

摘要翻译： 本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。 本发明还涉及编码所述多肽的核酸，非人宿主细胞和宿主细胞以及可用于制备所需产物的反应体系。 本发明的多肽优选用于从醛或酮开始制备可用作药物中间体的伯和对映体纯的仲醇。 或者，本发明的多肽也可以用于逆反应，即醇形成醛或酮的氧化。

公开(公告)号：US20080216181A1

公开(公告)日：2008-09-04

申请号：US10593119

申请日：2005-03-10

申请人： Renate Schulze ,  Patrick Lorenz ,  Jurgen Eck ,  Oliver May ,  Harald Groger ,  Harald Trauthwein

发明人： Renate Schulze ,  Patrick Lorenz ,  Jurgen Eck ,  Oliver May ,  Harald Groger ,  Harald Trauthwein

IPC分类号： A01K67/027 ,  C12N9/02 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06

CPC分类号： C12P7/02 ,  C12N9/0006 ,  C12Y101/01002

摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to non-human hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohol's which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohol's with the formation of aldehydes or ketones.

摘要翻译： 本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。 本发明还涉及编码所述多肽，非人宿主或宿主细胞的核酸以及可用于制备所需产物的反应体系。 本发明的多肽优选用于从醛或酮开始制备可以作为药物中间体的初级和对映体纯的仲醇。 或者，也可以将本发明的多肽用于反向反应，即醇与醛或酮的形成的氧化。

公开(公告)号：US07749366B2

公开(公告)日：2010-07-06

申请号：US10525708

申请日：2003-08-20

申请人： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander H. Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jürgen Eck

发明人： Achim Quaiser ,  Torsten Ochsenreiter ,  Alexander H. Treusch ,  Arnulf Kletzin ,  Christa Schleper ,  Patrick Lorenz ,  Jürgen Eck

IPC分类号： G01N33/559 ,  C40B60/10 ,  C12M1/00

CPC分类号： C12N15/1003

摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

摘要翻译： 本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子 尺寸。 此外，本发明涉及用于分离核酸分子的方法，包括将样品施用于本发明的装置，其中所述核酸分子优选代表给定栖息地的元素的一部分。 此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。

公开(公告)号：US07300782B2

公开(公告)日：2007-11-27

申请号：US10872874

申请日：2004-06-21

申请人： Roland Breves ,  Karl-Heinz Maurer ,  Jürgen Eck ,  Patrick Lorenz ,  Holger Zinke

发明人： Roland Breves ,  Karl-Heinz Maurer ,  Jürgen Eck ,  Patrick Lorenz ,  Holger Zinke

IPC分类号： C12N9/24 ,  C12N9/00 ,  C11D3/386 ,  C07K1/00 ,  C07H21/04

CPC分类号： C12N9/2414 ,  C07K2319/00 ,  C11D3/386 ,  Y02E50/17

摘要： The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.

摘要翻译： 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸，基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品，以及与其对应的方法和可能的用途是特别有趣的。

公开(公告)号：US09045713B2

公开(公告)日：2015-06-02

申请号：US13502945

申请日：2010-10-27

申请人： Khanh Quoc Nguyen ,  Kornelia Titze ,  Tatiana Schwarz ,  Silvia Paladino ,  Volker Marschner ,  Patrick Lorenz

发明人： Khanh Quoc Nguyen ,  Kornelia Titze ,  Tatiana Schwarz ,  Silvia Paladino ,  Volker Marschner ,  Patrick Lorenz

IPC分类号： C12N9/00 ,  C12N9/20 ,  C12N1/20 ,  D06M16/00 ,  C12N15/00 ,  C11B3/00 ,  A23J7/00 ,  A23K1/165 ,  C12N9/16

CPC分类号： C12N9/18 ,  A23D9/00 ,  A23D9/02 ,  A23D9/04 ,  A23J7/00 ,  A23K20/189 ,  A23V2002/00 ,  C11B3/00 ,  C11B3/003 ,  C12N9/16 ,  C12Y301/01004 ,  C12Y301/01005 ,  C12Y301/01032

摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).

公开(公告)号：US20070212706A1

公开(公告)日：2007-09-13

申请号：US11552061

申请日：2006-10-23

申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

IPC分类号： C12Q1/68 ,  C12Q1/37 ,  C07H21/04 ,  C12P21/06 ,  C12N9/54 ,  C12N1/21 ,  C11D3/386 ,  C12N15/74

CPC分类号： C12N9/52 ,  C11D3/38609 ,  C11D3/38618

摘要： The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and C-terminally deleted, likewise proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

摘要翻译： 本申请涉及两个彼此相似的碱性蛋白酶（SEQ ID NO.4和7），其DNA是从土壤样品获得的，并且C末端缺失，同样蛋白水解活性片段（SEQ ID NO.5 和8），所有碱性蛋白酶类似于至少90％的SEQ ID NO。 4或至87.5％。 7，以及可以在源自SEQ ID NO：1的共有序列（SEQ ID NO.9）中总结的那些。 此外，它涉及与相关核酸（SEQ ID NO.3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。

公开(公告)号：US20050003419A1

公开(公告)日：2005-01-06

申请号：US10872874

申请日：2004-06-21

申请人： Roland Breves ,  Karl-Heinz Maurer ,  Jurgen Eck ,  Patrick Lorenz ,  Holger Zinke

发明人： Roland Breves ,  Karl-Heinz Maurer ,  Jurgen Eck ,  Patrick Lorenz ,  Holger Zinke

IPC分类号： D06L1/20 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/09 ,  C12N15/56 ,  C12P7/06 ,  C12P19/14 ,  C12Q1/68 ,  C12S11/00 ,  D06L4/40 ,  C07H21/04 ,  C12N9/20

CPC分类号： C12N9/2414 ,  C07K2319/00 ,  C11D3/386 ,  Y02E50/17

摘要： The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.

摘要翻译： 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸，基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品，以及与其对应的方法和可能的用途是特别有趣的。

公开(公告)号：US20070010417A1

公开(公告)日：2007-01-11

申请号：US11473708

申请日：2006-06-22

申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

IPC分类号： C11D3/386 ,  C07H21/04 ,  C12P21/06 ,  C12N9/54 ,  C12N1/21

CPC分类号： C11D3/386 ,  C12N9/50

摘要： The invention relates to a novel alkaline protease, the DNA thereof having been taken from a bottom sample, to all alkaline proteases that are at least 40% identical, and to all nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease, comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and to all alkaline proteases that are at least 60% identical, or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases and especially describes the use thereof in washing and cleaning products.

摘要翻译： 本发明涉及一种新的碱性蛋白酶，其DNA从底部样品中获取至所有与至少40％相同的碱性蛋白酶，以及所有与相关核酸具有至少50％同源性的核酸 。 本发明还涉及所述蛋白酶的片段，其除了编码该基因片段的氨基酸位置108至325之外，还涉及至少60％相同的所有碱性蛋白酶，或至少70个核酸 ％相同。 此外，本发明定义了用于所述蛋白酶的技术可能性，并且特别描述了在洗涤和清洁产品中的用途。

公开(公告)号：US20060112558A1

公开(公告)日：2006-06-01

申请号：US10999177

申请日：2004-11-29

申请人： Patrick Lorenz ,  Robert Johnson ,  Karl Klankowski ,  Curtis Hoehn ,  Robert Sugg

发明人： Patrick Lorenz ,  Robert Johnson ,  Karl Klankowski ,  Curtis Hoehn ,  Robert Sugg

IPC分类号： B21K1/16 ,  B21D51/16

CPC分类号： B21C1/24 ,  Y10T29/49435

摘要： A method for manufacturing a tube with varying wall thickness includes the step of drawing a tube over a generally cylindrical mandrel having an outer diameter that fluctuates along the length of the mandrel between a first diameter and a second diameter. The tube is drawn over the mandrel to form a tube wall having a thickness that fluctuates along the length of the tube between a first wall thickness and a second wall thickness. After the tube is drawn over the mandrel, the mandrel is removed from the tube. The outer surface of the tube may be finished to form a substantially uniform outer diameter along the length of the tube.

摘要翻译： 用于制造具有变化的壁厚的管的方法包括在大致圆柱形的心轴上拉制管的步骤，该心轴的外径沿心轴的长度在第一直径和第二直径之间波动。 管被拉过心轴以形成管壁，其具有在第一壁厚和第二壁厚之间沿着管的长度波动的厚度。 将管拉出心轴之后，从管中取出心轴。 管的外表面可以被精加工以沿着管的长度形成基本均匀的外径。

公开(公告)号：US20120213888A1

公开(公告)日：2012-08-23

申请号：US13502945

申请日：2010-10-27

申请人： Khanh Quoc Nguyen ,  Kornelia Titze ,  Tatiana Schwarz ,  Silvia Paladino ,  Volker Marschner ,  Patrick Lorenz

发明人： Khanh Quoc Nguyen ,  Kornelia Titze ,  Tatiana Schwarz ,  Silvia Paladino ,  Volker Marschner ,  Patrick Lorenz

IPC分类号： C12N9/16 ,  C12N15/63 ,  C12N1/15 ,  C12N1/19 ,  A23C9/12 ,  C11B3/02 ,  C12S11/00 ,  A23K1/165 ,  A21D8/04 ,  C12N15/55 ,  D06M16/00

CPC分类号： C12N9/18 ,  A23D9/00 ,  A23D9/02 ,  A23D9/04 ,  A23J7/00 ,  A23K20/189 ,  A23V2002/00 ,  C11B3/00 ,  C11B3/003 ,  C12N9/16 ,  C12Y301/01004 ,  C12Y301/01005 ,  C12Y301/01032

摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).