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14 条记录 (耗时 0.016 秒)

    Nucleic acid-containing polymerizable complex
    1.
    发明授权
    Nucleic acid-containing polymerizable complex 失效
    含有核酸的可聚合复合物

    公开(公告)号：US5932711A

    公开(公告)日：1999-08-03

    申请号：US812105

    申请日：1997-03-05

    申请人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    发明人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    IPC分类号： C07H21/04 ,  C07H21/00 ,  C07H21/02 ,  C08F20/34 ,  C08F20/60 ,  C07H19/00

    CPC分类号： C07H21/00 ,  C07H21/02

    摘要： Disclosed is a polymerizable complex containing a covalently attached nucleic acid molecule which, under appropriate conditions, is capable of copolymerization with a second polymerizable ethylene-containing monomer unit to form a polymerized layer. The polymerized layer containing attached nucleic acid is useful in a variety of contexts including, for example, hybridization assays. The polymerized layer containing the covalently attached nucleic acid molecule can be formed into a variety of shapes, or attached to a formed material through appropriate chemistry.

    摘要翻译： 公开了含有共价连接的核酸分子的可聚合复合物，其在合适的条件下能够与第二可聚合的含乙烯单体单元共聚以形成聚合层。 含有连接核酸的聚合层可用于多种情况，包括例如杂交测定。 含有共价连接的核酸分子的聚合层可以形成多种形状，或通过适当的化学成分连接到成形材料上。

    Denaturing gradient affinity electrophoresis and methods of use thereof
    2.
    发明授权
    Denaturing gradient affinity electrophoresis and methods of use thereof 失效
    变性梯度亲和电泳及其使用方法

    公开(公告)号：US06214187B1

    公开(公告)日：2001-04-10

    申请号：US09336228

    申请日：1999-06-18

    申请人： Philip W. Hammond ,  T. Christian Boles

    发明人： Philip W. Hammond ,  T. Christian Boles

    IPC分类号： B01D5702

    CPC分类号： C12Q1/6834 ,  C12Q1/68 ,  C12Q1/6827 ,  Y10T436/143333 ,  C12Q2565/519 ,  C12Q2565/125

    摘要： Methods are described for separation and analysis, and a kit for separation and analysis, of single nucleotide polymorphisms, or mutations, in target nucleic acid using denaturing gradient electrophoresis through supporting media containing one or more immobilized nucleic acid capture ligand. The method is especially useful for analyzing genetic haplotypes in samples with multiple linked polymorphic sites.

    摘要翻译： 描述用于分离和分析的方法，以及用于使用变性梯度电泳通过支持含有一种或多种固定化核酸捕获配体的培养基的靶核酸中单核苷酸多态性或突变进行分离和分析的试剂盒。 该方法对于分析具有多个连锁多态性位点的样品中的遗传单倍体特别有用。

    Nucleic acid-containing polymerizable complex
    5.
    发明授权
    Nucleic acid-containing polymerizable complex 有权
    含有核酸的可聚合复合物

    公开(公告)号：US06180770B2

    公开(公告)日：2001-01-30

    申请号：US09183092

    申请日：1998-10-30

    申请人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    发明人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C07H21/02

    摘要： Disclosed is a polymerizable complex containing a covalently attached nucleic acid molecule which, under appropriate conditions, is capable of copolymerization with a second polymerizable ethylene-containing monomer unit to form a polymerized layer. The polymerized layer containing attached nucleic acid is useful in a variety of contexts including, for example, hybridization assays. The polymerized layer containing the covalently attached nucleic acid molecule can be formed into a variety of shapes, or attached to a formed material through appropriate chemistry.

    摘要翻译： 公开了含有共价连接的核酸分子的可聚合复合物，其在合适的条件下能够与第二可聚合的含乙烯单体单元共聚以形成聚合层。 含有连接核酸的聚合层可用于多种情况，包括例如杂交测定。 含有共价连接的核酸分子的聚合层可以形成多种形状，或通过适当的化学成分连接到成形材料上。

    Systems and Methods for Processing Fluids
    7.
    发明申请
    Systems and Methods for Processing Fluids 有权
    处理流体的系统和方法

    公开(公告)号：US20130079251A1

    公开(公告)日：2013-03-28

    申请号：US13566806

    申请日：2012-08-03

    申请人： T. Christian Boles

    发明人： T. Christian Boles

    IPC分类号： C12N15/10 ,  C12M1/00 ,  C12P19/34

    CPC分类号： C12N15/1003 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2400/086 ,  C12M45/09 ,  C12N15/102 ,  C12P19/34 ,  C40B50/06

    摘要： Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing (“NGS”).

    摘要翻译： 公开了用于处理流体样品的系统和方法。 使用一系列微流体凸块阵列实现流体样品处理，其包括用于从生物样品分选颗粒的自动化和集成系统，裂解那些颗粒以暴露总RNA或DNA，纯化RNA或DNA，通过化学或处理RNA或DNA 酶修饰，按大小选择RNA或DNA分子，或产生任选的测序文库。 测序文库适用于下一代测序（NGS）。