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    EPITOPE MAPPING METHOD
    2.
    发明申请
    EPITOPE MAPPING METHOD 有权

    公开(公告)号：US20220084624A1

    公开(公告)日：2022-03-17

    申请号：US17536896

    申请日：2021-11-29

    申请人： The Scripps Research Institute

    发明人： Lars Hangartner ,  Andrew B. Ward ,  Matteo Bianchi ,  Hannah Turner

    IPC分类号： G16B15/00 ,  G16B25/20 ,  H01J37/26 ,  G01N33/564

    摘要： Provided herein are methods for mapping immune response to an immunogen, comprising: immunizing a subject with an immunogen and obtaining sera from the immunized subject at multiple time intervals following immunization, wherein the sera comprises one or more immune complexes between the immunogen and serum antibodies; imaging, by electron microscopy, the sera obtained from the immunized subject in each of the time intervals, to obtain structural images of the one or more immune complexes formed between the immunogen and serum antibodies; mapping immune response to the immunogen by measuring differences in structural images obtained at different time intervals to simultaneously visualize diverse antibodies targeting distinct epitopes in the immunized subjects. Further provided herein are vaccine design processes, comprising: administering a proposed vaccine to a test subject; imaging the immune complex formed in the test subject upon administration of the proposed vaccine; processing and visualizing the image to determine the likely immunogenicity of the proposed vaccine, and determining that the proposed vaccine is immunogenic if it binds to an antibody, and determining that the proposed vaccine should be redesigned if it does not bind or binds weakly to the antibody.