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公开(公告)号：US08715643B2
公开(公告)日：2014-05-06
申请号：US12936585
申请日：2009-03-06
CPC分类号： G01N33/5058 , C07K14/4711 , G01N33/5014
摘要： Disclosed is a transformed cell (a cell model) which can form a cytoplasmic inclusion body derived from TAR DNA-binding protein of 43 kDa (TDP-43) that is found in the brain of a patient suffering from a neurodegenerative disease such as FTLD and ALS. The transformed cell is characterized by having, introduced therein, a promoter capable of functioning in a host cell and a mutant TDP-43 gene.
摘要翻译： 公开了一种转化细胞（细胞模型），其可以形成来自患有神经变性疾病如FTLD的患者的脑中的43kDa（TDP-43）的TAR DNA结合蛋白的细胞质包涵体，以及 ALS。 转化细胞的特征在于在其中引入能够在宿主细胞中起作用的启动子和突变型TDP-43基因。
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公开(公告)号：US20110034447A1
公开(公告)日：2011-02-10
申请号：US12936585
申请日：2009-03-06
IPC分类号： A61K31/54 , C12N5/079 , C12Q1/68 , C07D279/18 , C07D471/04 , A61P25/28
CPC分类号： G01N33/5058 , C07K14/4711 , G01N33/5014
摘要： Disclosed is a transformed cell (a cell model) which can form a cytoplasmic inclusion body derived from TAR DNA-binding protein of 43 kDa (TDP-43) that is found in the brain of a patient suffering from a neurodegenerative disease such as FTLD and ALS. The transformed cell is characterized by having, introduced therein, a promoter capable of functioning in a host cell and a mutant TDP-43 gene.
摘要翻译： 公开了一种转化细胞（细胞模型），其可以形成来自患有神经变性疾病如FTLD的患者的脑中的43kDa（TDP-43）的TAR DNA结合蛋白的细胞质包涵体，以及 ALS。 转化细胞的特征在于在其中引入能够在宿主细胞中起作用的启动子和突变型TDP-43基因。
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公开(公告)号：US20110287453A1
公开(公告)日：2011-11-24
申请号：US12667624
申请日：2008-07-07
CPC分类号： C07K16/18 , G01N33/6875
摘要： The present invention provides an antibody that specifically binds to an abnormal TDP-43 protein aggregate, an agent comprising the antibody for detecting a TDP-43 proteinopathy lesion, and a method for detecting or diagnosing a TDP-43 proteinopathy lesion by using the antibody.
摘要翻译： 本发明提供特异性结合异常TDP-43蛋白质聚集体的抗体，包含用于检测TDP-43蛋白病变病变的抗体的试剂，以及使用该抗体检测或诊断TDP-43蛋白病变病变的方法。
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4.发明申请METHOD FOR PRODUCING INSOLUBLE AGGREGATE OF NEURODEGENERATIVE-DISEASE-RELATED PROTEIN 有权生产与神经营养因子相关蛋白质不溶性聚集体的方法公开(公告)号：US20140248657A1
公开(公告)日：2014-09-04
申请号：US14348024
申请日：2012-05-18
CPC分类号： C07K14/47 , C07K14/4711 , C12P21/02 , G01N33/5058 , G01N33/6896
摘要： The purpose of the present invention is to develop a method for producing a large amount of an insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient. A method of producing an insoluble aggregate of a neurodegenerative-disease-related protein according to the present invention comprises the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cultured cell in which the neurodegenerative-disease-related protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) extracting separating an insoluble fraction from the cultured cell. Optionally, the method may additionally comprise a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell.
摘要翻译： 本发明的目的是开发用于生产大量不溶性聚集体的方法，其相当于形成在患者脑中的不溶性聚集体。 根据本发明的生产神经变性疾病相关蛋白质的不溶性聚集体的方法包括以下步骤：（1）将来自神经变性疾病患者的脑的不溶性级分引入培养的细胞中，其中神经变性 - 疾病相关蛋白可以以组成型表达; （2）培养其中引入不溶性级分的培养细胞; 和（3）从培养的细胞中提取不溶性级分。 任选地，所述方法还可以包括扩增培养细胞中神经变性疾病相关蛋白质的不溶性聚集体的步骤。
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公开(公告)号：US08940872B2
公开(公告)日：2015-01-27
申请号：US12667624
申请日：2008-07-07
CPC分类号： C07K16/18 , G01N33/6875
摘要： The present invention provides an antibody that specifically binds to an abnormal TDP-43 protein aggregate, an agent comprising the antibody for detecting a TDP-43 proteinopathy lesion, and a method for detecting or diagnosing a TDP-43 proteinopathy lesion by using the antibody.
摘要翻译： 本发明提供特异性结合异常TDP-43蛋白质聚集体的抗体，包含用于检测TDP-43蛋白病变病变的抗体的试剂，以及使用该抗体检测或诊断TDP-43蛋白病变病变的方法。
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公开(公告)号：US09624279B2
公开(公告)日：2017-04-18
申请号：US14348024
申请日：2012-05-18
CPC分类号： C07K14/47 , C07K14/4711 , C12P21/02 , G01N33/5058 , G01N33/6896
摘要： The purpose of the present invention is to develop a method for amplifying in vitro to a large amount of a homogenous insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient. A method of producing an insoluble aggregate including TDP-43 protein and fragments thereof according to the present invention includes the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cell culture in which the intact TDP-43 protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) separating an insoluble fraction from the cultured cell. Optionally, the method may additionally include a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell.
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7.发明申请Cell Into Which Protein, Which Can Serve as Polymerization Nucleus of Protein Polymer, or Polymer Thereof Is Introduced, and Method for Production of The Cell 有权细胞进入可以作为蛋白质聚合物或其聚合物的聚合核的蛋白质，以及细胞的生产方法公开(公告)号：US20100047826A1
公开(公告)日：2010-02-25
申请号：US12086124
申请日：2006-12-06
CPC分类号： C07K14/47 , C12N5/0619 , C12N5/0622 , C12N2503/02 , C12N2510/00 , G01N33/5058 , G01N33/6896 , G01N2500/10
摘要： The present invention has the object of providing a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or polymer thereof is introduced, and a method for producing the cell. The invention relates to a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or a polymer thereof is introduced, a method for producing the cell, and a method of screening for a compound inhibiting an intracellular accumulation of a protein containing fibril structures, wherein the method comprises bringing a candidate substance into contact with the cell.
摘要翻译： 本发明的目的是提供一种细胞，其中可以引入蛋白质聚合物的聚合核的蛋白质或其聚合物，以及该细胞的制造方法。 本发明涉及引入可用作蛋白质聚合物的聚合核的蛋白质或其聚合物的细胞，细胞的制备方法和筛选抑制细胞内积累的化合物的方法 含有原纤维结构的蛋白质，其中所述方法包括使候选物质与细胞接触。
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公开(公告)号：US09802992B2
公开(公告)日：2017-10-31
申请号：US12086124
申请日：2006-12-06
IPC分类号： C12N5/079 , C12N5/0793 , G01N33/50 , C07K14/47 , G01N33/68
CPC分类号： C07K14/47 , C12N5/0619 , C12N5/0622 , C12N2503/02 , C12N2510/00 , G01N33/5058 , G01N33/6896 , G01N2500/10
摘要： The present invention has the object of providing a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or polymer thereof is introduced, and a method for producing the cell. The invention relates to a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or a polymer thereof is introduced, a method for producing the cell, and a method of screening for a compound inhibiting an intracellular accumulation of a protein containing fibril structures, wherein the method comprises bringing a candidate substance into contact with the cell.
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公开(公告)号：US20100254006A1
公开(公告)日：2010-10-07
申请号：US12740094
申请日：2008-10-31
申请人： Tomoyuki Ueno , Masato Hasegawa
发明人： Tomoyuki Ueno , Masato Hasegawa
CPC分类号： G02B5/1814 , B28B11/003 , B28B11/005 , C04B35/14 , C04B35/443 , C04B35/486 , C04B35/547 , C04B35/553 , C04B35/645 , C04B2235/446 , C04B2235/5436 , C04B2235/6581 , C04B2235/661 , C04B2235/94 , C04B2235/945 , C04B2235/963 , C04B2235/9653 , G02B5/1847
摘要： A diffractive optical element (1) composed of a ceramic, in which the optical characteristics are enhanced by enhancing the machining precision, is composed of an infrared-transmissive ceramic, and prominent portions (11) and groove portions (12) are repeatedly formed on a surface of the diffractive optical element (1). The average value of the surface roughness Ra within an optical effective area (10) of the surface of the diffractive optical element is 0.05 μm or less, and the difference in the surface roughness Ra within the optical effective area (10) of the surface is 0.02 μm or less.
摘要翻译： 通过提高加工精度提高光学特性的由陶瓷构成的衍射光学元件（1）由红外线透射性陶瓷构成，突出部（11）和槽部（12）重复形成在 衍射光学元件（1）的表面。 衍射光学元件的表面的光学有效面积（10）内的表面粗糙度Ra的平均值为0.05μm以下，表面光学有效面积（10）内的表面粗糙度Ra的差为 0.02μm以下。
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公开(公告)号：US20080199641A1
公开(公告)日：2008-08-21
申请号：US11905617
申请日：2007-10-02
申请人： Masato Hasegawa , Hiroki Okamura
发明人： Masato Hasegawa , Hiroki Okamura
CPC分类号： F16D13/64 , F16D69/02 , F16D2069/005 , F16D2069/0483 , F16D2069/0491 , F16D2250/00 , Y10T428/13 , Y10T428/21 , Y10T428/213 , Y10T428/218
摘要： In a wet-type friction material, a ring-shaped friction material substrate is compressed and fixed on a surface of a core metal by heat-pressing by a manufacturing metal mold. At the same time, a taper of an angle α corresponding to a taper at an outer peripheral side of an upper mold is provided on an outer peripheral side of the ring-shaped friction material substrate. Thus, the taper is formed such that a thickness in a radial direction of the ring-shaped friction material substrate becomes smaller from a middle point toward an outer peripheral edge.
摘要翻译： 在湿式摩擦材料中，通过制造金属模具的热压将环形摩擦材料基板压缩固定在芯金属的表面上。 同时，在环状摩擦材料基板的外周侧设置有与上模的外周侧的锥形相对应的角度α的锥度。 因此，锥形形成为使得环状摩擦材料基板的径向厚度从中点朝向外周边缘变小。
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