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    Cell culture device and manufacturing method thereof
    4.
    发明申请
    Cell culture device and manufacturing method thereof 审中-公开
    细胞培养装置及其制造方法

    公开(公告)号：US20060019378A1

    公开(公告)日：2006-01-26

    申请号：US11187621

    申请日：2005-07-21

    申请人： Takeshi Nagasaki ,  Seiji Shinkai ,  Atsushi Uno

    发明人： Takeshi Nagasaki ,  Seiji Shinkai ,  Atsushi Uno

    IPC分类号： C12M1/22

    CPC分类号： C12N5/0068 ,  C12N2533/12 ,  C12N2533/32

    摘要： A low-cost cell culture device with low cytotoxicity and improved cell adhesion is described, manufactured by a simplified coating process. At least the portion for cell culture on the surface of the cell culture device is coated with at least one of ε-poly-L-lysine, further polymerized derivatives of ε-poly-L-lysine and their salts.

    摘要翻译： 描述了通过简化的涂覆工艺制造的具有低细胞毒性和改善的细胞粘附性的低成本细胞培养装置。 至少在细胞培养装置表面上进行细胞培养的部分用ε-聚-L-赖氨酸，ε-聚-L-赖氨酸的其它聚合衍生物及其盐中的至少一种包被。

    Nuclear transport nucleic acid delivery vector
    5.
    发明授权
    Nuclear transport nucleic acid delivery vector 失效
    核转运核酸递送载体

    公开(公告)号：US07745596B2

    公开(公告)日：2010-06-29

    申请号：US11632138

    申请日：2005-07-11

    申请人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    发明人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    IPC分类号： C07H21/02 ,  C12N15/00

    CPC分类号： C12N15/88 ,  C07K2319/09 ,  C07K2319/23 ,  C12N15/87 ,  C12N2810/6072 ,  C12N2810/85

    摘要： To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.

    摘要翻译： 为了提供一种新的技术，通过该技术确保将靶基因递送到细胞中的有效转染，公开了一种用于核进入的核酸构建体，其包含由含有待递送至细胞核的基因的核酸物质组成的三元复合物 的细胞，能够通过核孔并参与核转运的importin蛋白（例如，进口蛋白）和结合物质（例如聚乙烯亚胺），其结合核酸物质和 importin蛋白。 通过施用与细胞膜受体结合因子和/或膜融合物质结合的核酸构建体，或者将包封在非病毒体内的核酸构建体施用，可以特别地促进从细胞外进入细胞核的核酸 具有细胞膜通透性和膜融合特性的载体（例如仙台病毒包膜）。

    Nucleic Acid Construct
    6.
    发明申请
    Nucleic Acid Construct 失效
    核酸构建体

    公开(公告)号：US20070281356A1

    公开(公告)日：2007-12-06

    申请号：US11632138

    申请日：2005-07-11

    申请人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    发明人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C12N15/88 ,  C07K2319/09 ,  C07K2319/23 ,  C12N15/87 ,  C12N2810/6072 ,  C12N2810/85

    摘要： To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.

    摘要翻译： 为了提供一种新的技术，通过该技术确保将靶基因递送到细胞中的有效转染，公开了一种用于核进入的核酸构建体，其包含由含有待递送至细胞核的基因的核酸物质组成的三元复合物 的细胞，能够穿过核孔并参与核转运的importin蛋白（例如，importin-β）以及结合核酸物质和进口蛋白的结合物质（例如聚乙烯亚胺） 蛋白。 通过施用与细胞膜受体结合因子和/或膜融合物质结合的核酸构建体，或者将包封在非病毒体内的核酸构建体施用，可以特别地促进从细胞外进入细胞核的核酸 具有细胞膜通透性和膜融合特性的载体（例如仙台病毒包膜）。

    Communication system, communication apparatus, and control method of relay apparatus
    7.
    发明授权
    Communication system, communication apparatus, and control method of relay apparatus 有权
    中继设备的通信系统，通信装置和控制方法

    公开(公告)号：US09167026B2

    公开(公告)日：2015-10-20

    申请号：US13291430

    申请日：2011-11-08

    申请人： Yusuke Shimada ,  Kazutaka Yamamoto ,  Hirokazu Banno ,  Takeshi Nagasaki ,  Masafumi Miyazawa ,  Satoshi Watanabe ,  Katsuaki Ito

    发明人： Yusuke Shimada ,  Kazutaka Yamamoto ,  Hirokazu Banno ,  Takeshi Nagasaki ,  Masafumi Miyazawa ,  Satoshi Watanabe ,  Katsuaki Ito

    IPC分类号： H04L29/08 ,  G06F17/30

    CPC分类号： H04L67/06 ,  G06F17/30274 ,  H04L67/2804 ,  H04L67/2819

    摘要： A communication system includes: a communication apparatus; and a relay apparatus. The communication apparatus includes: a contents-summary-information address request unit; a contents-summary-information display unit; a selection-information transmission unit; a selection-completion notification unit; and a contents get unit. The relay apparatus includes: an order-information addition unit that adds order information of contents to electronic data information; a contents-summary-information address transmission unit that transmits address information of a contents summary information item and the electronic data information, to which the order information is added, in response to a request of transmission of the address information of the contents summary information item; a contents-address get unit that gets the address information of the contents selected based on selection information from the selection-completion notification unit; and a contents-address transmission unit that transmits the address information of the contents got by the contents-address get unit to the communication apparatus.

    摘要翻译： 通信系统包括：通信装置; 和中继装置。 通信装置包括：内容摘要信息地址请求单元; 内容摘要信息显示单元; 选择信息发送单元; 选择完成通知单元; 和内容获取单元。 中继装置包括：向电子数据信息添加内容的订单信息的订单信息添加单元; 内容摘要信息地址发送单元，其响应于发送内容摘要信息项的地址信息的请求，发送内容摘要信息项目的地址信息和添加了订单信息的电子数据信息 ; 内容地址获取单元，其基于来自选择完成通知单元的选择信息获取所选择的内容的地址信息; 以及内容地址发送单元，其将由内容地址获取单元获取的内容的地址信息发送到通信装置。

    Controlling device
    8.
    发明授权
    Controlling device 有权
    控制装置

    公开(公告)号：US09143939B2

    公开(公告)日：2015-09-22

    申请号：US13966839

    申请日：2013-08-14

    申请人： Takeshi Nagasaki ,  Hideki Nogawa

    发明人： Takeshi Nagasaki ,  Hideki Nogawa

    IPC分类号： H04L29/06 ,  H04W12/06 ,  H04L12/24 ,  G06F11/00 ,  G06F12/14 ,  G06F12/16 ,  H04W88/02

    CPC分类号： H04W12/06 ,  H04L41/0806 ,  H04L41/0869 ,  H04W88/021

    摘要： A controlling device may acquire setting information regarding a wireless setting for a wireless communication currently being set in a wireless communication device. The controlling device may determine, using the setting information, whether the wireless setting indicates a first authentication method in which an authentication is performed by an authentication server or a second authentication method in which an authentication is performed by a device with which the wireless communication performing unit performs a wireless communication directly. The controlling device may provide a first screen to a displaying unit in a first case where a determination is made that the wireless setting indicates the first authentication method. The controlling device may provide a second screen which is different from the first screen to the displaying unit in a second case where a determination is made that the wireless setting indicates the second authentication method.

    摘要翻译： 控制装置可以获取关于无线通信装置中正在设定的无线通信的无线设定的设定信息。 控制装置可以使用设置信息来确定无线设置是否指示由认证服务器执行认证的第一认证方法或其中通过执行无线通信的设备执行认证的第二认证方法 单元直接执行无线通信。 在确定无线设置指示第一认证方法的第一种情况下，控制设备可以向显示单元提供第一屏幕。 在确定无线设置指示第二认证方法的第二种情况下，控制设备可以提供与第一屏幕不同的第二屏幕到显示单元。