INTRON-CONTAINING PROMOTERS AND USES THEREOF

    公开(公告)号:US20200377898A1

    公开(公告)日:2020-12-03

    申请号:US16070648

    申请日:2017-02-02

    IPC分类号: C12N15/81 C12P1/02

    摘要: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.

    METHODS FOR FERMENTATIVE PRODUCTION OF MASSOIA LACTONE

    公开(公告)号:US20230365915A1

    公开(公告)日:2023-11-16

    申请号:US18186257

    申请日:2023-03-20

    IPC分类号: C12N1/14 C12P17/06

    摘要: A culture for the fermentative production of Massoia lactone comprising an Aureobasidium melanogenum species in a culture medium, wherein the Aureobasidium melanogenum species expresses no functional Aureobasidin A synthase gene mRNA when cultured, the culture medium including KH2PO4, Na2HPO4, (NH4)2SO4, MgSO4·7H2O and CaCl2·2H2O; at least two trace elements selected from the group consisting of Fe2+, Cu2+, Zn2+ and MoO42−; urea; and a carbon source selected from the group consisting of glucose, mannose, xylose and mixtures thereof, wherein the culture medium has a pH from 5.5 to 6.5.