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公开(公告)号:US20190085345A1
公开(公告)日:2019-03-21
申请号:US16190386
申请日:2018-11-14
发明人: Lin CAI , Longhua SUN , Lin FU , Lianghui JI
IPC分类号: C12N15/82 , C12R1/01 , C12N1/20 , A01H4/00 , C07K14/195
CPC分类号: C12N15/8205 , A01H4/008 , C07K14/195 , C12N1/20 , C12R1/01
摘要: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
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公开(公告)号:US20190010444A1
公开(公告)日:2019-01-10
申请号:US15750584
申请日:2016-08-16
发明人: Lianghui JI , Si Te NGOH
摘要: The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.
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公开(公告)号:US20200157488A1
公开(公告)日:2020-05-21
申请号:US16712672
申请日:2019-12-12
发明人: Lianghui JI , Si Te NGOH
摘要: The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.
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公开(公告)号:US20200377898A1
公开(公告)日:2020-12-03
申请号:US16070648
申请日:2017-02-02
发明人: Lianghui JI , Yanbin LIU , Chong Mei John KOH , Sihui Amy YAP
摘要: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.
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5.
公开(公告)号:US20170362285A1
公开(公告)日:2017-12-21
申请号:US15535466
申请日:2015-12-14
发明人: Yanbin LIU , Chong Mei KOH , Lianghui JI
CPC分类号: C07K14/37 , C12N9/0008 , C12N15/113 , C12N15/80 , C12P21/02 , C12P23/00
摘要: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.
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公开(公告)号:US20230365915A1
公开(公告)日:2023-11-16
申请号:US18186257
申请日:2023-03-20
发明人: Lianghui JI , Si Te NGOH
CPC分类号: C12N1/14 , C12P17/06 , C12N1/145 , C12R2001/645
摘要: A culture for the fermentative production of Massoia lactone comprising an Aureobasidium melanogenum species in a culture medium, wherein the Aureobasidium melanogenum species expresses no functional Aureobasidin A synthase gene mRNA when cultured, the culture medium including KH2PO4, Na2HPO4, (NH4)2SO4, MgSO4·7H2O and CaCl2·2H2O; at least two trace elements selected from the group consisting of Fe2+, Cu2+, Zn2+ and MoO42−; urea; and a carbon source selected from the group consisting of glucose, mannose, xylose and mixtures thereof, wherein the culture medium has a pH from 5.5 to 6.5.
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公开(公告)号:US20180073034A1
公开(公告)日:2018-03-15
申请号:US15563176
申请日:2016-03-31
发明人: Lianghui JI , Yanbin LIU , Chong Mei John KOH
CPC分类号: C12N15/815 , C12N1/16 , C12N15/80 , C12N15/81 , C12N2830/36 , C12N2840/002
摘要: The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
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8.
公开(公告)号:US20170198315A1
公开(公告)日:2017-07-13
申请号:US15326077
申请日:2015-08-21
发明人: Yanbin LIU , Chong Mei KOH , Lianghui JI
CPC分类号: C12P7/6427 , C12N9/0006 , C12N9/0008 , C12N9/0071 , C12N9/1025 , C12N9/1029 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/81 , C12P7/6463 , C12Y101/0104 , C12Y102/01003 , C12Y102/01012 , C12Y114/19 , C12Y114/19001 , C12Y114/19003 , C12Y114/19006 , C12Y203/0102 , C12Y203/03008 , C12Y401/01001 , C12Y402/01011 , C12Y502/01008 , C12Y602/01003 , C12Y604/01002
摘要: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.
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