摘要:
The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).
摘要:
A beard trimming template is provided comprising a first elongated portion formed from a first straight edge having a first length, a and a second straight edge having a second length, the first and second straight edges positioned parallel to each other. A second elongated portion having a third straight edge and a fourth straight edge, the third and fourth straight edges positioned parallel to each other.A main body is formed by a radius bridging ends of the second and third straight edges proximal to the main body, and an apex, opposite the radius, formed by ends of the first and fourth straight edges proximal to the main body joining at a right angle. The first and second straight edges are connected via a fifth straight edge forming a first width edge between the first and second straight edges and the third and fourth straight edges are connected via a sixth straight edge forming a second width edge, and wherein the first and second width edges are equal in dimension and are distal to the main body.
摘要:
A method and apparatus for position decoding of three dimensional mesh models are described including predicting a symbol probability of a non-empty-child-cell Cl,k, where Cl,k denotes the kth cell at layer l, wherein the symbol probability is estimated based on an accuracy of a fitted plane P, decoding the non-empty-child-cell responsive to the received predicted probability of the non-empty-child-cell, subdividing the non-empty-child-cell, if the non-empty-child-cell has more than one vertex, determining if there are more unprocessed non-empty-child-cells at layer l, determining if a lowest layer of non-empty-child-cells has been reached, if there are no more unprocessed non-empty-child-cells at layer l and regenerating the three dimensional mesh model, if the lowest layer of non-empty-child-cells has been reached.
摘要:
An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.
摘要:
A beard trimming template is provided comprising a first elongated portion formed from a first straight edge and a second straight edge, the first and second edges being parallel. A second elongated portion has third and fourth straight edges, the third and fourth edges being parallel. A main body is formed by a radius bridging ends of the second and third straight edges proximal to the main body, and an apex, opposite the radius, formed by ends of the first and fourth straight edges proximal to the main body joining at a right angle. The first and second straight edges are connected via a fifth straight edge forming a first width edge, and the third and fourth straight edges are connected via a sixth straight edge forming a second width edge, wherein the first and second width edges are equal in dimension and distal to the main body.
摘要:
A method, device and system for implementing a long reach passive optical network (LR-PON) are provided, which solve the problem that the cost for establishing an LR-PON system is high. The method includes: receiving an uplink burst packet transmitted by an optical network unit (ONU) in a burst manner (101), converting a burst optical signal of the uplink burst packet into a continuous optical signal, and transmitting the continuous optical signal to a receiving device (105). The present invention is applicable to an LR-PON.
摘要:
Porous HCG scaffolds are provided in a perfusion bioreactor. Cells are seeded in the HCG scaffolds and cell culture media is perfused through the bioreactor to allow for cell seeding and growth. The cell culture media is removed, the HCG-cell constructs washed, and then preserved in the bioreactor with a perfusion comprising cryopreservation fluid comprising one or more of DMSO, trehalose, glycerol, ethylene glycol, or serum. The HCG-cell constructs (or the perfusion chambers containing them) are then removed from the bioreactor and placed in a cryopreservant media and maintained at about −80° C. The frozen HCG-cell constructs (or the chambers containing them) can then be stored at a suitable cryogenic temperature until needed. When needed, frozen HCG-cell constructs can be removed from cold storage and thawed using suitable means.
摘要:
Embodiments of the present invention disclose a method and a system for realizing PON reach extension, and a relay apparatus for realizing this method. The method includes: extracting an overhead in an upstream data stream that is sent by an ONU to an OLT, obtaining a payload in the upstream data stream through de-mapping, and storing it into a buffer; extracting bandwidth authorization information in an downstream data stream that is sent by the OLT to the ONU; and reorganizing a stored payload according to the bandwidth authorization information and inserting a corresponding overhead to generate a new upstream data stream and send it to the OLT.