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20 条记录 (耗时 0.031 秒)

    CONTROL OF METABOLIC FLUX IN CELL-FREE BIOSYNTHETIC SYSTEMS
    1.
    发明申请
    CONTROL OF METABOLIC FLUX IN CELL-FREE BIOSYNTHETIC SYSTEMS 审中-公开
    无细胞生物体系中代谢通量的控制

    公开(公告)号：US20160115558A1

    公开(公告)日：2016-04-28

    申请号：US14895992

    申请日：2014-06-05

    申请人： GREENLIGHT BIOSCIENCES, INC. ,  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

    发明人： James R. Swartz

    IPC分类号： C12Q3/00

    CPC分类号： C12Q3/00 ,  C12N9/00

    摘要： Methods are provided for controlling metabolic flux rate in a cell-free system comprising a complex set of enzymes, to produce a desired product of a pathway of interest. In the methods of the invention, measurements of metabolic performance parameters are taken by continuous monitoring or intermittent monitoring. Based on the metabolic performance parameters, the system is modified by one or more steps comprising: (i) altering enzyme levels in the cell-free system; (ii) altering feed rate of a substrate that controls redox flux or carbon flux to the cell-free system; (iii) altering O2 addition to the cell-free system; (iv) controlling efficiency of electron transport system by altering leakage across a membrane; wherein enzymes present in the pathway of interest catalyze production of a desired product.

    摘要翻译： 提供了用于控制包含复杂的酶组的无细胞系统中的代谢通过速率的方法，以产生感兴趣的途径的所需产物。 在本发明的方法中，通过连续监测或间歇监测进行代谢性能参数的测量。 基于代谢性能参数，系统通过一个或多个步骤修饰，包括：（i）改变无细胞系统中的酶水平; （ii）将控制氧化还原通量或碳通量的底物的进料速率改变为无细胞系统; （iii）改变无细胞系统中的O2添加; （iv）通过改变膜上的渗漏来控制电子传输系统的效率; 其中存在于感兴趣途径中的酶催化产生所需产物。

    METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS THROUGH PROTEASE MANIPULATION
    2.
    发明申请
    METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS THROUGH PROTEASE MANIPULATION 审中-公开
    通过蛋白质调控控制代谢途径中的通量的方法

    公开(公告)号：US20150064751A1

    公开(公告)日：2015-03-05

    申请号：US14542074

    申请日：2014-11-14

    申请人： The Board of Trustees of the Leland Stanford Junior University ,  GreenLight Biosciences, Inc.

    发明人： James R. Swartz

    IPC分类号： C12N9/50 ,  C12P7/42 ,  C12N15/70

    CPC分类号： C12N9/50 ,  C12N15/52 ,  C12N15/70 ,  C12P7/42 ,  C12P19/305 ,  C12Y304/21009

    摘要： The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.

    摘要翻译： 本文描述的实施方案涉及细胞和用于制备细胞的方法，其可以通过改变竞争基底或感兴趣途径的产物的酶使其用作生物催化剂，使得靶向酶对位点特异性蛋白酶敏感，其中 蛋白酶被表达，但是在细胞中重新定位到与完整细胞中的目标酶不接触的位点。 细胞裂解后，蛋白酶与靶酶接触，然后通过蛋白酶切割失活。

    Hydrogenase Fusion Protein for Improved Hydrogen Production
    6.
    发明申请
    Hydrogenase Fusion Protein for Improved Hydrogen Production 有权
    氢化酶融合蛋白，用于改善氢生产

    公开(公告)号：US20130273628A1

    公开(公告)日：2013-10-17

    申请号：US13791550

    申请日：2013-03-08

    申请人： The Board of Trustees of the Leland Stanford Junior University

    发明人： Phillip Richard Smith ,  James R. Swartz

    IPC分类号： C12P3/00 ,  C12N9/96

    CPC分类号： C12P3/00 ,  C07K2319/00 ,  C12N9/0067 ,  C12N9/0095 ,  C12N9/96 ,  C12Y112/07002 ,  C12Y118/01002

    摘要： Compositions of a fusion protein comprising a spatially tethered ferredoxin-NADP-reductase (FNR) and an active [FeFe] hydrogenase, genetic sequences encoding such fusion proteins, and methods of use thereof are provided. The fusion proteins of the invention link an FNR polypeptide to an active [FeFe] hydrogenase through a polypeptide linker. The fusion protein facilitates improved electron transfer through a ferredoxin, and allows direct electron transfer from NADPH to the hydrogenase.

    摘要翻译： 提供了包含空间连接的铁氧还蛋白-NADP还原酶（FNR）和活性[FeFe]氢化酶，编码这种融合蛋白的遗传序列及其使用方法的融合蛋白的组合物。 本发明的融合蛋白通过多肽接头将FNR多肽与活性[FeFe]氢化酶连接。 融合蛋白促进了通过铁氧还蛋白的电子转移，并允许从NADPH直接电子转移到氢化酶。

    SYNTHESIS OF LINEAR AND BRANCHED POLYMERS OF POLYPEPTIDES THROUGH DIRECT CONJUGATION
    7.
    发明申请
    SYNTHESIS OF LINEAR AND BRANCHED POLYMERS OF POLYPEPTIDES THROUGH DIRECT CONJUGATION 有权
    通过直接结合合成多肽的线性和分支聚合物

    公开(公告)号：US20140315276A1

    公开(公告)日：2014-10-23

    申请号：US14196851

    申请日：2014-03-04

    申请人： The Board of Trustees of the Leland Stanford Junior University

    发明人： Cem Albayrak ,  James R. Swartz ,  Yuan Lu

    IPC分类号： C12N7/00 ,  C07K14/005 ,  C12P21/00

    CPC分类号： C12N7/00 ,  C07K1/1075 ,  C07K14/005 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10151 ,  C12P21/02

    摘要： Methods are provided for a one step synthesis of polypeptide polymers or co-polymers. The polymers or co-polymers can be linear or branched. In the methods of the invention, the coding sequence for the polypeptide(s) to be polymerized is altered by introducing one or more codons for an nonnatural amino acid, which coding sequence is then utilized to produce the cognate polypeptide. The nonnatural amino acids are selected to be reactive with each other in a bioorthogonal reaction, and are combined in a conjugation reaction with the desired components of the polymer.

    摘要翻译： 提供了一步合成多肽聚合物或共聚物的方法。 聚合物或共聚物可以是直链或支链的。 在本发明的方法中，通过为非天然氨基酸引入一个或多个密码子来改变要聚合的多肽的编码序列，然后将该编码序列用于产生同源多肽。 选择非天然氨基酸在生物正交反应中彼此反应，并将其与共聚反应与聚合物的所需组分结合。