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公开(公告)号：US11890620B2
公开(公告)日：2024-02-06
申请号：US16755505
申请日：2018-10-12
IPC分类号： B01L3/00 , B01L7/00 , B04B3/00 , B04B5/04 , C12Q1/6837 , C12Q1/6874
CPC分类号： B01L3/50853 , B01L3/502715 , B01L3/502746 , B01L7/00 , B04B3/00 , B04B5/0414 , C12Q1/6837 , C12Q1/6874 , B01L2200/0689 , B01L2200/16 , B01L2300/044 , B01L2300/0636 , B01L2300/168
摘要： Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.
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公开(公告)号：US11591561B2
公开(公告)日：2023-02-28
申请号：US16168464
申请日：2018-10-23
发明人： Vishal Tandon , Charles A. Lissandrello , Ryan A. Dubay , Rebecca Christianson , Jenna Leigh Balestrini , Peter Hsi , Jason Fiering
摘要： Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.
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公开(公告)号：US11859162B2
公开(公告)日：2024-01-02
申请号：US16557820
申请日：2019-08-30
发明人： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
CPC分类号： C12M23/16 , C12M35/02 , B01L3/50273 , B01L3/502715 , B01L2300/047 , B01L2300/087 , B01L2300/0864 , B01L2300/0887 , B01L2400/0496 , C12N15/87
摘要： Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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公开(公告)号：US11385400B2
公开(公告)日：2022-07-12
申请号：US15811203
申请日：2017-11-13
发明人： Jesse J. Wheeler , Joseph J. Register , Parshant Kumar , Carlos A. Segura , Charles A. Lissandrello , John J. LeBlanc
IPC分类号： G02B6/02 , A61B5/00 , G02B6/12 , A61N5/06 , B05D1/00 , B05D3/10 , B05D3/12 , G02B1/04 , G02B6/26
摘要： The material stack of the present disclosure can be used for fabricating optical waveguides that are thin and flexible, and that can bend light around small turns. The stack of materials can include a polymer core and a cladding, which together can create a large difference in refractive index. As a result, light can remain within the core even when bent around radii where standard glass fibers could fail.
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公开(公告)号：US20240084236A1
公开(公告)日：2024-03-14
申请号：US18515493
申请日：2023-11-21
发明人： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
CPC分类号： C12M23/16 , C12M35/02 , B01L3/502715
摘要： Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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公开(公告)号：US20200071727A1
公开(公告)日：2020-03-05
申请号：US16557820
申请日：2019-08-30
发明人： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
摘要： Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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公开(公告)号：US20210220823A1
公开(公告)日：2021-07-22
申请号：US16755505
申请日：2018-10-12
IPC分类号： B01L3/00 , C12Q1/6874 , B01L7/00 , C12Q1/6837 , B04B5/04 , B04B3/00
摘要： Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.
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公开(公告)号：US20190292565A1
公开(公告)日：2019-09-26
申请号：US16359626
申请日：2019-03-20
摘要： A system for sequential exposure of particles to different fluid streams includes an acoustic actuator device for acoustically driving one or more substrates and a microchannel device of the one or more substrates that receive particles in a first flowing fluid, moves the particles to a second flowing fluid, then moves the particles out of the second flowing fluid using acoustic radiation generated by the acoustic actuator device. The system can control residence times in the streams. According to one use, the first flowing fluid is a cell buffer and the second flowing media is an electroporation buffer. An electroporation system is placed in or downstream of the acoustic actuator device. However, in other uses, the second flowing media might be a wash buffer.
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公开(公告)号：US20180136389A1
公开(公告)日：2018-05-17
申请号：US15811203
申请日：2017-11-13
发明人： Jesse J. Wheeler , Joseph J. Register , Parshant Kumar , Carlos A. Segura , Charles A. Lissandrello , John J. LeBlanc
CPC分类号： G02B6/02033 , A61B5/6846 , A61N5/0601 , A61N2005/0612 , B05D1/005 , B05D3/107 , B05D3/12 , G02B1/046 , G02B1/048 , G02B6/12 , G02B6/262 , G02B2006/12069 , G02B2006/12138
摘要： The material stack of the present disclosure can be used for fabricating optical waveguides that are thin and flexible, and that can bend light around small turns. The stack of materials can include a polymer core and a cladding, which together can create a large difference in refractive index. As a result, light can remain within the core even when bent around radii where standard glass fibers could fail.
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公开(公告)号：US20230183631A1
公开(公告)日：2023-06-15
申请号：US18067901
申请日：2022-12-19
发明人： Vishal Tandon , Charles A. Lissandrello , Ryan A. Dubay , Rebecca Christianson , Jenna Leigh Balestrini , Peter Hsi , Jason Fiering
CPC分类号： C12M35/02 , A61K9/0009 , C12M35/04 , C12N15/87 , C12M23/16 , B01L3/502715 , B01L3/502761 , A61N1/327 , B01L3/50273 , B82Y5/00
摘要： Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.
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