公开(公告)号：US20170175085A1

公开(公告)日：2017-06-22

申请号：US15440424

申请日：2017-02-23

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servi

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12N5/074 ,  G01N33/574 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20180119113A1

公开(公告)日：2018-05-03

申请号：US15440815

申请日：2017-02-23

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servi

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12N9/02 ,  G01N33/53 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873

CPC分类号： C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00 ,  C12Q2537/164 ,  C12Q2522/10 ,  C12Q2521/531

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20240254542A1

公开(公告)日：2024-08-01

申请号：US18588987

申请日：2024-02-27

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TEM3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20180224434A9

公开(公告)日：2018-08-09

申请号：US15440826

申请日：2017-02-23

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servic

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： G01N33/53 ,  G01N33/574 ,  C12N9/02

CPC分类号： C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00 ,  C12Q2537/164 ,  C12Q2522/10 ,  C12Q2521/531

摘要： Provided herein are methods and kits for measuring a level of a 5-hydroxymethylcytosine in a nucleotide sequence from a subject, wherein the subject is a subject having a cancer or suspected of having cancer.

公开(公告)号：US20180044633A1

公开(公告)日：2018-02-15

申请号：US15722202

申请日：2017-10-02

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servic

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12N5/0783

CPC分类号： C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20170176420A1

公开(公告)日：2017-06-22

申请号：US15440319

申请日：2017-02-23

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servic

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： G01N33/53 ,  C12N5/074 ,  G01N33/574

CPC分类号： C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20240318235A1

公开(公告)日：2024-09-26

申请号：US18739955

申请日：2024-06-11

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC分类号： C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20210246494A1

公开(公告)日：2021-08-12

申请号：US17231671

申请日：2021-04-15

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/26 ,  G01N33/53 ,  C12Q1/6806 ,  C12Q1/6869

摘要： The present invention provides for methods of epigenetic analysis. In some cases, the methods may include obtaining a sample comprising a nucleic acid sequence. In some cases, the nucleic acid sequence may comprise one or more epigenetic marks. The methods may include performing a sequencing. The methods may include distinguishing a hydroxymethylated base from a methylated base.

公开(公告)号：US20200040381A1

公开(公告)日：2020-02-06

申请号：US16658195

申请日：2019-10-21

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/6827 ,  C12Q1/26 ,  G01N33/50 ,  C12N9/02 ,  G01N33/53 ,  C12N5/00 ,  C12Q1/6806 ,  C12N5/074 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873 ,  C12Q1/6869 ,  C12Q1/6886

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US10465234B2

公开(公告)日：2019-11-05

申请号：US16169801

申请日：2018-10-24

申请人： The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

发明人： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC分类号： C12Q1/26 ,  C12Q1/6827 ,  G01N33/50 ,  C12N9/02 ,  G01N33/53 ,  C12N5/00 ,  C12Q1/6806 ,  C12N5/074 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873 ,  C12Q1/6869 ,  C12Q1/6886

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.