利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

44 条记录 (耗时 0.059 秒)

    Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
    7.
    发明申请
    Engineered microorganisms for increasing product yield in biotransformations, related methods and systems 审中-公开
    用于提高生物转化，相关方法和系统中产品产量的工程微生物

    公开(公告)号：US20080293101A1

    公开(公告)日：2008-11-27

    申请号：US11881748

    申请日：2007-07-27

    申请人： Matthew W. Peters ,  Peter Meinhold ,  Thomas Buelter ,  Marco Landwehr

    发明人： Matthew W. Peters ,  Peter Meinhold ,  Thomas Buelter ,  Marco Landwehr

    IPC分类号： C12P21/04 ,  C12N1/20 ,  C12P7/04 ,  C12M1/00

    CPC分类号： C12N9/0036

    摘要： There are disclosed recombinant microorganisms engineered to increase product yield in a biotransformation. In an embodiment, the microorganisms are engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. There are also disclosed methods and systems for using recombinant microorganisms engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. Other embodiments are also disclosed.

    摘要翻译： 公开了在生物转化中改进产物产率的重组微生物。 在一个实施方案中，将微生物工程化以增加可用于参与生物转化的NAD（P）H需氧化还原酶的NAD（P）H的量。 还公开了使用重组微生物进行工程改造以增加参与生物转化的NAD（P）H需氧化还原酶可用的NAD（P）H量的方法和系统。 还公开了其他实施例。

    ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS
    8.
    发明申请
    ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS 审中-公开
    用于生产N-丁醇的工程微生物及相关方法

    公开(公告)号：US20090155869A1

    公开(公告)日：2009-06-18

    申请号：US11949724

    申请日：2007-12-03

    申请人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

    发明人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

    IPC分类号： C12P7/16 ,  C12N1/21

    CPC分类号： C12N15/52

    摘要： A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption.

    摘要翻译： 至少表达NADH依赖性途径的异源酶的重组微生物，用于将碳源转化为正丁醇，代谢中间体和/或其衍生物，并能够生产正丁醇，代谢中间体和/或衍生物 以高产率和相关方法。 重组微生物被工程化以使与NADH依赖性异源途径竞争的一种或多种途径的天然酶失活，和/或平衡NADH依赖性异源途径相对于NADH生产和消耗。

    ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS
    9.
    发明申请
    ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
    在无机条件下生产目标化合物的工程化微生物

    公开(公告)号：US20120190089A1

    公开(公告)日：2012-07-26

    申请号：US13303884

    申请日：2011-11-23

    申请人： Thomas Buelter ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Andrew C. Hawkins ,  Sabine Bastian ,  Frances Arnold ,  Jun Urano

    发明人： Thomas Buelter ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Andrew C. Hawkins ,  Sabine Bastian ,  Frances Arnold ,  Jun Urano

    IPC分类号： C12P7/16 ,  C12N1/19

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0036 ,  Y02E50/10

    摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

    摘要翻译： 本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。 本发明还提供已被突变或修饰以提高其NADH依赖性活性或​​将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。 此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。 还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。