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    Methods for inducing differentiation of embryonic stem cells and uses thereof
    1.
    发明授权
    Methods for inducing differentiation of embryonic stem cells and uses thereof 有权
    诱导胚胎干细胞分化的方法及其用途

    公开(公告)号：US07390659B2

    公开(公告)日：2008-06-24

    申请号：US10196882

    申请日：2002-07-16

    申请人： Thomas Jessell ,  Hynek Wichterle ,  Ivo Lieberam

    发明人： Thomas Jessell ,  Hynek Wichterle ,  Ivo Lieberam

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0619 ,  A61K35/12 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2506/02 ,  Y02A50/465

    摘要： The present invention provides a method for inducing differentiation of an embryonic stem cell into a differentiated neural cell. The present invention further provides a method for producing differentiated neural cells, and a population of cells comprising the differentiated neural cells. Additionally, the present invention provides a method for repopulating a spinal cord in a subject, and a method for treating nervous tissue degeneration in a subject in need of treatment. The present invention further provides neural progenitor cells, differentiated neural cells, and uses of same. Also provided is a transgenic non-human animal containing the differentiated neural cells. The present invention is further directed to a method for isolating a population of differentiated neural cells. Finally, the present invention provides a method for identifying an agent for use in treating a condition associated with neuron degeneration.

    摘要翻译： 本发明提供了诱导胚胎干细胞分化为分化神经细胞的方法。 本发明还提供了用于产生分化的神经细胞的方法和包含分化的神经细胞的细胞群。 另外，本发明提供了一种用于在受试者中重新填充脊髓的方法，以及用于治疗需要治疗的受试者中的神经组织变性的方法。 本发明还提供神经祖细胞，分化神经细胞及其用途。 还提供了含有分化的神经细胞的转基因非人动物。 本发明还涉及分离分化的神经细胞群体的方法。 最后，本发明提供一种用于鉴定用于治疗与神经元变性相关的病症的药剂的方法。

    STEM CELL-BASED CULTURE SYSTEM FOR DRUG DEVELOPMENT
    5.
    发明申请
    STEM CELL-BASED CULTURE SYSTEM FOR DRUG DEVELOPMENT 有权
    用于药物开发的基于干细胞的文化系统

    公开(公告)号：US20100267073A1

    公开(公告)日：2010-10-21

    申请号：US12450830

    申请日：2008-04-10

    申请人： Serge Przedborski ,  Hynek Wichterle ,  Makiko Nagai ,  Thomas M. Jessell

    发明人： Serge Przedborski ,  Hynek Wichterle ,  Makiko Nagai ,  Thomas M. Jessell

    IPC分类号： C12Q1/02 ,  C12N5/079 ,  C12N5/0793

    CPC分类号： C12N5/0619 ,  C12N2502/08 ,  C12N2506/02

    摘要： The present invention relates to culture systems comprising differentiated stem cells, that may be used for identifying agents useful in treating degenerative nervous system disorders and are suitable for high-throughput screening applications. It is based, at least in part, on the discovery that co-cultures of (i) astrocytes expressing a mutated SODI gene and (ii) stem-cell derived motor neurons manifested cell death via a Bax-dependent mechanism, and modeled motor neuron death in amyotrophic lateral sclerosis.

    摘要翻译： 本发明涉及包含分化干细胞的培养系统，其可用于鉴定可用于治疗退行性神经系统疾病并且适用于高通量筛选应用的药剂。 至少部分基于以下发现：（i）表达突变的SODI基因的星形胶质细胞和（ii）干细胞来源的运动神经元的共培养物通过Bax依赖性机制表现出细胞死亡，并建模运动神经元 肌萎缩性侧索硬化症死亡。

    Generation of brachial, thoracic and lumbar spinal motor neurons from embryonic stem cells in the absence of all-trans retinoic acid supplement
    6.
    发明申请
    Generation of brachial, thoracic and lumbar spinal motor neurons from embryonic stem cells in the absence of all-trans retinoic acid supplement 有权
    在不存在全反式维甲酸补充剂的情况下从胚胎干细胞中产生臂，胸和腰椎运动神经元

    公开(公告)号：US20100196332A1

    公开(公告)日：2010-08-05

    申请号：US12653217

    申请日：2009-12-09

    申请人： Hynek Wichterle ,  Thomas M. Jessell ,  Mirza Peljto

    发明人： Hynek Wichterle ,  Thomas M. Jessell ,  Mirza Peljto

    IPC分类号： A61K35/12 ,  C12N5/02 ,  C12N5/071 ,  C12N5/07 ,  A61K38/00

    CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2506/02 ,  C12N2506/45

    摘要： Disclosed are methods for generating a neuron expressing Hoxc8 transcription factor or a caudal motor neuron comprising culturing an embryonic stem cell in a composition which is essentially free of retinoids and comprises an isotonic salt solution, so as to generate the neuron which expresses Hoxc8 transcription factor or the caudal motor neuron. Disclosed are also methods for generating a caudal brachial motor neuron, a thoracic motor neuron, or a lumbar motor neuron from an embryonic stem cell in a composition essentially free of retinoids and comprising ADFNK medium, an amount of FGF-2, or Gdf11 respectively. Disclosed are also methods of transplanting a motor neuron into a subject comprising generating the motor neuron and transplanting the motor neuron into the subject. Disclosed is also a population of motor neuron cells enriched for motor neuron cells expressing Foxp1 and expressing a gene associated with Spinal Muscular Atrophy (SMA) or Amyotrophic Lateral Sclerosis (ALS).

    摘要翻译： 公开了产生表达Hoxc8转录因子或尾部运动神经元的神经元的方法，包括在基本上不含类视黄醇的组合物中培养胚胎干细胞，并且包含等渗盐溶液，以产生表达Hoxc8转录因子的神经元或 尾部运动神经元。 还公开了在基本上不含类视黄醇的组合物中产生来自胚胎干细胞的尾部肱动脉神经元，胸部运动神经元或腰运动神经元的方法，并且分别包含一定量的FGF-2或Gdf11的ADFNK培养基。 还公开了将运动神经元移植到受试者中的方法，包括产生运动神经元并将运动神经元移植到受试者中。 还公开了富含表达Foxp1并表达与脊髓性肌萎缩（SMA）或肌萎缩性侧索硬化（ALS）相关的基因的运动神经元细胞的运动神经元细胞的群体。

    Caudal motor neuron derived from embryonic stem cells under conditions essentially free of any retinoid
    10.
    发明授权
    Caudal motor neuron derived from embryonic stem cells under conditions essentially free of any retinoid 有权
    尾状运动神经元衍生自胚胎干细胞条件基本上没有任何类视黄醇

    公开(公告)号：US08969081B2

    公开(公告)日：2015-03-03

    申请号：US12653217

    申请日：2009-12-09

    申请人： Hynek Wichterle ,  Thomas M. Jessell ,  Mirza Peljto

    发明人： Hynek Wichterle ,  Thomas M. Jessell ,  Mirza Peljto

    IPC分类号： C12N5/02 ,  C12N5/0793 ,  C12N5/074 ,  C12N5/0735 ,  C12N5/0797 ,  A61K35/30

    CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2506/02 ,  C12N2506/45

    摘要： Disclosed are methods for generating a neuron expressing Hoxc8 transcription factor or a caudal motor neuron comprising culturing an embryonic stem cell in a composition which is essentially free of retinoids and comprises an isotonic salt solution, so as to generate the neuron which expresses Hoxc8 transcription factor or the caudal motor neuron. Disclosed are also methods for generating a caudal brachial motor neuron, a thoracic motor neuron, or a lumbar motor neuron from an embryonic stem cell in a composition essentially free of retinoids and comprising ADFNK medium, an amount of FGF-2, or Gdf11 respectively. Disclosed are also methods of transplanting a motor neuron into a subject comprising generating the motor neuron and transplanting the motor neuron into the subject. Disclosed is also a population of motor neuron cells enriched for motor neuron cells expressing Foxp1 and expressing a gene associated with Spinal Muscular Atrophy (SMA) or Amyotrophic Lateral Sclerosis (ALS).

    摘要翻译： 公开了产生表达Hoxc8转录因子或尾部运动神经元的神经元的方法，包括在基本上不含类视黄醇的组合物中培养胚胎干细胞，并且包含等渗盐溶液，以产生表达Hoxc8转录因子的神经元或 尾部运动神经元。 还公开了在基本上不含类视黄醇的组合物中产生来自胚胎干细胞的尾部肱动脉神经元，胸部运动神经元或腰运动神经元的方法，并且分别包含一定量的FGF-2或Gdf11的ADFNK培养基。 还公开了将运动神经元移植到受试者中的方法，包括产生运动神经元并将运动神经元移植到受试者中。 还公开了富含表达Foxp1并表达与脊髓性肌萎缩（SMA）或肌萎缩性侧索硬化（ALS）相关的基因的运动神经元细胞的运动神经元细胞的群体。