公开(公告)号：US06458560B1

公开(公告)日：2002-10-01

申请号：US09415868

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilva Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilva Frolov

IPC分类号： C12P2106

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US06451592B1

公开(公告)日：2002-09-17

申请号：US08944465

申请日：1997-10-06

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C12N1500

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

公开(公告)号：US06465634B1

公开(公告)日：2002-10-15

申请号：US09415900

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C07H2104

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

公开(公告)号：US06391632B1

公开(公告)日：2002-05-21

申请号：US09415784

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Dryga ,  Ilya Frolov

IPC分类号： C12N510

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as is compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质，与野生型重组甲病毒颗粒相比。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US07811812B2

公开(公告)日：2010-10-12

申请号：US10391441

申请日：2003-03-17

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Drvga ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Barbara A. Belli ,  Sondra Schlesinger ,  Sergey A. Drvga ,  Ilya Frolov

IPC分类号： C12N15/63

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子，其包含甲病毒非结构蛋白基因，当可操作地并入重组甲病毒颗粒中时，与野生型相比，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平 类型，以及与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的相同或更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US06426196B1

公开(公告)日：2002-07-30

申请号：US09415785

申请日：1999-10-08

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Sondra Schlesinger ,  Ilya Frolov

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Sondra Schlesinger ,  Ilya Frolov

IPC分类号： C12P2106

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/79 ,  C12N15/86 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： Isolated nucleic acid molecules are disclosed. comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.

摘要翻译： 公开了分离的核酸分子。 包括甲型病毒非结构蛋白基因，当与野生型相比，当可操作地并入重组甲病毒颗粒中时，真核分子载体启动系统或RNA载体复制子具有降低的载体特异性RNA合成水平，并且相同或 与野生型重组甲病毒颗粒相比，从病毒连接区启动子转录的RNA编码的更高水平的蛋白质。 还公开了RNA载体复制子，甲病毒载体构建体和含有上述核酸分子的真核分子载体启动系统。

公开(公告)号：US5843723A

公开(公告)日：1998-12-01

申请号：US739167

申请日：1996-10-30

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12N5/10

CPC分类号： C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present invention provides compositions and method,, for utilizing recombinant alphavirus vectors.

摘要翻译： 本发明提供了利用重组甲病毒载体的组合物和方法。

公开(公告)号：US07977091B2

公开(公告)日：2011-07-12

申请号：US12538494

申请日：2009-08-10

申请人： Thomas W. Dubensky, Jr. ,  John M Polo ,  Carlos E Ibanez ,  Stephen M. W. Chang ,  Douglas J Jolly ,  David A Driver ,  Barbara A Belli

发明人： Thomas W. Dubensky, Jr. ,  John M Polo ,  Carlos E Ibanez ,  Stephen M. W. Chang ,  Douglas J Jolly ,  David A Driver ,  Barbara A Belli

IPC分类号： C12N15/63 ,  C12Q1/68

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present disclosure provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

摘要翻译： 本公开提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US07572628B2

公开(公告)日：2009-08-11

申请号：US10346880

申请日：2003-01-16

申请人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

发明人： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC分类号： C12N15/63 ,  C12N15/85 ,  A61K48/00 ,  C07H21/04

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

摘要： The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

摘要翻译： 本发明提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US06767699B2

公开(公告)日：2004-07-27

申请号：US09872086

申请日：2001-05-31

申请人： John M. Polo ,  Catherine Greer ,  Thomas W. Dubensky, Jr.

发明人： John M. Polo ,  Catherine Greer ,  Thomas W. Dubensky, Jr.

IPC分类号： C12Q170

CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2770/36151 ,  C12Q1/6897 ,  C12Q1/701

摘要： Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics.

摘要翻译： 公开了病毒和病毒衍生载体的生产和纯化方法，包括与甲病毒相关的方法。 一方面，提供将甲病毒复制子颗粒制备物进行一步或多步色谱纯化的纯化方法，例如使用离子交换树脂。 还公开了表征甲病毒复制子颗粒并利用这些材料用于疫苗和基于基因的治疗剂的方法。