公开(公告)号：US08647864B2

公开(公告)日：2014-02-11

申请号：US12825722

申请日：2010-06-29

Applicant: John M Polo ,  Thomas W Dubensky, Jr. ,  Ilya Frolov ,  Jason P Gardner ,  Gillis Otten ,  Susan Barnett ,  David A Driver

Inventor： John M Polo ,  Thomas W Dubensky, Jr. ,  Ilya Frolov ,  Jason P Gardner ,  Gillis Otten ,  Susan Barnett ,  David A Driver

IPC: C12N15/79 ,  C12N15/01 ,  C12N15/09 ,  A61K48/00

CPC classification number: A61K39/12 ,  A61K2039/5256 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16134 ,  C12N2740/16234 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36145 ,  C12N2810/609

Abstract: Compositions and methods are provided for Eukaryotic Layered Vector Initiation Systems and Alphavirus replicon particles for introducing heterologous sequences into cells for generating immune responses.

Abstract translation: 提供了用于真核分层载体启动系统和甲病毒复制子颗粒的组合物和方法，用于将异源序列引入细胞以产生免疫应答。

公开(公告)号：US20100266631A1

公开(公告)日：2010-10-21

申请号：US12825722

申请日：2010-06-29

Applicant: John Polo ,  Thomas W. Dubensky, JR. ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

Inventor： John Polo ,  Thomas W. Dubensky, JR. ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

IPC: A61K39/12 ,  C12N15/63 ,  C12N7/01 ,  C12N5/071

CPC classification number: A61K39/12 ,  A61K2039/5256 ,  A61K2039/545 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16134 ,  C12N2740/16234 ,  C12N2770/36121 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36145 ,  C12N2810/609

Abstract: Compositions and methods are provided for Eukaryotic Layered Vector Initiation Systems and Alphavirus replicon particles for introducing heterologous sequences into cells for generating immune responses.

Abstract translation: 提供了用于真核分层载体启动系统和甲病毒复制子颗粒的组合物和方法，用于将异源序列引入细胞以产生免疫应答。

公开(公告)号：US5814482A

公开(公告)日：1998-09-29

申请号：US739158

申请日：1996-10-30

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Douglas J. Jolly ,  David A. Driver

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Douglas J. Jolly ,  David A. Driver

IPC: A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12P21/00 ,  C07H21/04

CPC classification number: C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

公开(公告)号：US07771979B2

公开(公告)日：2010-08-10

申请号：US11712044

申请日：2007-02-27

Applicant: John Polo ,  Thomas W. Dubensky, Jr. ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

Inventor： John Polo ,  Thomas W. Dubensky, Jr. ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

IPC: C12N7/08 ,  A61K35/76

CPC classification number: C07K14/005 ,  A61K35/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2740/16122 ,  C12N2770/36143

Abstract: Methods are provided for generating immune responses utilizing alphavirus-based vector systems.

Abstract translation: 提供了使用基于甲病毒的载体系统产生免疫应答的方法。

公开(公告)号：US5843723A

公开(公告)日：1998-12-01

申请号：US739167

申请日：1996-10-30

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC: A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12N5/10

CPC classification number: C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and method,, for utilizing recombinant alphavirus vectors.

Abstract translation: 本发明提供了利用重组甲病毒载体的组合物和方法。

公开(公告)号：US20100173412A1

公开(公告)日：2010-07-08

申请号：US12538494

申请日：2009-08-10

Applicant: Thomas W. Dubensky, JR. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M.W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

Inventor： Thomas W. Dubensky, JR. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M.W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC: C12N5/071

CPC classification number: C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present disclosure provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems

Abstract translation: 本公开提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法

公开(公告)号：US06376236B1

公开(公告)日：2002-04-23

申请号：US09236140

申请日：1999-01-22

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  David A. Driver

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  David A. Driver

IPC: C12N1563

CPC classification number: C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: Disclosed are recombinant alphavirus particles comprising a) an alphavirus vector construct which directs the expression of a heterologous nucleic acid molecule; b) a capsid protein; and c) an envelope glycoprotein from a virus different from said alphavirus vector.

Abstract translation: 公开了重组甲病毒颗粒，其包含a）引导异源核酸分子表达的甲病毒载体构建体; b）衣壳蛋白; 和c）来自不同于所述甲病毒载体的病毒的包膜糖蛋白。

公开(公告)号：US07977091B2

公开(公告)日：2011-07-12

申请号：US12538494

申请日：2009-08-10

Applicant: Thomas W. Dubensky, Jr. ,  John M Polo ,  Carlos E Ibanez ,  Stephen M. W. Chang ,  Douglas J Jolly ,  David A Driver ,  Barbara A Belli

Inventor： Thomas W. Dubensky, Jr. ,  John M Polo ,  Carlos E Ibanez ,  Stephen M. W. Chang ,  Douglas J Jolly ,  David A Driver ,  Barbara A Belli

IPC: C12N15/63 ,  C12Q1/68

CPC classification number: C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present disclosure provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

Abstract translation: 本公开提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US07572628B2

公开(公告)日：2009-08-11

申请号：US10346880

申请日：2003-01-16

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver ,  Barbara A. Belli

IPC: C12N15/63 ,  C12N15/85 ,  A61K48/00 ,  C07H21/04

CPC classification number: C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

Abstract translation: 本发明提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US06333195B1

公开(公告)日：2001-12-25

申请号：US09479776

申请日：2000-01-07

Applicant: James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

Inventor： James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

IPC: C12N15867

CPC classification number: C12N15/86 ,  C12N2740/13043 ,  C12N2740/13052

Abstract: Retroviral vector constructs are described which have a 5′ LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3′ LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.

Abstract translation: 描述了具有5'LTR，tRNA结合位点，包装信号，一个或多个异源序列，第二链合成起始和3'LTR的逆转录病毒载体构建体，其中载体构建体缺乏逆转录病毒gag / pol或env 编码序列。 此外，描述了gag / pol和env表达盒，其中表达盒缺少共同超过8个核苷酸的连续序列。 上述逆转录病毒载体构建体，gag / pol和env表达盒可用于构建排除形成复制能力的病毒的生产细胞系。