公开(公告)号：US20050287637A1

公开(公告)日：2005-12-29

申请号：US11123013

申请日：2005-05-06

申请人： Michael Betenbaugh ,  Shawn Lawrence ,  Yuan Lee ,  Timothy Coleman

发明人： Michael Betenbaugh ,  Shawn Lawrence ,  Yuan Lee ,  Timothy Coleman

IPC分类号： C12N15/52 ,  C12P21/00 ,  C12P21/06 ,  C07H21/04 ,  C12N9/10 ,  C12N9/22

CPC分类号： C12P21/005 ,  C12N15/52

摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

公开(公告)号：US20050153331A1

公开(公告)日：2005-07-14

申请号：US10999956

申请日：2004-12-01

申请人： Timothy Coleman ,  Michael Betenbaugh

发明人： Timothy Coleman ,  Michael Betenbaugh

IPC分类号： A61K48/00 ,  C12N9/12 ,  C12N9/88 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/24

CPC分类号： C12N9/1241 ,  A61K48/00 ,  C07K2319/00 ,  C12N9/88 ,  C12N2799/026

摘要： The present invention relates to novel human glycosylation enzyme polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human glycosylation enzyme polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human glycosylation enzyme polypeptides.

摘要翻译： 本发明涉及新的人糖基化酶多肽和含有编码这些多肽的基因的编码区的分离的核酸。 还提供了用于产生人糖基化酶多肽的载体，宿主细胞，抗体和重组方法。 本发明还涉及用于诊断和治疗与这些新型人糖基化酶多肽有关的病症的诊断和治疗方法。

公开(公告)号：US20120039939A1

公开(公告)日：2012-02-16

申请号：US12937185

申请日：2009-04-10

申请人： Joseph Shiloach ,  Michael Betenbaugh ,  Pratik Jaluria ,  Chia Chu

发明人： Joseph Shiloach ,  Michael Betenbaugh ,  Pratik Jaluria ,  Chia Chu

IPC分类号： A61K39/235 ,  C12N7/02 ,  A61P31/14 ,  A61K39/12 ,  A61P37/04

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16152 ,  C12N2760/16234 ,  C12N2760/16252

摘要： The present invention features methods of producing immunogenic compositions and viruses, methods of treating and preventing viral infection, and methods of producing an immune response using cells that express a polypeptide selected from the group consisting of: cdk13, siat7e, Iama4, cox15, egr1, gas6, map3k9, and gap43, and a virus.

摘要翻译： 本发明的特征在于制备免疫原性组合物和病毒的方法，治疗和预防病毒感染的方法，以及使用表达选自以下的多肽的细胞产生免疫应答的方法：cdk13，siat7e，Iama4，cox15，egr1， gas6，map3k9和gap43，以及病毒。

公开(公告)号：US20060252672A1

公开(公告)日：2006-11-09

申请号：US11397907

申请日：2006-04-05

申请人： Michael Betenbaugh ,  Karthik Viswanathan ,  Sharon Krag ,  Jullian Jones

发明人： Michael Betenbaugh ,  Karthik Viswanathan ,  Sharon Krag ,  Jullian Jones

IPC分类号： A61K38/17 ,  A61K48/00

CPC分类号： A61K38/45 ,  A61K47/543 ,  A61K47/549 ,  A61K48/00 ,  A61K48/005 ,  C12N9/1085 ,  C12N9/1205 ,  C12P21/005

摘要： The level of glycosylation on products produced by a host (such as CHO cells, HEK cells and other mammalian cells, and non-mammalian cells) or patient can be increased by engineering, such as by supplying the host or patient with a gene sequence. For example, the host or patient can be made to produce desirably glycosylated products by increasing one or both of expression of N-glycan substrate containing lipid-liked oligosaccharide and expression of oligosaccharide (OST) transferase.

摘要翻译： 由宿主（如CHO细胞，HEK细胞和其他哺乳动物细胞以及非哺乳动物细胞）或患者产生的产物上的糖基化水平可以通过工程来增加，例如通过向宿主或患者提供基因序列。 例如，通过增加含有脂质寡糖的N-聚糖底物的表达和寡糖（OST）转移酶的表达的一种或两种，可以使宿主或患者制成合适的糖基化产物。

公开(公告)号：US20110200987A1

公开(公告)日：2011-08-18

申请号：US12438706

申请日：2007-08-24

申请人： Joseph Shiloach ,  Pratik Jaluria ,  Michael Betenbaugh

发明人： Joseph Shiloach ,  Pratik Jaluria ,  Michael Betenbaugh

IPC分类号： C12Q1/68 ,  C12N5/00 ,  C12N15/00 ,  C12N15/63 ,  C07H21/00

CPC分类号： C12Q1/6809 ,  C12Q1/6883 ,  C12Q2600/158

摘要： The invention generally provides compositions and methods for modulating cell properties and enhancing recombinant protein yields. In particular, the compositions and methods provide for cells having altered growth characteristics, including altered adhesion, rate of proliferation, growth to particular cell density, and recombinant protein expression level.

摘要翻译： 本发明通常提供用于调节细胞性质并增强重组蛋白质产量的组合物和方法。 特别地，组合物和方法提供具有改变的生长特征的细胞，包括改变的粘附，增殖速率，生长到特定细胞密度和重组蛋白表达水平。

公开(公告)号：US20100167396A1

公开(公告)日：2010-07-01

申请号：US12297620

申请日：2007-04-21

申请人： John Edward Murphy ,  Konstantin Borislavov Konstantinov ,  John Christopher Thrift ,  Tarangsri Nivitchanyong ,  Michael Betenbaugh

发明人： John Edward Murphy ,  Konstantin Borislavov Konstantinov ,  John Christopher Thrift ,  Tarangsri Nivitchanyong ,  Michael Betenbaugh

IPC分类号： C12N5/10

CPC分类号： A61K38/17 ,  C12N5/0018 ,  C12N2501/48 ,  C12N2510/02

摘要： The present invention relates to preventing or delaying programmed cell death by expressing one or more anti-apoptotic polypeptides in a cell expressing recombinant Factor VIII such that programmed cell death in the cell is prevented or delayed. The present invention also relates to increasing production of recombinant Factor VIII by expressing one or more anti-apoptotic polypeptides in a cell such that production of recombinant Factor VIII by the cell is increased. Recombinant cells useful for producing Factor VIII.

摘要翻译： 本发明涉及通过在表达重组因子VIII的细胞中表达一种或多种抗细胞凋亡多肽来防止或延缓程序性细胞死亡，从而阻止或延迟细胞中的程序性细胞死亡。 本发明还涉及通过在细胞中表达一种或多种抗细胞凋亡多肽来增加重组因子VIII的产生，从而增加细胞产生重组因子VIII。 可用于产生因子VIII的重组细胞。