公开(公告)号：US08243266B2

公开(公告)日：2012-08-14

申请号：US12788136

申请日：2010-05-26

申请人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

发明人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

IPC分类号： G01N1/10

CPC分类号： G01N35/00

摘要： A sample observation system and a sample observation process are provided which do not need any special manipulation or treatment to limit the motion of the sample or adjust the orientation of the sample when observing the sample, and enable observation of samples simply from wider direction than ever. A sample observation system of a sample whose vertical direction is defined by gravity, wherein the system at least includes a sample holder having at least one penetrating hole for retaining a liquid containing the sample; an observation unit for observing the sample; and an angle adjusting unit for varying the angle of the observation unit to the direction of the gravity which acts on the sample in a condition in which the sample holder and the observation unit have a fixed relative angle therebetween.

摘要翻译： 提供样品观察系统和样品观察过程，其不需要任何特殊的操作或处理来限制样品的运动或在观察样品时调整样品的取向，并且能够简单地从宽的方向观察样品 。 样品的样品观察系统，其垂直方向由重力限定，其中所述系统至少包括具有至少一个用于保持含有样品的液体的穿透孔的样品保持器; 用于观察样品的观察单元; 以及角度调整单元，用于在样本保持器和观察单元之间具有固定的相对角度的状态下，改变观察单元与作用在样本上的重力方向的角度。

公开(公告)号：US20110243850A1

公开(公告)日：2011-10-06

申请号：US13133381

申请日：2009-12-24

申请人： Taichi Shintou ,  Takeshi Miyazaki ,  Masashi Hirose ,  Taketoshi Okubo ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Taichi Shintou ,  Takeshi Miyazaki ,  Masashi Hirose ,  Taketoshi Okubo ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  C07D417/10 ,  C07D417/14 ,  C07D209/94 ,  C07D403/10 ,  C07D413/10 ,  C07D409/10 ,  C07D401/10 ,  C07D405/10 ,  G01N1/30

CPC分类号： A61K51/0453 ,  A61K49/0002 ,  A61K49/0008 ,  A61K49/0021 ,  A61K49/006 ,  A61K49/10 ,  C07D209/08 ,  C07D209/86 ,  C07D209/94 ,  C07D401/08 ,  C07D403/06 ,  C07D403/08 ,  C07D413/08 ,  C07D417/06 ,  C07D417/14 ,  C09B23/0091 ,  C09B23/04 ,  C09B23/105 ,  G01N33/582 ,  G01N2333/4603 ,  G01N2500/10

摘要： Provided is a novel probe for a biological specimen for labelling by itself and clearly visualizing one of a specific cell and a specific cell organ in a living body, the probe having excellent spectral characteristics and exhibiting excellent storage stability. The probe for a biological specimen contains, as an active agent, at least one kind of compound represented by a general formula (I).

摘要翻译： 本发明提供了一种用于标记本身并且清楚地观察生物体中的特定细胞和特定细胞器官之一的生物样本的新型探针，所述探针具有优异的光谱特性并且显示出优异的储存稳定性。 用于生物样品的探针作为活性剂含有至少一种由通式（I）表示的化合物。

公开(公告)号：US20110236310A1

公开(公告)日：2011-09-29

申请号：US13133357

申请日：2009-12-24

申请人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  C07D221/18 ,  A61K51/04 ,  C07D491/14 ,  C09B1/54 ,  C07D311/82 ,  C07D417/06 ,  A61K51/08

CPC分类号： A61K49/0021 ,  A61K49/0002 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/006 ,  C07D209/70 ,  C07D209/96 ,  C07D215/00 ,  C07D221/14 ,  C07D221/18 ,  C07D263/54 ,  C07D277/34 ,  C07D277/36 ,  C07D277/64 ,  C07D311/82 ,  C07D311/86 ,  C07D405/04 ,  C07D405/06 ,  C07D413/04 ,  C07D413/06 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C07D491/052 ,  C07D491/12 ,  C07D497/10 ,  G01N33/5088 ,  G01N33/582 ,  G01N2333/4603

摘要： The invention provides a labeling composition for an intraocular tissue of a living individual, which specifically labels the intraocular tissue without need of an invasive operation such as exposure of an ocular tissue or injection of a staining agent into the ocular tissue or a nerve tissue linking to the ocular tissue, a method of noninvasively labeling an intraocular tissue of a living individual, and a screening method using the labeling composition for the intraocular tissues. The composition contains a compound capable of labeling at least a photoreceptor cell layer of a retina, wherein the compound is a staining compound having a particular structure as a partial structure thereof.

摘要翻译： 本发明提供了一种用于生物个体的眼内组织的标记组合物，其特异性地标记眼内组织，而不需要诸如眼组织的暴露或注射染色剂的侵入性手术或连接到眼组织的神经组织 眼组织，活体标记眼内组织的方法，以及使用眼内组织标记组合物的筛选方法。 该组合物含有能够至少标记视网膜感光细胞层的化合物，其中该化合物是具有特定结构的染色化合物作为其部分结构。

公开(公告)号：US20110182810A1

公开(公告)日：2011-07-28

申请号：US13084971

申请日：2011-04-12

申请人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

发明人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

IPC分类号： A61K51/04 ,  A61K49/10

CPC分类号： A61K49/0004 ,  A61K49/0032 ,  A61K49/0041 ,  A61K51/0446

摘要： There is provided a central nervous system labelling composition for intranasal administration for the purpose of labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration. Additionally, there is provided a method of non-invasively labelling the central nervous system by way of an administration route that entails little transferability to the entire body. Furthermore, there is provided a screening method using a central nervous system labelling composition for intranasal administration. A central nervous system labelling composition for intranasal administration is characterized by labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration and by containing at least one compound expressed either by the general formula (1) or the general formula (2) shown below as effective component:

摘要翻译： 提供了用于鼻内给药的中枢神经系统标记组合物，用于通过嗅球通过鼻涕管理从嗅觉上皮标记中枢神经系统的目的。 此外，提供了一种通过对整个身体几乎不具有转移性的给药途径非侵入性地标记中枢神经系统的方法。 此外，提供了一种使用中枢神经系统标记组合物进行鼻内给药的筛选方法。 用于鼻内给药的中枢神经系统标记组合物的特征在于通过嗅球标记嗅觉上皮的中枢神经系统，并通过鼻内给药并含有至少一种由通式（1）或 以下所示的通式（2）为有效成分：

公开(公告)号：US20100302534A1

公开(公告)日：2010-12-02

申请号：US12788136

申请日：2010-05-26

申请人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

发明人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

IPC分类号： G01N1/10

CPC分类号： G01N35/00

摘要： A sample observation system and a sample observation process are provided which do not need any special manipulation or treatment to limit the motion of the sample or adjust the orientation of the sample when observing the sample, and enable observation of samples simply from wider direction than ever. A sample observation system of a sample whose vertical direction is defined by gravity, wherein the system at least includes a sample holder having at least one penetrating hole for retaining a liquid containing the sample; an observation unit for observing the sample; and an angle adjusting unit for varying the angle of the observation unit to the direction of the gravity which acts on the sample in a condition in which the sample holder and the observation unit have a fixed relative angle therebetween.

摘要翻译： 提供样品观察系统和样品观察过程，其不需要任何特殊的操作或处理来限制样品的运动或在观察样品时调整样品的取向，并且能够简单地从宽的方向观察样品 。 样品的样品观察系统，其垂直方向由重力限定，其中所述系统至少包括具有至少一个用于保持含有样品的液体的穿透孔的样品保持器; 用于观察样品的观察单元; 以及角度调整单元，用于在样本保持器和观察单元之间具有固定的相对角度的状态下，改变观察单元与作用在样本上的重力方向的角度。

公开(公告)号：US20100166663A1

公开(公告)日：2010-07-01

申请号：US12644472

申请日：2009-12-22

申请人： Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Kohei Watanabe ,  Taichi Shintou ,  Kaoru Takahashi ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Kohei Watanabe ,  Taichi Shintou ,  Kaoru Takahashi ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  A61P27/16

CPC分类号： G01N33/5005 ,  A61K49/0032 ,  A61K49/0041 ,  A61K49/006 ,  C09B11/24 ,  C09B23/0016 ,  C09B23/0058 ,  C09B23/0066 ,  C09B23/0075 ,  C09B23/06 ,  C09B23/105 ,  C09B23/107 ,  G01N33/5044 ,  G01N33/6893 ,  G01N2800/14 ,  G01N2800/20

摘要： Provided are a novel probe for a hair cell for clearly identifying various conditions of a hair cell, and a labelling method for a hair cell using the probe for a hair cell, more particularly, a probe for a hair cell containing, as an active agent, at least one kind selected from staining compounds represented by one of the general formulae (I) and (II), and a labelling method for a hair cell using the probe for a hair cell.

摘要翻译： 本发明提供一种用于毛细胞的新型探针，用于清楚地识别毛细胞的各种条件，以及使用毛细胞探针的毛细胞的标记方法，更具体地，涉及含有作为活性成分的毛细胞的探针 ，选自由通式（I）和（II）之一表示的染色化合物中的至少一种，以及使用毛细胞探针的毛细胞的标记方法。

公开(公告)号：US10227337B2

公开(公告)日：2019-03-12

申请号：US13397759

申请日：2012-02-16

申请人： Toshio Tanaka ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Mie Okano ,  Taichi Shintou ,  Takeshi Miyazaki ,  Yuhei Nishimura ,  Yasuhito Shimada

发明人： Toshio Tanaka ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Mie Okano ,  Taichi Shintou ,  Takeshi Miyazaki ,  Yuhei Nishimura ,  Yasuhito Shimada

IPC分类号： C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C09B23/06 ,  G01N33/50

摘要： Provided is an evaluation probe capable of optically evaluating permeability of a substance through a central nervous system tissue. Also provided is an evaluation method for permeability of a substance through a central nervous system tissue using an evaluation probe for central nervous system permeability. Also provided is a screening method using an evaluation probe for central nervous system permeability. The evaluation probe for central nervous system permeability includes, as an active ingredient, at least one kind of compound represented by the following general formula (1).

公开(公告)号：US20110189096A1

公开(公告)日：2011-08-04

申请号：US13084997

申请日：2011-04-12

申请人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Mie Okano ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

发明人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Mie Okano ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

IPC分类号： A61K49/00 ,  C07D221/18 ,  C07D491/147 ,  C07D221/14 ,  C07D417/10 ,  C07D417/06

CPC分类号： A61K49/0032 ,  A61K49/0039 ,  A61K49/006 ,  C07D417/06 ,  G01N33/50 ,  G01N33/58 ,  G01T1/161

摘要： To provide a central nervous system tissue-labeling composition labeling the central nervous tissue system. Also, another object of the present invention is to provide a method for non-invasively labeling the central nervous tissue system. Further, another object of the present invention is to provide a screening method using the above central nervous system tissue-labeling composition. A central nervous system tissue-labeling composition containing, as an active ingredient, at least one of compounds represented by the general formula (1) or (7).

摘要翻译： 提供标记中枢神经组织系统的中枢神经系统组织标记组合物。 此外，本发明的另一个目的是提供一种非侵入性标记中枢神经组织系统的方法。 此外，本发明的另一个目的是提供一种使用上述中枢神经系统组织标记组合物的筛选方法。 一种中枢神经系统组织标记组合物，含有由通式（1）或（7）表示的至少一种化合物作为活性成分。

公开(公告)号：US20100300547A1

公开(公告)日：2010-12-02

申请号：US12788168

申请日：2010-05-26

申请人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

发明人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

IPC分类号： F17D3/00 ,  B23Q3/00 ,  B65D47/18

CPC分类号： B65D19/002 ,  B65D19/0002 ,  Y10T137/0318

摘要： It is intended to provide an object-holding sheet and an object-treating equipment, which are useful for arranging objects such as biological materials (e.g., cells, embryos or individuals) or particles having the similar size thereto and efficiently conducting procedures such as analysis and fractionation on the objects. The present invention provides an object-holding sheet for holding two or more objects, the object-holding sheet including: a sheet member having two or more through-holes; and an anti-drop member corresponding to each of the holes, wherein the holes have a size that enables only one of the objects to be held, and the anti-drop member is disposed in the vicinity of one opening of each of the holes so as not to permit the object held in each of the holes to pass through the opening.

摘要翻译： 旨在提供一种对象保持用片材和物体治疗装置，其可用于排列诸如生物材料（例如细胞，胚胎或个体）的物体或具有相似尺寸的颗粒，并且有效地进行诸如分析 并对物体进行分馏。 本发明提供一种用于保持两个或多个物体的物体保持片，所述物体保持片包括：具有两个或更多个通孔的片状构件; 以及对应于每个孔的防滴落构件，其中，所述孔具有能够仅保持一个物体的尺寸，并且防滴落构件设置在每个孔的一个开口附近，因此 因为不允许保持在每个孔中的物体通过开口。

公开(公告)号：US08652438B2

公开(公告)日：2014-02-18

申请号：US13084971

申请日：2011-04-12

申请人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

发明人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

IPC分类号： A61K51/00 ,  A61M36/14

CPC分类号： A61K49/0004 ,  A61K49/0032 ,  A61K49/0041 ,  A61K51/0446

摘要： There is provided a central nervous system labelling composition for intranasal administration for the purpose of labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration. Additionally, there is provided a method of non-invasively labelling the central nervous system by way of an administration route that entails little transferability to the entire body. Furthermore, there is provided a screening method using a central nervous system labelling composition for intranasal administration. A central nervous system labelling composition for intranasal administration is characterized by labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration and by containing at least one compound expressed either by the general formula (1) or the general formula (2) shown below as effective component:

摘要翻译： 提供了用于鼻内给药的中枢神经系统标记组合物，用于通过嗅球通过鼻涕管理从嗅觉上皮标记中枢神经系统的目的。 此外，提供了一种通过对整个身体几乎不具有转移性的给药途径非侵入性地标记中枢神经系统的方法。 此外，提供了一种使用中枢神经系统标记组合物进行鼻内给药的筛选方法。 用于鼻内给药的中枢神经系统标记组合物的特征在于通过嗅球标记嗅觉上皮的中枢神经系统，并通过鼻内给药并含有至少一种由通式（1）或 以下所示的通式（2）为有效成分：