公开(公告)号：US11273433B2

公开(公告)日：2022-03-15

申请号：US17177901

申请日：2021-02-17

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: B01J35/00 ,  B33Y80/00 ,  B01J21/08 ,  B01J23/02 ,  B01J23/10 ,  B01J23/30 ,  B01J23/34 ,  B01J23/745 ,  B01J23/755 ,  B01J35/02 ,  B01J37/00 ,  B01J37/02 ,  B01J37/14 ,  C07C2/84 ,  C07C5/48 ,  B33Y10/00 ,  B29C64/165 ,  B28B1/00

Abstract: The disclosure relates to a single-atom-based catalyst system with total-length control of single-atom catalytic sites. The single-atom-based catalyst system comprises at least one catalyst structure comprising a first assembly of a plurality of single-atom-catalyst superparticles. The single-atom-catalyst superparticles comprise a second assembly of a plurality of single-atom-catalyst nanoparticles. The single-atom-based catalyst system has controlled porosity and spatial distribution of active single-atom catalysts from the atomic scale to the macroscopic scale. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

公开(公告)号：US20210139873A1

公开(公告)日：2021-05-13

申请号：US17091735

申请日：2020-11-06

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao ,  Tian Jiang

IPC: C12N9/22 ,  C12N15/01 ,  C12N15/10

Abstract: Disclosed herein are improved nanozymes for targeting RNA. The disclosed nanozymes are synthesized using recombinant RNase A with site-specific cysteine-substituted mutations that can be covalently functionalized with a length-tunable multi-thiol tether and then loaded onto gold particles through multiple gold-sulfur bonds. This new RNase A loading mechanism is site specific, and it allows high-density loading of alkylthiol modified DNA oligonucleotides. The disclosed nanozymes can also include additional capturer strands and/or involve DNA-recombinant-RNase-A unibodies to further increase the nanozyme's enzymatic activity and target selectivity. Also disclosed are functional on-off switchable nanozymes to control nanozyme activity. In some embodiments, the disclosed nanozyme are core-free hollow forms. The removal of the inorganic nanoparticle cores from nanozymes can effectively eliminate the potential long-term toxicity induced by the core, and also creates a cavity for loading and delivery of small molecule drugs.

公开(公告)号：US20190323069A1

公开(公告)日：2019-10-24

申请号：US16366731

申请日：2019-03-27

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: C12Q1/6816 ,  G01N33/58 ,  B01L3/00 ,  G01N33/543

Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can include an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

公开(公告)号：US09291758B2

公开(公告)日：2016-03-22

申请号：US14107148

申请日：2013-12-16

Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Yunwei Charles Cao ,  Tie Wang ,  Xirui Wang

IPC: F21V9/14 ,  G02B5/30 ,  G02C7/12 ,  C08K7/02 ,  B82Y30/00 ,  H01L33/58 ,  C09K11/56 ,  C09K11/88 ,  H01L33/00 ,  H01L33/44 ,  H01L33/50

CPC classification number: G02B5/30 ,  B82Y30/00 ,  C08K7/02 ,  C09K11/565 ,  C09K11/883 ,  H01L33/44 ,  H01L33/50 ,  H01L33/58 ,  Y10T428/298

Abstract: Nanorods assemblies that have lengths in excess of 50 microns to meters are formed from contacting rice-shaped colloidal superparticles that are aligned along the long axis of the colloidal superparticles. The rice-shaped colloidal superparticles are formed from a multiplicity of nanorods with a high degree of association that is end to end to form colloidal superparticles that are in excess of three microns in length and have a length to diameter ratio of about three or more. Methods of preparing the rice-shaped colloidal superparticles employ mixing with an additional ligand to the nanorods to bias the self assembly of the nanorods by solvophobic interactions. Methods of preparing the nanorods assemblies include the infusion of the rice-shaped colloidal superparticles into microchannels patterned on a substrate, wherein the rice-shaped colloidal superparticles' long axes align in the microchannels.

Abstract translation: 长度超过50微米至米的纳米棒组件由与胶体超细颗粒的长轴对准的米状胶体超细颗粒接触形成。 米状胶体超微粒由具有高度结合关系的多个纳米棒形成，其为端到端形成长度超过3微米且长度与直径之比约为3或更大的胶体超细颗粒。 制备米状胶体超微颗粒的方法使用与纳米棒的另外的配体混合以通过疏解相互作用偏置纳米棒的自组装。 制备纳米棒组件的方法包括将米状胶体超级颗粒注入图案化在基底上的微通道，其中米状胶体超微粒的长轴在微通道中对准。

公开(公告)号：US20140319563A1

公开(公告)日：2014-10-30

申请号：US14107148

申请日：2013-12-16

Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Yunwei Charles Cao ,  Tie Wang ,  Xirui Wang

IPC: G02B5/30 ,  H01L33/58

CPC classification number: G02B5/30 ,  B82Y30/00 ,  C08K7/02 ,  C09K11/565 ,  C09K11/883 ,  H01L33/44 ,  H01L33/50 ,  H01L33/58 ,  Y10T428/298

Abstract: Nanorods assemblies that have lengths in excess of 50 microns to meters are formed from contacting rice-shaped colloidal superparticles that are aligned along the long axis of the colloidal superparticles. The rice-shaped colloidal superparticles are formed from a multiplicity of nanorods with a high degree of association that is end to end to form colloidal superparticles that are in excess of three microns in length and have a length to diameter ratio of about three or more. Methods of preparing the rice-shaped colloidal superparticles employ mixing with an additional ligand to the nanorods to bias the self assembly of the nanorods by solvophobic interactions. Methods of preparing the nanorods assemblies include the infusion of the rice-shaped colloidal superparticles into microchannels patterned on a substrate, wherein the rice-shaped colloidal superparticles' long axes align in the microchannels.

Abstract translation: 长度超过50微米至米的纳米棒组件由与胶体超细颗粒的长轴对准的米状胶体超细颗粒接触形成。 米状胶体超微粒由具有高度结合关系的多个纳米棒形成，其为端到端形成长度超过3微米且长度与直径之比约为3或更大的胶体超细颗粒。 制备米状胶体超微颗粒的方法使用与纳米棒的另外的配体混合以通过疏解相互作用偏置纳米棒的自组装。 制备纳米棒组件的方法包括将米状胶体超级颗粒注入图案化在基底上的微通道，其中米状胶体超微粒的长轴在微通道中对准。

公开(公告)号：US12116603B2

公开(公告)日：2024-10-15

申请号：US17091735

申请日：2020-11-06

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao ,  Tian Jiang

IPC: C12N9/22 ,  C12N15/01 ,  C12N15/10

CPC classification number: C12N9/22 ,  C12N15/01 ,  C12N15/102 ,  C12N2310/52 ,  C12Y301/27005

Abstract: Disclosed herein are improved nanozymes for targeting RNA. The disclosed nanozymes are synthesized using recombinant RNase A with site-specific cysteine-substituted mutations that can be covalently functionalized with a length-tunable multi-thiol tether and then loaded onto gold particles through multiple gold-sulfur bonds. This new RNase A loading mechanism is site specific, and it allows high-density loading of alkylthiol modified DNA oligonucleotides. The disclosed nanozymes can also include additional capturer strands and/or involve DNA-recombinant-RNase-A unibodies to further increase the nanozyme's enzymatic activity and target selectivity. Also disclosed are functional on-off switchable nanozymes to control nanozyme activity. In some embodiments, the disclosed nanozyme are core-free hollow forms. The removal of the inorganic nanoparticle cores from nanozymes can effectively eliminate the potential long-term toxicity induced by the core, and also creates a cavity for loading and delivery of small molecule drugs.

公开(公告)号：US10538757B2

公开(公告)日：2020-01-21

申请号：US15042935

申请日：2016-02-12

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao ,  Chen Liu

IPC: C12N9/96 ,  C12N11/14 ,  A61K38/43 ,  C12N11/08

Abstract: Embodiments of the present disclosure provide for nanozymes that can include a therapeutic agent, methods of making nanozymes, methods of using nanozymes, and the like. In some embodiments, the nanozymes can include a shell that can surround a hollow core that can be configured to receive a compound and the shell can include a recognition moiety and an enzyme.

公开(公告)号：US10968474B2

公开(公告)日：2021-04-06

申请号：US16366731

申请日：2019-03-27

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: C12Q1/6816 ,  B01L3/00 ,  G01N33/543 ,  G01N33/58 ,  B01L7/00 ,  G01N35/10

Abstract: Described herein are fluid-manipulation-based devices and methods of use. Fluid manipulations according to devices and methods as described herein can be configured to perform assays on biological samples. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

公开(公告)号：US10301667B2

公开(公告)日：2019-05-28

申请号：US15355306

申请日：2016-11-18

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: B01L3/00 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/58 ,  B01L7/00 ,  G01N35/10

Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

公开(公告)号：US20170138941A1

公开(公告)日：2017-05-18

申请号：US15355306

申请日：2016-11-18

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: G01N33/543 ,  G01N33/58 ,  C12Q1/68 ,  B01L3/00 ,  G01N33/53

CPC classification number: C12Q1/6816 ,  B01L3/502761 ,  B01L7/00 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0424 ,  B01L2400/0487 ,  G01N33/54313 ,  G01N33/587 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2563/137

Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.