公开(公告)号：US5362624A

公开(公告)日：1994-11-08

申请号：US925300

申请日：1992-08-05

申请人： Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg ,  Thomas Geiger ,  Wolf D. Engel

发明人： Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg ,  Thomas Geiger ,  Wolf D. Engel

IPC分类号： G01N33/531 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  G01N33/58 ,  C01N33/576 ,  C01N33/547

CPC分类号： G01N33/57473 ,  G01N33/531 ,  G01N33/54366 ,  G01N33/56988 ,  G01N33/58 ,  Y10S435/82 ,  Y10S436/811

摘要： For the determination of an immunologically detectable substance based on a heterogeneous immunoassay by use of a solid phase on which one of the immunologically active reaction components is bound, a reaction vessel is used as the solid phase on the inner surface of which streptavidin or avidin is bound in such an amount that 0.1 to 2.5 .mu.g are present per ml reaction volume.A suitable reaction vessel for this has optically transparent wall areas which face one another and has avidin or streptavidin coated walls which are at least partially within the inner wall region intended as a receptacle for liquid, wherein the inner space of the container intended as a receptacle for liquid and the respective streptavidin or avidin content of the coating are so matched that 0.1 to 2.5 .mu.g streptavidin or avidin are present per ml reaction volume.

摘要翻译： 为了通过使用其上结合有免疫活性的反应组分的固相来测定基于非均相免疫测定的免疫学可检测物质，使用反应容器作为其内表面上的固相，其中链霉亲和素或抗生物素蛋白为 结合量为每ml反应体积存在0.1至2.5μg。 合适的反应容器具有相互面对的光学透明壁区域，并且具有至少部分位于旨在作为液体容器的内壁区域内的抗生物素蛋白或链霉抗生物素蛋白包被的壁，其中容器的内部空间用作容器 对于液体，并且涂层的各自的链霉抗生物素蛋白或抗生物素蛋白含量如此匹配，每ml反应体积存在0.1至2.5μg链霉亲和素或抗生物素蛋白。

公开(公告)号：USRE34312E

公开(公告)日：1993-07-13

申请号：US796545

申请日：1991-11-21

申请人： Thomas Geiger ,  Wolf D. Engel ,  Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg

发明人： Thomas Geiger ,  Wolf D. Engel ,  Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg

IPC分类号： G01N33/543 ,  G01N33/576 ,  G01N33/68

CPC分类号： G01N33/6854 ,  G01N33/5764 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/827

摘要： The present invention provides a process for the determination of an antibody by incubation with three different reagents R.sub.1, R.sub.2 and R.sub.3, of which R.sub. and R.sub.3 are present in liquid phase and are bindable with the antibody, R.sub.2 is present bound to a solid phase and is bindable with R.sub.1 and R.sub.3 carries a label, separation of the solid phase from the liquid phase and measurement of the label in the solid phase, wherein as R.sub.1 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a reaction component of a specific binding system, as R.sub.3 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a label and as R.sub.2 there used the other binding component of the specific binding system. The present invention also provides a composition for the determination of an antibody, wherein it contains two different soluble reagents R.sub.1 and R.sub.3 specifically bindable with the antibody, of which R.sub.1 carries a reaction component of a specific binding system and R.sub.3 carries a label and also a receptor R.sub.2 which is present in solid phase and is bindable with the receptor R.sub.1, the insoluble receptor R.sub.2 and the soluble receptor R.sub.1 being present physically separated from one another.

公开(公告)号：US5776702A

公开(公告)日：1998-07-07

申请号：US271855

申请日：1994-07-07

申请人： Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg

发明人： Urban Schmitt ,  Eberhard Maurer ,  Wolfgang Rudinger ,  Rolf Deeg

IPC分类号： G01N33/531 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  G01N33/58 ,  G01N33/547

CPC分类号： G01N33/57473 ,  G01N33/531 ,  G01N33/54366 ,  G01N33/56988 ,  G01N33/58 ,  Y10S435/82 ,  Y10S436/811

摘要： For the determination of an immunologically detectable substance based on a heterogeneous immunoassay by use of a solid phase on which one of the immunologically active reaction components is bound, a reaction vessel is used as the solid phase on the inner surface of which streptavidin or avidin is bound in such an amount that 0.1 to 2.5 .mu.g are present per ml reaction volume. A suitable reaction vessel for this has optically transparent wall areas which face one another and has avidin or streptavidin coated walls which are at least partially within the inner wall region intended as a receptacle for liquid, wherein the inner space of the container intended as a receptacle for liquid and the respective streptavidin or avidin content of the coating are so matched that 0.1 to 2.5 .mu.g streptavidin or avidin are present per ml reaction volume.

摘要翻译： 为了通过使用其上结合有免疫活性的反应组分的固相来测定基于非均相免疫测定的免疫学可检测物质，使用反应容器作为其内表面上的固相，其中链霉亲和素或抗生物素蛋白为 结合量为每ml反应体积存在0.1至2.5μg。 合适的反应容器具有相互面对的光学透明壁区域，并且具有至少部分位于旨在作为液体容器的内壁区域内的抗生物素蛋白或链霉抗生物素蛋白包被的壁，其中容器的内部空间用作容器 对于液体，并且涂层的各自的链霉抗生物素蛋白或抗生物素蛋白含量如此匹配，每ml反应体积存在0.1至2.5μg链霉亲和素或抗生物素蛋白。

公开(公告)号：US5277589A

公开(公告)日：1994-01-11

申请号：US663759

申请日：1991-03-01

申请人： Urban Schmitt ,  Wolfgang Rudinger ,  Gertraud Ehrlich-Weinreich

发明人： Urban Schmitt ,  Wolfgang Rudinger ,  Gertraud Ehrlich-Weinreich

IPC分类号： G01N33/68 ,  G01N33/543 ,  C07K13/00 ,  C12Q1/00 ,  G01N33/564

CPC分类号： G01N33/686 ,  Y10S530/81 ,  Y10S530/866

摘要： For the determination of antibodies based on an immunoassay technique by incubation with at least three receptors R.sub.1, R.sub.2 and R.sub.3 which are present dissolved in a liquid phase and of which R.sub.1 is an antigen which is capable of being specifically bound to the antibody to be determined, R.sub.2 mediates the binding to the solid phase and R.sub.3 carries a label, separation of the complex which forms from the solution by binding to a solid phase and measurement of the label in one of the phases, a conjugate is used as the receptor R.sub.2 composed of a receptor capable of specific binding to R.sub.1 and a substance S.sub.1, which can be specifically bound, and a conjugate of a receptor which can specifically bind to R.sub.1 and a label is used as R.sub.3, wherein the immobilization of the complex which forms is mediated by binding to a component of the solid phase which can specifically bind S.sub.1.

摘要翻译： 对于基于免疫测定技术的抗体的测定，其通过与存在于​​液相中的至少三种受体R1，R2和R3温育，其中R1是能够特异性结合待测定的抗体的抗原 ，R2介导与固相的结合，R3携带标记，通过结合固相分离与溶液形成的络合物，并在其中一个相中测量标记，使用缀合物作为受体R2组成 可以特异性结合R1的受体和可以特异性结合的物质S1和可以特异性结合R1的受体的结合物和标记物用作R3，其中形成的络合物的固定被介导 通过与可以特异性结合S1的固相的组分结合。

公开(公告)号：US4438204A

公开(公告)日：1984-03-20

申请号：US426510

申请日：1982-09-29

申请人： Rolf Deeg ,  Urban Schmitt ,  Joachim Ziegenhorn

发明人： Rolf Deeg ,  Urban Schmitt ,  Joachim Ziegenhorn

IPC分类号： G01N33/52 ,  G01N21/78 ,  G01N30/88 ,  G01N33/53 ,  G01N33/66 ,  G01N33/72

CPC分类号： G01N33/723 ,  Y10T436/107497

摘要： A process for the determination of glycosilated hemoglobin in a blood sample comprises liberating glycosilated and non-glycosilated hemoglobin from the erythrocytes by chemical or physical treatment; converting, if desired, the hemoglobin into methemoglobin, and thereafter differentiating the glycosilated and non-glycosilated hemoglobin portion using its reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated hemoglobin portion.The present invention also provides a reagent for the determination of glycosilated hemoglobin in a blood sample, as well as a reagent for the differentiation of glycosilated and non-glycosilated hemoglobin, comprising haptoglobin.

摘要翻译： 用于测定血液样品中糖基化血红蛋白的方法包括通过化学或物理处理从红细胞释放糖基化和非糖基化血红蛋白; 如果需要，将血红蛋白转化成高铁血红蛋白，然后使用其与触珠蛋白的反应来分化糖基化和非糖基化血红蛋白部分，并测定糖基化和/或非糖基化血红蛋白部分。 本发明还提供了用于测定血液样品中的糖基化血红蛋白的试剂，以及包含触珠蛋白的糖基化和非糖化血红蛋白的分化试剂。

公开(公告)号：US4695552A

公开(公告)日：1987-09-22

申请号：US601801

申请日：1984-04-19

申请人： Urban Schmitt ,  Rolf Deeg ,  Joachim Ziegenhorn

发明人： Urban Schmitt ,  Rolf Deeg ,  Joachim Ziegenhorn

IPC分类号： C12Q1/28 ,  G01N33/72

CPC分类号： C12Q1/28 ,  G01N33/725 ,  C12Q2326/30 ,  Y10T436/25125

摘要： The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured.The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin.Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.

摘要翻译： 本发明提供了一种通过利用游离血红蛋白和结合血红蛋白的不同过氧化物特性在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法，其中，对于选择性抑制游离血红蛋白的过氧化物酶活性， 添加洗涤剂，并测量反应混合物的过氧化物的残留活性。 本发明还提供了一种用于进行该方法的试剂，其中除了确定过氧化物酶活性所需的物质外，还含有用于抑制游离血红蛋白的过氧化物酶活性的洗涤剂。 此外，本发明提供了测定样品的触珠蛋白含量的方法，以及测定样品中糖基化血红蛋白的方法。

公开(公告)号：US06187270B1

公开(公告)日：2001-02-13

申请号：US08499078

申请日：1995-07-06

申请人： Urban Schmitt ,  Eberhard Maurer ,  Gunter Pappert

发明人： Urban Schmitt ,  Eberhard Maurer ,  Gunter Pappert

IPC分类号： G01N3510

CPC分类号： B03C1/288 ,  G01N35/0098 ,  G01N35/1065 ,  G01N2035/1055 ,  G01N2035/1062 ,  Y10S436/807 ,  Y10T436/11 ,  Y10T436/119163 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

摘要： The invention addresses a device for separating magnetic microparticles in a liquid with the aid of a magnetic field. During the separation procedure, the liquid containing the microparticles is located in the tip of the pipette. Moreover, the invention also addresses a method for separating microparticles, and a method for washing microparticles.

摘要翻译： 本发明涉及借助于磁场分离液体中的磁性微粒的装置。 在分离过程中，含有微粒的液体位于移液管的尖端。 此外，本发明还涉及一种分离微粒的方法，以及一种洗涤微粒的方法。

公开(公告)号：US09261510B2

公开(公告)日：2016-02-16

申请号：US12104751

申请日：2008-04-17

申请人： Christian Schotz ,  Elke Faatz ,  Peter Schaarschmidt ,  Urban Schmitt

发明人： Christian Schotz ,  Elke Faatz ,  Peter Schaarschmidt ,  Urban Schmitt

IPC分类号： C07K14/20 ,  C07K14/245 ,  C07K14/45 ,  C07K14/47 ,  C12N15/62 ,  G01N33/68

CPC分类号： G01N33/6854 ,  C07K14/20 ,  C07K14/245 ,  C07K14/45 ,  C07K14/47 ,  C07K2319/70 ,  C12N15/62 ,  C12N2710/16122 ,  C12N2710/16134 ,  G01N2333/02 ,  G01N2333/035 ,  G01N2333/04 ,  G01N2333/045 ,  G01N2333/05 ,  G01N2333/19 ,  G01N2333/45

摘要： The present invention relates to fusion proteins suitable as test antigens in the detection of infections with pathogens, particularly of primary infections with pathogens. Further, the invention relates to methods for detecting and differentially determining antibodies, particularly IgM antibodies resulting from an infection with a pathogenic organism. Furthermore, test reagents for carrying out these methods are provided.

摘要翻译： 本发明涉及适合作为检测抗原的融合蛋白，用于检测病原体的感染，特别是病原体的初次感染。 此外，本发明涉及用于检测和差异测定抗体的方法，特别是由致病性生物体的感染产生的IgM抗体。 此外，提供了用于实施这些方法的测试试剂。

公开(公告)号：US08790659B2

公开(公告)日：2014-07-29

申请号：US13569353

申请日：2012-08-08

申请人： Christian Scholz ,  Elke Faatz ,  Urban Schmitt ,  Peter Schaarschmidt

发明人： Christian Scholz ,  Elke Faatz ,  Urban Schmitt ,  Peter Schaarschmidt

IPC分类号： A61K39/245

CPC分类号： C12N15/62 ,  C12N9/90 ,  G01N33/536 ,  G01N33/54393 ,  Y02A50/57

摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.

摘要翻译： 公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞 。 还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。 本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。

公开(公告)号：US06417331B1

公开(公告)日：2002-07-09

申请号：US09368772

申请日：1999-08-05

申请人： Eberhard Kopetzki ,  Rainer Müller ,  Richard Engh ,  Urban Schmitt ,  Arno Deger ,  Hans Brandstetter

发明人： Eberhard Kopetzki ,  Rainer Müller ,  Richard Engh ,  Urban Schmitt ,  Arno Deger ,  Hans Brandstetter

IPC分类号： C07K1400

CPC分类号： G01N33/5306 ,  A61K38/00 ,  C07K14/36 ,  C07K14/465

摘要： The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.

摘要翻译： 本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白，其对生物素的结合亲和力降低，以及它们在测定分析物的方法中用作干扰消除试剂。 在诊断测试中，例如免疫测定和核酸杂交测定。 此外，本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白作为能够再生以结合生物素的体系的用途。 用于分析生物素化分子，用于检查受体配体相互作用以及生物素化分子的亲和纯化。