Swellable (meth)acrylate surfaces for culturing cells in chemically defined media
    8.
    发明授权
    Swellable (meth)acrylate surfaces for culturing cells in chemically defined media 有权
    可溶胀的(甲基)丙烯酸酯表面用于在化学上限定的培养基中培养细胞

    公开(公告)号:US08329469B2

    公开(公告)日:2012-12-11

    申请号:US12362974

    申请日:2009-01-30

    IPC分类号: C12N5/00 C12N11/00

    摘要: Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译: 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的(甲基)丙烯酸酯层和与可溶胀的(甲基)丙烯酸酯层共轭的肽。 可溶胀的(甲基)丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯,2-羧基丙烯酸烯丙酯和四(乙二醇)二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列,其中n是0至3的整数,并且其中Xaa是任何氨基酸。 此外,本文公开了可通过γ辐射灭菌的可溶胀(甲基)丙烯酸酯合成表面。

    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA
    9.
    发明申请
    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 有权
    用于化学定义培养基培养细胞的(甲基)丙烯酸酯表面

    公开(公告)号:US20090191632A1

    公开(公告)日:2009-07-30

    申请号:US12362974

    申请日:2009-01-30

    IPC分类号: C12N5/02

    摘要: Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译: 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的(甲基)丙烯酸酯层和与可溶胀(甲基)丙烯酸酯层共轭的肽。 可溶胀的(甲基)丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯,2-羧基丙烯酸烯丙酯和四(乙二醇)二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列,其中n是0至3的整数,并且其中Xaa是任何氨基酸。 此外,本文公开了可通过γ辐射灭菌的可溶胀(甲基)丙烯酸酯合成表面。

    Poly (1-trimethysilyl-1-propyne) membrane regeneration process
    10.
    发明授权
    Poly (1-trimethysilyl-1-propyne) membrane regeneration process 失效
    聚(1-三甲基甲硅烷基-1-丙炔)膜再生过程

    公开(公告)号:US06423119B1

    公开(公告)日:2002-07-23

    申请号:US09611742

    申请日:2000-07-07

    IPC分类号: B01D6136

    摘要: A novel process for the regeneration of PTMSP membranes is disclosed. The regeneration process includes washing a fouled PTMSP membrane with a solution containing at least about 50% butanol in water for a time sufficient to increase the flux and/or selectivity of the membrane. This regeneration process with a butanol containing solution significantly improves both the flux and selectivity of the PTMSP membrane and can regenerate the flux of the PTMSP membrane to the level of a previously unused membrane.

    摘要翻译: 公开了一种用于PTMSP膜再生的新方法。 再生方法包括用在水中含有至少约50%丁醇的溶液洗涤污垢的PTMSP膜足以增加膜的通量和/或选择性的时间。 用含丁醇的溶液的这种再生过程显着地改善了PTMSP膜的通量和选择性,并且可以将PTMSP膜的通量再生成先前未使用的膜的水平。