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    Nematode-resistant transgenic plants
    3.
    发明授权
    Nematode-resistant transgenic plants 失效
    线虫抗性转基因植物

    公开(公告)号：US6008436A

    公开(公告)日：1999-12-28

    申请号：US654025

    申请日：1996-05-23

    申请人： Mark A. Conkling ,  Charles H. Opperman ,  Gregoria N. Acedo ,  Wen Song

    发明人： Mark A. Conkling ,  Charles H. Opperman ,  Gregoria N. Acedo ,  Wen Song

    IPC分类号： A01H5/02 ,  C07K14/415 ,  C12N15/82 ,  C12N15/00 ,  C12N15/29 ,  A01H5/00

    CPC分类号： C07K14/415 ,  A01H5/02 ,  C12N15/8285

    摘要： Nematode-resistant transgenic plants are disclosed. The plants comprise plant cells containing a DNA construct comprising a transcription cassette, which construct comprises, in the 5' to 3' direction, a promoter operable in the plant cells, and a DNA comprising at least a portion of a DNA sequence encoding a nematode-inducible transmembrane pore protein in either the sense or antisense orientation. Intermediates for producing the same along with methods of making and using the same are also disclosed. In an alternate embodiment of the invention, the sense or antisense DNA is replaced with a DNA encoding an enzymatic RNA molecule directed against the mRNA transcript of a DNA sequence encoding a nematode-inducible transmembrane pore protein.

    摘要翻译： 披露了抗线虫的转基因植物。 植物包含含有包含转录盒的DNA构建体的植物细胞，该构建体在5'至3'方向上包含在植物细胞中可操作的启动子，以及包含编码线虫的DNA序列的至少一部分的DNA 有意义或反义方向的诱导性跨膜孔蛋白。 还公开了用于制备该介质的中间体及其制备和使用方法。 在本发明的替代实施方案中，有义或反义DNA被编码针对编码线虫诱导型跨膜孔蛋白的DNA序列的mRNA转录物的酶RNA分子的DNA替代。

    Root cortex specific gene promoter
    10.
    发明授权
    Root cortex specific gene promoter 失效
    根皮质特异性基因启动子

    公开(公告)号：US5837876A

    公开(公告)日：1998-11-17

    申请号：US508786

    申请日：1995-07-28

    申请人： Mark A. Conkling ,  Nandini Mendu ,  Wen Song

    发明人： Mark A. Conkling ,  Nandini Mendu ,  Wen Song

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12N15/32

    CPC分类号： C12N15/8227

    摘要： An isolated DNA molecule which comprises a DNA promoter sequence which directs root cortex specific transcription of a downstream heterologous DNA segment in a plant cell. A DNA construct which comprises an expression cassette comprising, in the 5' to 3' direction, a promoter of the present invention and a heterologous DNA segment positioned downstream from the promoter and operatively associated therewith. Transformed plants, such as tobacco plants, comprise transformed plant cells containing a heterologous DNA construct comprising an expression cassette as described above.

    摘要翻译： 分离的DNA分子，其包含指导植物细胞中下游异源DNA区段的根皮质特异性转录的DNA启动子序列。 一种DNA构建体，其包含表达盒，其在5'至3'方向上包含本发明的启动子和位于启动子下游并与其可操作地相关联的异源DNA区段。 转化植物，如烟草植物，包含含有如上所述的表达盒的异源DNA构建体的转化植物细胞。