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    Methods and compositions for gene silencing
    6.
    发明申请
    Methods and compositions for gene silencing 审中-公开
    用于基因沉默的方法和组合物

    公开(公告)号：US20070130653A1

    公开(公告)日：2007-06-07

    申请号：US11455073

    申请日：2006-06-16

    申请人： David Baulcombe ,  Enno Krebbers ,  William Hitz ,  Kimberly Glassman ,  Milo Aukerman ,  Robert Williams ,  Byung-Chun Yoo

    发明人： David Baulcombe ,  Enno Krebbers ,  William Hitz ,  Kimberly Glassman ,  Milo Aukerman ,  Robert Williams ,  Byung-Chun Yoo

    IPC分类号： A01H1/00 ,  C07H21/02 ,  C12N15/82 ,  C07H21/04 ,  C12N5/04

    CPC分类号： C12N15/8218 ,  C07H21/02 ,  C07H21/04 ,  C12N2310/14

    摘要： Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.

    摘要翻译： 提供了用于降低生物体中靶多核苷酸表达水平的方法和组合物。 所述方法和组合物通过表达嵌合多核苷酸来选择性沉默靶多核苷酸，所述嵌合多核苷酸包含可操作地连接到对应于待沉默的基因或基因的全部或部分的序列的sRNA（触发序列）的靶。 以这种方式，沉默的最终靶标是其中表达嵌合多核苷酸的生物体中的内源基因。 在另一个实施方案中，miRNA靶是异源miRNA或siRNA的靶标，其后者在合适的发育阶段在细胞中共表达，以在需要时和期望时提供最终靶的沉默。 在另一个实施方案中，最终靶可以是在含有嵌合基因或基因的生物体上进食的第二生物体如植物害虫中的基因。 组合物还包含载体，种子，谷粒，细胞和生物，包括植物和植物细胞，其包含本发明的嵌合多核苷酸。

    Plant sorbitol biosynthetic enzymes
    9.
    发明申请
    Plant sorbitol biosynthetic enzymes 失效
    植物山梨醇生物合成酶

    公开(公告)号：US20050042722A1

    公开(公告)日：2005-02-24

    申请号：US10852073

    申请日：2004-05-24

    申请人： Omolayo Famodu ,  Emil Orozco ,  J. Rafalski ,  William Hitz

    发明人： Omolayo Famodu ,  Emil Orozco ,  J. Rafalski ,  William Hitz

    IPC分类号： C12N9/04 ,  C12N15/82 ,  C12P7/18 ,  C07H21/04 ,  C12N9/02

    CPC分类号： C12N9/0006 ,  C12N15/8245 ,  C12P7/18

    摘要： This invention relates to an isolated nucleic acid fragment encoding a sorbitol biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sorbitol biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sorbitol biosynthetic enzyme in a transformed host cell.

    摘要翻译： 本发明涉及编码山梨糖醇生物合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分山梨醇生物合成酶的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中山梨糖醇生物合成酶水平的改变 。