公开(公告)号：US20060078540A1

公开(公告)日：2006-04-13

申请号：US10508350

申请日：2003-03-18

申请人： William Warren ,  Nir Hacohen ,  Lan Chen ,  Darell Irvine ,  Anatoly Kachurin ,  Russell Higbee ,  Qian Huang

发明人： William Warren ,  Nir Hacohen ,  Lan Chen ,  Darell Irvine ,  Anatoly Kachurin ,  Russell Higbee ,  Qian Huang

IPC分类号： C12N5/08 ,  A61K48/00 ,  A61K35/14

CPC分类号： C12N5/0697 ,  A61K2039/5154 ,  C12N5/0639 ,  C12N2502/11

摘要： The present invention features dendritic cell nodes that can be used to vaccinate subjects against pathogens and to modulate a subject's immune system to treat or prevent various diseases and conditions.

摘要翻译： 本发明具有树突细胞节点，其可用于针对病原体接种受试者并调节受试者的免疫系统以治疗或预防各种疾病和病症。

公开(公告)号：US20050282148A1

公开(公告)日：2005-12-22

申请号：US11116234

申请日：2005-04-28

申请人： William Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell Irvine ,  Gwendalyn Randolph ,  Nir Hacohen ,  Bruce Torbett

发明人： William Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell Irvine ,  Gwendalyn Randolph ,  Nir Hacohen ,  Bruce Torbett

IPC分类号： C12N5/071 ,  C12Q1/00 ,  G01N33/50 ,  C12N5/08

CPC分类号： G01N33/5008 ,  C12N5/0697 ,  C12N5/0698 ,  C12N2501/21 ,  C12N2502/094 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1157 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5088 ,  G01N2500/10

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

公开(公告)号：US20110171689A1

公开(公告)日：2011-07-14

申请号：US12725698

申请日：2010-03-17

申请人： William L. Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell J. Irvine ,  Gwendalyn J. Randolph ,  Nir Hacohen ,  Bruce Torbett

发明人： William L. Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell J. Irvine ,  Gwendalyn J. Randolph ,  Nir Hacohen ,  Bruce Torbett

IPC分类号： C12P21/00

CPC分类号： G01N33/5008 ,  C12N5/0697 ,  C12N5/0698 ,  C12N2501/21 ,  C12N2502/094 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1157 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5088 ,  G01N2500/10

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

公开(公告)号：US07855074B2

公开(公告)日：2010-12-21

申请号：US11116234

申请日：2005-04-28

申请人： William L. Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell J. Irvine ,  Gwendalyn J. Randolph ,  Nir Hacohen ,  Bruce Torbett

发明人： William L. Warren ,  Heather Fahlenkamp ,  Russell Higbee ,  Anatoly Kachurin ,  Conan Li ,  Mike Nguyen ,  Robert Parkhill ,  Guzman Sanchez-Schmitz ,  Darrell J. Irvine ,  Gwendalyn J. Randolph ,  Nir Hacohen ,  Bruce Torbett

IPC分类号： C12N5/08

CPC分类号： G01N33/5008 ,  C12N5/0697 ,  C12N5/0698 ,  C12N2501/21 ,  C12N2502/094 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1157 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5088 ,  G01N2500/10

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

公开(公告)号：US20070218054A1

公开(公告)日：2007-09-20

申请号：US11642938

申请日：2006-12-21

申请人： Selva Sukumar ,  Mohey Eldin El Shikh ,  John Tew ,  Guzman Sanchez-Schmitz ,  Donald Drake ,  Luis Mosquera ,  Eric Mishkin ,  Anatoly Kachurin ,  Russell Higbee ,  Conan Li ,  William Warren ,  Heather Fahlenkamp

发明人： Selva Sukumar ,  Mohey Eldin El Shikh ,  John Tew ,  Guzman Sanchez-Schmitz ,  Donald Drake ,  Luis Mosquera ,  Eric Mishkin ,  Anatoly Kachurin ,  Russell Higbee ,  Conan Li ,  William Warren ,  Heather Fahlenkamp

IPC分类号： A61K39/395 ,  G01N33/567 ,  C12P21/04 ,  C12N5/08

CPC分类号： C07K16/44 ,  C12N5/0639 ,  C12N5/0651 ,  C12N5/0697 ,  C12N2501/052 ,  C12N2501/21 ,  C12N2501/2304 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1192 ,  C12N2502/28 ,  C12N2503/04 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2533/92 ,  C12N2535/10 ,  G01N33/5047 ,  G01N33/5076 ,  G01N33/5088

摘要： The present invention incorporates germinal centers (GCs) into three-dimensional (3D) engineered tissue constructs (ETCs). In an embodiment, we have incorporated the GC in the design of an artificial immune system (AIS) to examine immune responses to vaccines and other compounds. Development of an in vitro GC adds functionality to an AIS, in that it enables generation of an in vitro human humoral response by human B lymphocytes that is accurate and reproducible, without using human subjects. The invention also permits evaluation of, for example, vaccines, allergens, and immunogens, and activation of human B cells specific for a given antigen, which can then be used to generate human antibodies. In an embodiment of the present invention the function of the in vitro GC is enhanced by placing FDCs and other immune cells in a 3D ETC; FDCs appear more effective over a longer time (antibody production is sustained for up to about 14 days.

摘要翻译： 本发明将生发中心（GC）纳入三维（3D）工程组织构建体（ETC）中。 在一个实施方案中，我们已经将GC并入人造免疫系统（AIS）的设计中，以检查对疫苗和其他化合物的免疫应答。 体外GC的开发为AIS增加了功能，因为它能够在不使用人类受试者的情况下产生准确和可重复的人B淋巴细胞的体外人体液反应。 本发明还允许评估例如疫苗，过敏原和免疫原，以及对给定抗原特异性的人B细胞的活化，然后可以将其用于产生人抗体。 在本发明的一个实施方案中，通过将FDC和其他免疫细胞放置在3D ETC中来增强体外GC的功能; FDC在更长时间内看起来更有效（抗体产生持续长达约14天。

公开(公告)号：US20070141552A1

公开(公告)日：2007-06-21

申请号：US11594171

申请日：2006-11-08

申请人： William Warren ,  Robert Parkhill ,  Michael Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake ,  Anatoly Kachurin ,  David Moe

发明人： William Warren ,  Robert Parkhill ,  Michael Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake ,  Anatoly Kachurin ,  David Moe

IPC分类号： C12Q1/00 ,  C12N5/08

CPC分类号： G01N33/5088 ,  G01N33/5047

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

公开(公告)号：US08647837B2

公开(公告)日：2014-02-11

申请号：US12173921

申请日：2008-07-16

申请人： Ayesha Mahmood ,  Anatoly Kachurin ,  William Warren ,  Russell Higbee

发明人： Ayesha Mahmood ,  Anatoly Kachurin ,  William Warren ,  Russell Higbee

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12Q1/02

CPC分类号： A61K9/70 ,  A61K35/17 ,  A61K35/36 ,  C12N5/0688 ,  C12N2502/28 ,  C12N2533/54 ,  G01N33/5082 ,  G01N2800/12

摘要： The present invention comprises artificial tissue constructs that serve as in vitro models of mammalian lung tissue. The artificial tissue constructs of the present invention comprise functionally equivalent in vitro tissue scaffolds that enable immunophysiological function of the lung. The constructs can serve as novel platforms for the study of lung diseases (e.g., interstitial lung diseases, fibrosis, influenza, RSV) as well as smoke- and smoking-related diseases. The artificial tissue constructs of the present invention comprise the two components of alveolar tissue, epithelial and endothelial cell layers.

摘要翻译： 本发明包括用作哺乳动物肺组织的体外模型的人造组织构建体。 本发明的人造组织构建体包括能够实现肺的免疫生理功能的功能上等同的体外组织支架。 这些构建体可以作为研究肺部疾病（例如间质性肺疾病，纤维化，流感，RSV）以及吸烟和吸烟相关疾病的新平台。 本发明的人造组织构建体包含肺泡组织，上皮和内皮细胞层的两个组分。

公开(公告)号：US20060270029A1

公开(公告)日：2006-11-30

申请号：US11375033

申请日：2006-03-15

申请人： William Warren ,  Robert Parkhill ,  Michael Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake ,  Anatoly Kachurin ,  David Moe

发明人： William Warren ,  Robert Parkhill ,  Michael Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake ,  Anatoly Kachurin ,  David Moe

IPC分类号： C12M1/34 ,  C12N5/08 ,  C12N5/06

CPC分类号： C12N5/0697 ,  B01L3/5027 ,  C12N2501/21 ,  C12N2502/094 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1192 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5082 ,  G01N33/5088

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

公开(公告)号：US20050244505A1

公开(公告)日：2005-11-03

申请号：US11008936

申请日：2004-12-13

申请人： Russell Higbee ,  Glen Barber ,  Anatoly Kachurin ,  Olga Kachurina ,  Heather Gappa-Fahlekamp ,  William Warren ,  Siddharth Balachandran ,  Emmanuel Thomas ,  Robert Parkhill

发明人： Russell Higbee ,  Glen Barber ,  Anatoly Kachurin ,  Olga Kachurina ,  Heather Gappa-Fahlekamp ,  William Warren ,  Siddharth Balachandran ,  Emmanuel Thomas ,  Robert Parkhill

IPC分类号： A61K9/14 ,  A61K9/16 ,  A61K39/00 ,  A61K39/39 ,  A61K47/36 ,  A61K47/48 ,  A61K48/00 ,  C12N15/113 ,  C12N15/117 ,  C12P21/06 ,  C12N15/87

CPC分类号： C12N15/117 ,  A61K9/1647 ,  A61K9/1652 ,  A61K9/167 ,  A61K39/39 ,  A61K47/36 ,  A61K47/59 ,  A61K47/593 ,  A61K47/61 ,  A61K47/645 ,  A61K47/6927 ,  A61K2039/55555 ,  A61K2039/55561 ,  A61K2039/55583 ,  C12N15/1135 ,  C12N2310/13 ,  C12N2310/17 ,  C12N2310/351 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39

摘要： The present invention directs to compositions and methods for modulating immune system. One aspect of the present invention relates to a composition comprising FADD-dependent signaling pathway modulators. Another aspect of the present invention relates to biodegradable microparticles, such as a chitosan microparticle, or PLGA/PEI microparticle, designed to deliver nucleic acids and/or proteins, such as FADD-dependent signaling pathway modulators, to boost different pathways of an immune response. Another aspect of the present invention relates to the method of making biodegradable microparticles. The further aspect of the present invention relates to the use of the chitosan and other polycationic microparticles to deliver FADD-dependent signaling pathway modulators to modulate immune system for the prevention and/or treatment infectious diseases and cancers.

摘要翻译： 本发明涉及用于调节免疫系统的组合物和方法。 本发明的一个方面涉及包含FADD依赖性信号通路调节剂的组合物。 本发明的另一方面涉及可生物降解的微粒，例如壳聚糖微粒或PLGA / PEI微粒，其设计用于递送核酸和/或蛋白质，例如FADD依赖性信号通路调节剂，以促进免疫应答的不同途径 。 本发明的另一方面涉及制造可生物降解的微粒的方法。 本发明的另一方面涉及壳聚糖和其它聚阳离子微粒用于递送FADD依赖性信号通路调节剂以调节用于预防和/或治疗感染性疾病和癌症的免疫系统的用途。

公开(公告)号：US08298823B2

公开(公告)日：2012-10-30

申请号：US12730899

申请日：2010-03-24

申请人： William L. Warren ,  Robert Parkhill ,  Michael N. Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake, III ,  Anatoly Kachurin ,  David Moe

发明人： William L. Warren ,  Robert Parkhill ,  Michael N. Nguyen ,  Guzman Sanchez-Schmitz ,  Heather Fahlenkamp ,  Russell Higbee ,  Donald Drake, III ,  Anatoly Kachurin ,  David Moe

IPC分类号： C12N5/16 ,  C12N5/02

CPC分类号： C12N5/0697 ,  B01L3/5027 ,  C12N2501/21 ,  C12N2502/094 ,  C12N2502/11 ,  C12N2502/1107 ,  C12N2502/1114 ,  C12N2502/1121 ,  C12N2502/1192 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2531/00 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5082 ,  G01N33/5088

摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.

摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。