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15 条记录 (耗时 0.041 秒)

    Methods for the activation of proliferation of animal cells
    1.
    发明授权
    Methods for the activation of proliferation of animal cells 失效
    激活动物细胞增殖的方法

    公开(公告)号：US5811299A

    公开(公告)日：1998-09-22

    申请号：US385142

    申请日：1995-02-07

    申请人： Wolfgang A. Renner ,  Hans M. Eppenberger ,  James E. Bailey

    发明人： Wolfgang A. Renner ,  Hans M. Eppenberger ,  James E. Bailey

    IPC分类号： C07K14/47 ,  C12N5/06 ,  C12N5/10

    CPC分类号： C07K14/4705 ,  C07K14/4738

    摘要： Cell cultures are described which proliferate in a serum- and protein-free environment and which are characterized by the circumstance that the concentration of at least one cell-cycle-regulatory protein is increased during at least one period of the cell-cycle. Methods for the production of such cell cultures are presented. With these methods cell lines can be transferred to serum- and protein-free growth that are already in use in a production process which, prior to the use of these methods, requires serum or proteins added to the basal medium.

    摘要翻译： 描述了在无血清和无蛋白质环境中增殖的细胞培养物，其特征在于至少一种细胞周期调节蛋白的浓度在细胞周期的至少一个时期内增加。 提出了生产这种细胞培养物的方法。 通过这些方法，可以将细胞系转移到已经在生产过程中使用的无血清和无蛋白质生长，其在使用这些方法之前需要将血清或蛋白质添加到基础培养基中。

    Expression cloning processes for the discovery, characterization and isolation of genes encoding polypeptides with a predetermined property
    3.
    发明授权
    Expression cloning processes for the discovery, characterization and isolation of genes encoding polypeptides with a predetermined property 失效
    用于发现，表征和分离编码具有预定特性的多肽的基因的表达克隆方法

    公开(公告)号：US06524792B1

    公开(公告)日：2003-02-25

    申请号：US09193707

    申请日：1998-11-17

    申请人： Wolfgang A. Renner ,  Georg H. Orberger ,  Daniel Koller ,  James E. Bailey

    发明人： Wolfgang A. Renner ,  Georg H. Orberger ,  Daniel Koller ,  James E. Bailey

    IPC分类号： C12Q168

    CPC分类号： C12N15/1086 ,  C12Q1/6897 ,  Y10S435/911 ,  Y10S435/912

    摘要： The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.

    摘要翻译： 本发明涉及用于检测，表征和分离编码具有所需性质（例如特定细胞定位）的蛋白质的核酸的方法。 本发明进一步提供了这些蛋白质或糖蛋白在哺乳动物细胞中的快速表达，并促进新分泌的蛋白质或糖蛋白的纯化。 此外，本发明提供新型蛋白质或糖蛋白的放射性标记，用于快速鉴定包括动物在内的结合位点，并快速产生用于基因转移的感染性病毒载体。

    Expression cloning processes for the discovery characterization, and isolation of genes encoding polypeptides with a predetermined property
    4.
    发明授权
    Expression cloning processes for the discovery characterization, and isolation of genes encoding polypeptides with a predetermined property 失效
    用于发现表征的表达克隆过程，以及分离编码具有预定特性的多肽的基因

    公开(公告)号：US06197502B1

    公开(公告)日：2001-03-06

    申请号：US08972218

    申请日：1997-11-17

    申请人： Wolfgang A. Renner ,  Georg H. Orberger ,  Daniel Koller ,  James E. Bailey

    发明人： Wolfgang A. Renner ,  Georg H. Orberger ,  Daniel Koller ,  James E. Bailey

    IPC分类号： C12Q168

    CPC分类号： C12N15/1086 ,  C12Q1/6897 ,  Y10S435/911 ,  Y10S435/912

    摘要： The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.

    摘要翻译： 本发明涉及用于检测，表征和分离编码具有所需性质（例如特定细胞定位）的蛋白质的核酸的方法。 本发明进一步提供了这些蛋白质或糖蛋白在哺乳动物细胞中的快速表达，并促进新分泌的蛋白质或糖蛋白的纯化。 此外，本发明提供新型蛋白质或糖蛋白的放射性标记，用于快速鉴定包括动物在内的结合位点，并快速产生用于基因转移的感染性病毒载体。

    Inducible alphaviral gene expression system
    9.
    发明授权
    Inducible alphaviral gene expression system 失效
    诱导性甲病毒基因表达系统

    公开(公告)号：US07005275B2

    公开(公告)日：2006-02-28

    申请号：US09275883

    申请日：1999-03-25

    申请人： Wolfgang A. Renner ,  Lars Nieba ,  Marco Boorsma

    发明人： Wolfgang A. Renner ,  Lars Nieba ,  Marco Boorsma

    IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C07K14/005 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/127 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2840/105

    摘要： The present invention provides novel expression vectors which permit tight regulation of gene expression in eucaryotic cells. More specifically, the invention provides DNA vectors comprising nucleotide sequences that are transcribed to form RNA molecules which are then replicated by a temperature-sensitive replicase to form additional RNA molecules. The RNA molecules produced by replication contain a nucleotide sequence which may be translated to produce a protein of interest or which encode one or more untranslated RNA molecules. Also provided are methods for producing heterologous proteins and untranslated RNA molecules. Further provided are methods for administering heterologous proteins and untranslated RNA molecules to individuals. In addition, pharmaceutical compositions are provided comprising the DNA and RNA molecules of the invention and a pharmaceutically acceptable carrier.

    摘要翻译： 本发明提供了允许在真核细胞中严格调控基因表达的新型表达载体。 更具体地，本发明提供了包含被转录以形成RNA分子的核苷酸序列的DNA载体，然后由分子复制酶形成额外的RNA分子。 通过复制产生的RNA分子含有可翻译成产生目的蛋白或编码一个或多个未翻译的RNA分子的核苷酸序列。 还提供了用于产生异源蛋白质和非翻译RNA分子的方法。 还提供了向个体施用异源蛋白质和非翻译的RNA分子的方法。 此外，提供了包含本发明的DNA和RNA分子和药学上可接受的载体的药物组合物。