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    PACKAGED VIRUS-LIKE PARTICLES FOR USE AS ADJUVANTS: METHOD OF PREPARATION AND USE
    3.
    发明申请
    PACKAGED VIRUS-LIKE PARTICLES FOR USE AS ADJUVANTS: METHOD OF PREPARATION AND USE 审中-公开
    用作ADJUVANTS的包装的病毒颗粒：制备和使用方法

    公开(公告)号：US20110070267A1

    公开(公告)日：2011-03-24

    申请号：US12790708

    申请日：2010-05-28

    申请人： Martin F. Bachmann ,  Wolfgang A. Renner

    发明人： Martin F. Bachmann ,  Wolfgang A. Renner

    IPC分类号： A61K39/35 ,  A61K39/36 ,  A61P37/04 ,  A61P37/08

    CPC分类号： C12N7/00 ,  A61K39/0011 ,  A61K39/35 ,  A61K39/385 ,  A61K39/39 ,  A61K2039/5258 ,  A61K2039/55516 ,  A61K2039/55561 ,  A61K2039/55588 ,  A61K2039/57 ,  A61K2039/6075 ,  C07K14/005 ,  C12N2730/10122 ,  C12N2730/10123 ,  Y02A50/467

    摘要： The invention relates to the finding that virus like particles (VLPs) can be loaded and packaged, respectively, with DNA oligonucleotides rich in non-methylated C and G (CpGs). If such CpG-VLPs are mixed with antigens, the immunogenicity of these antigens are dramatically enhanced. In addition, the T cell responses against the antigens are especially directed to the Th1 type. Surprisingly, no covalent linkage of the antigen to the VLP is required; it is sufficient to simply mix the VLPs with the adjuvants for co-administration. In addition, it was found that VLPs did not enhance immune responses unless they were loaded and packaged, respectively, with CpGs. Antigens mixed with CpG-packaged VLPs may therefore be ideal vaccines for prophylactic or therapeutic vaccination against allergies, tumors and other self-molecules and chronic viral diseases.

    摘要翻译： 本发明涉及可以分别用富含非甲基化C和G（CpG）的DNA寡核苷酸装载和包装病毒样颗粒（VLPs）的发现。 如果这种CpG-VLP与抗原混合，则这些抗原的免疫原性显着增强。 此外，针对抗原的T细胞应答特别针对Th1型。 令人惊讶的是，不需要抗原与VLP的共价连接; 简单地将VLP与用于共同给药的佐剂混合就足够了。 此外，发现VLP除了分别用CpG装载和包装外，还没有增强免疫应答。 因此，与CpG包装的VLP混合的抗原可能是针对过敏，肿瘤和其他自身分子和慢性病毒性疾病的预防性或治疗性接种疫苗的理想疫苗。

    Inducible alphaviral gene expression system
    7.
    发明授权
    Inducible alphaviral gene expression system 失效
    诱导性甲病毒基因表达系统

    公开(公告)号：US07005275B2

    公开(公告)日：2006-02-28

    申请号：US09275883

    申请日：1999-03-25

    申请人： Wolfgang A. Renner ,  Lars Nieba ,  Marco Boorsma

    发明人： Wolfgang A. Renner ,  Lars Nieba ,  Marco Boorsma

    IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C07K14/005 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/127 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2840/105

    摘要： The present invention provides novel expression vectors which permit tight regulation of gene expression in eucaryotic cells. More specifically, the invention provides DNA vectors comprising nucleotide sequences that are transcribed to form RNA molecules which are then replicated by a temperature-sensitive replicase to form additional RNA molecules. The RNA molecules produced by replication contain a nucleotide sequence which may be translated to produce a protein of interest or which encode one or more untranslated RNA molecules. Also provided are methods for producing heterologous proteins and untranslated RNA molecules. Further provided are methods for administering heterologous proteins and untranslated RNA molecules to individuals. In addition, pharmaceutical compositions are provided comprising the DNA and RNA molecules of the invention and a pharmaceutically acceptable carrier.

    摘要翻译： 本发明提供了允许在真核细胞中严格调控基因表达的新型表达载体。 更具体地，本发明提供了包含被转录以形成RNA分子的核苷酸序列的DNA载体，然后由分子复制酶形成额外的RNA分子。 通过复制产生的RNA分子含有可翻译成产生目的蛋白或编码一个或多个未翻译的RNA分子的核苷酸序列。 还提供了用于产生异源蛋白质和非翻译RNA分子的方法。 还提供了向个体施用异源蛋白质和非翻译的RNA分子的方法。 此外，提供了包含本发明的DNA和RNA分子和药学上可接受的载体的药物组合物。

    Methods for the activation of proliferation of animal cells
    10.
    发明授权
    Methods for the activation of proliferation of animal cells 失效
    激活动物细胞增殖的方法

    公开(公告)号：US5811299A

    公开(公告)日：1998-09-22

    申请号：US385142

    申请日：1995-02-07

    申请人： Wolfgang A. Renner ,  Hans M. Eppenberger ,  James E. Bailey

    发明人： Wolfgang A. Renner ,  Hans M. Eppenberger ,  James E. Bailey

    IPC分类号： C07K14/47 ,  C12N5/06 ,  C12N5/10

    CPC分类号： C07K14/4705 ,  C07K14/4738

    摘要： Cell cultures are described which proliferate in a serum- and protein-free environment and which are characterized by the circumstance that the concentration of at least one cell-cycle-regulatory protein is increased during at least one period of the cell-cycle. Methods for the production of such cell cultures are presented. With these methods cell lines can be transferred to serum- and protein-free growth that are already in use in a production process which, prior to the use of these methods, requires serum or proteins added to the basal medium.

    摘要翻译： 描述了在无血清和无蛋白质环境中增殖的细胞培养物，其特征在于至少一种细胞周期调节蛋白的浓度在细胞周期的至少一个时期内增加。 提出了生产这种细胞培养物的方法。 通过这些方法，可以将细胞系转移到已经在生产过程中使用的无血清和无蛋白质生长，其在使用这些方法之前需要将血清或蛋白质添加到基础培养基中。