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    Phage display using cotranslational translocation of fusion polypeptides
    2.
    发明授权
    Phage display using cotranslational translocation of fusion polypeptides 有权
    噬菌体展示使用融合多态的转译易位

    公开(公告)号：US08846577B2

    公开(公告)日：2014-09-30

    申请号：US11988389

    申请日：2006-06-30

    申请人： Daniel Steiner ,  Patrik Forrer ,  Michael T. Stumpp ,  Andreas Plückthun

    发明人： Daniel Steiner ,  Patrik Forrer ,  Michael T. Stumpp ,  Andreas Plückthun

    IPC分类号： C40B50/06 ,  C12N15/10

    CPC分类号： C07K1/047 ,  C07K2319/034 ,  C07K2319/43 ,  C07K2319/50 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/625 ,  C12N15/63 ,  C40B40/02

    摘要： The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation.

    摘要翻译： 本发明涉及一种丝状噬菌体展示法，其中显示在噬菌体颗粒上的感兴趣多肽基于信号识别颗粒途径共转移穿过革兰氏阴性细菌的细胞质膜。 该方法特别适用于已知难以在噬菌体上显示的多肽，以及cDNA文库和其他组合文库的蛋白质，特别是当从非常快的折叠的稳定的蛋白质支架衍生时。 本发明还涉及可用于包含编码融合多肽的基因构建体的噬菌体或噬菌粒载体，所述融合多肽包含要显示在噬菌体颗粒上的多肽和促进共翻译易位的N末端信号序列。

    DESIGNED ARMADILLO REPEAT PROTEINS
    3.
    发明申请
    DESIGNED ARMADILLO REPEAT PROTEINS 有权
    设计ARMADILLO REPEAT蛋白

    公开(公告)号：US20110224100A1

    公开(公告)日：2011-09-15

    申请号：US12733836

    申请日：2008-09-21

    申请人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

    发明人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

    IPC分类号： C40B40/08 ,  C40B40/10 ,  C07K14/435 ,  C07K1/14

    CPC分类号： C07K14/4703 ,  C12N15/1044

    摘要： The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.

    摘要翻译： 本发明涉及基于犰狳重复蛋白的靶特异性设计的结合蛋白的集合，以及生成它们的方法。 设计的犰狳重复蛋白基于单犰狳重复单位的共有序列。 这些重复蛋白质可用作肽识别的支架。 这样的支架提供了对于每个小的可识别单元原则上相同的装订模式，例如， 氨基酸或二肽，允许在扩展构象中精确和模块识别肽。 该方法允许产生识别这些简单单元的一系列模块，并且组合这些构建块以产生任何所需肽靶的结合位点，而不进行附加选择。

    Protein/(poly)peptide libraries
    4.
    发明授权
    Protein/(poly)peptide libraries 有权
    蛋白质/（多）肽文库

    公开(公告)号：US06696248B1

    公开(公告)日：2004-02-24

    申请号：US09490070

    申请日：2000-01-24

    申请人： Achim Knappik ,  Peter Pack ,  Liming Ge ,  Simon Moroney ,  Andreas Plückthun

    发明人： Achim Knappik ,  Peter Pack ,  Liming Ge ,  Simon Moroney ,  Andreas Plückthun

    IPC分类号： C12Q168

    CPC分类号： C40B40/02 ,  C07K1/047 ,  C07K16/00 ,  C07K2319/00 ,  C12N15/10 ,  C12N15/1037

    摘要： The present invention relates to synthetic DNA sequences which encode one or more collections of homologous proteins/(poly)peptides, and methods for generating and applying libraries of these DNA sequences. In particular, the invention relates to the preparation of a library of human-derived antibody genes by the use of synthetic consensus sequences which cover the structural repertoire of antibodies encoded in the human genome. Furthermore, the invention relates to the use of a single consensus antibody gene as a universal framework for highly diverse antibody libraries.

    摘要翻译： 本发明涉及编码一个或多个同源蛋白/（多）肽集合的合成DNA序列，以及用于产生和应用这些DNA序列的文库的方法。 特别地，本发明涉及通过使用覆盖人类基因组中编码的抗体结构谱的合成共有序列来制备人源抗体基因文库。 此外，本发明涉及单一共有抗体基因作为高度多样化抗体文库的通用框架的用途。

    Methods for obtaining, identifying and applying nucleic acid sequences and (poly)peptides which increase the expression yields of periplasmic proteins in functional form
    5.
    发明授权
    Methods for obtaining, identifying and applying nucleic acid sequences and (poly)peptides which increase the expression yields of periplasmic proteins in functional form 失效
    用于获得，鉴定和应用核酸序列和（多）肽的方法，其增加功能形式的周质蛋白的表达产率

    公开(公告)号：US06630317B1

    公开(公告)日：2003-10-07

    申请号：US09564351

    申请日：2000-05-01

    申请人： Andreas Plückthun ,  Hendrick Bothmann

    发明人： Andreas Plückthun ,  Hendrick Bothmann

    IPC分类号： G01N33573

    CPC分类号： C40B40/02 ,  C07K14/245 ,  C07K16/00 ,  C12N15/1037 ,  C12N15/70 ,  C12Q1/6811

    摘要： The present invention relates to a method for obtaining nucleic acid sequences encoding (poly)peptides which increase the expression yields of periplasmic proteins in functional form upon co-expression of said (poly)peptides and said periplasmic proteins. The invention also provides a method for the identification of said (poly)peptides. Furthermore, the present invention relates to a method for increasing the expression yields of periplasmic proteins in functional form by co-expressing (poly)peptides, for example Skp, FkpA, or a homolog of Skp or FkpA, in bacteria.

    摘要翻译： 本发明涉及用于获得编码（多）肽的核酸序列的方法，其在共表达所述（多）肽和周质蛋白时增加功能形式的周质蛋白的表达产率。 本发明还提供了鉴定所述（多）肽的方法。 此外，本发明涉及通过在细菌中共表达（多）肽，例如Skp，FkpA或Skp或FkpA的同源物来增加功能形式的周质蛋白的表达产率的方法。

    Polysome display in the absence of functional ssrA-RNA
    6.
    发明授权
    Polysome display in the absence of functional ssrA-RNA 有权
    不存在功能性ssrA-RNA的Polysome显示

    公开(公告)号：US06348315B1

    公开(公告)日：2002-02-19

    申请号：US09425585

    申请日：1999-10-22

    申请人： Andreas Plückthun ,  Jozef Hanes ,  Lutz Jermutus

    发明人： Andreas Plückthun ,  Jozef Hanes ,  Lutz Jermutus

    IPC分类号： C12Q168

    CPC分类号： C12N15/1041 ,  C07K16/00

    摘要： The present invention relates to methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules. The method of the present invention is particularly characterized by an in vitro translation step under conditions that allow formation of polysomes in the presence of antisense oligonucleotides complementary to the tag-coding sequence of ssrA-RNA. The present invention further relates to kits that are useful for carrying out the method of the invention.

    摘要翻译： 本发明涉及用于鉴定与靶分子相互作用的编码（多）肽的核酸分子的方法。 在允许在与ssrA-RNA的标签编码序列互补的反义寡核苷酸存在下形成多核糖体的条件下，本发明的方法的特征在于体外翻译步骤。 本发明还涉及可用于实施本发明方法的试剂盒。

    Designed armadillo repeat proteins
    7.
    发明授权
    Designed armadillo repeat proteins 有权
    设计犰狳重复蛋白

    公开(公告)号：US09365629B2

    公开(公告)日：2016-06-14

    申请号：US12733836

    申请日：2008-09-23

    申请人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

    发明人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

    IPC分类号： C40B40/10 ,  C07K14/47 ,  C12N15/10

    CPC分类号： C07K14/4703 ,  C12N15/1044

    摘要： The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.

    摘要翻译： 本发明涉及基于犰狳重复蛋白的靶特异性设计的结合蛋白的集合，以及生成它们的方法。 设计的犰狳重复蛋白基于单犰狳重复单位的共有序列。 这些重复蛋白质可用作肽识别的支架。 这样的支架提供了对于每个小的可识别单元原则上相同的装订模式，例如， 氨基酸或二肽，允许在扩展构象中精确和模块识别肽。 该方法允许产生识别这些简单单元的一系列模块，并且组合这些构建块以产生任何所需肽靶的结合位点，而不进行附加选择。

    Immunoglobulin superfamily domains and fragments with increased solubility
    10.
    发明授权
    Immunoglobulin superfamily domains and fragments with increased solubility 有权
    免疫球蛋白超家族结构域和溶解度增加的片段

    公开(公告)号：US06815540B1

    公开(公告)日：2004-11-09

    申请号：US09232290

    申请日：1999-01-15

    申请人： Andreas Plückthun ,  Lars Nieba ,  Annemarie Honegger

    发明人： Andreas Plückthun ,  Lars Nieba ,  Annemarie Honegger

    IPC分类号： C07H2104

    CPC分类号： C07K16/00 ,  C07K16/44 ,  C07K2319/00

    摘要： The present invention relates to the modification of immunoglobulin superfamily (IgSF) domains, IgSF fragments and fusion proteins thereof, especially to the modification of antibody derivatives, so as to improve their solubility, and hence the yield, and ease of handling. The inventors have found that this can be achieved by making the region which comprised the interface with domains adjoined to said IgSF domain in a larger fragment or a full IgSF protein, and which becomes exposed in the IgSF domain, more hydrophilic by modification. The present invention describes DNA sequences encoding modified IgSF domains or fragments and fusion proteins thereof, vectors and hosts containing these DNA sequences, IgSF domains or fragments or fusion proteins obtainable by expressing said DNA sequences in suitable expression systems, and a method for modifying IgSF domains, so as to improve their solubility, expressibility and ease of handling.