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    NUCLEIC ACID SEQUENCING
    1.
    发明申请
    NUCLEIC ACID SEQUENCING 有权
    核酸序列

    公开(公告)号：US20120046176A1

    公开(公告)日：2012-02-23

    申请号：US12860462

    申请日：2010-08-20

    申请人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

    发明人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

    IPC分类号： C40B20/00

    CPC分类号： C12Q1/6874 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2565/301 ,  C12Q2565/607

    摘要： Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.

    摘要翻译： 提供使用DNA连接子的核酸测序。 任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。 还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。 使用检测器阵列电子和/或光学检测核酸测序反应。

    Nucleic acid sequencing
    2.
    发明授权
    Nucleic acid sequencing 有权
    核酸测序

    公开(公告)号：US08715932B2

    公开(公告)日：2014-05-06

    申请号：US12860462

    申请日：2010-08-20

    申请人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

    发明人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2565/301 ,  C12Q2565/607

    摘要： Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.

    摘要翻译： 提供使用DNA连接子的核酸测序。 任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。 还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。 使用检测器阵列电子和/或光学检测核酸测序反应。

    Chemically induced optical signals and DNA sequencing
    3.
    发明申请
    Chemically induced optical signals and DNA sequencing 有权
    化学诱导光信号和DNA测序

    公开(公告)号：US20100330553A1

    公开(公告)日：2010-12-30

    申请号：US12459309

    申请日：2009-06-30

    申请人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    发明人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    IPC分类号： C12Q1/68 ,  C12M1/00 ,  G02B6/10

    CPC分类号： G02B6/1221 ,  C12Q1/6874 ,  C12Q2565/607 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.

    摘要翻译： 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物附着的螯合剂捕获并光学检测以确定待测序的DNA分子中下一个互补核酸的同一性。

    Chemically induced optical signals and DNA sequencing
    4.
    发明授权
    Chemically induced optical signals and DNA sequencing 有权

    公开(公告)号：US09671558B2

    公开(公告)日：2017-06-06

    申请号：US12459309

    申请日：2009-06-30

    申请人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    发明人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    IPC分类号： C12Q1/68 ,  G02B6/122

    CPC分类号： G02B6/1221 ,  C12Q1/6874 ,  C12Q2565/607 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.

    Nucleotides and oligonucleotides for nucleic acid sequencing
    7.
    发明授权
    Nucleotides and oligonucleotides for nucleic acid sequencing 有权
    用于核酸测序的核苷酸和寡核苷酸

    公开(公告)号：US08674086B2

    公开(公告)日：2014-03-18

    申请号：US12823995

    申请日：2010-06-25

    申请人： Jianquan Liu ,  Xing Su ,  Kai Wu

    发明人： Jianquan Liu ,  Xing Su ,  Kai Wu

    IPC分类号： C07H19/20 ,  C07H19/00 ,  C07H19/04

    CPC分类号： C07H19/10 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2521/319 ,  C12Q2521/525 ,  C12Q2525/186 ,  C12Q2537/157 ,  C12Q2565/537 ,  C12Q2565/607

    摘要： Embodiments of the invention provide non-natural bifunctional nucleotides having both nuclease resistance and nucleic acid synthesis blocking properties and methods of sequencing nucleic acids that employ non-natural bifunctional nucleic acids. Additional embodiments provide non-natural oligonucleotides and methods for sequencing nucleic acids using the non-natural oligonucleotides. Methods according to embodiments of the invention employ electronic detection and fluorescent detection of nucleic acid sequencing reactions.

    摘要翻译： 本发明的实施方案提供具有核酸酶抗性和核酸合成阻断性质的非天然双功能核苷酸，以及使用非天然双功能核酸测序核酸的方法。 另外的实施方案提供非天然寡核苷酸和使用非天然寡核苷酸测序核酸的方法。 根据本发明的实施方案的方法使用核酸测序反应的电子检测和荧光检测。

    DEVICE AND METHOD FOR DETECTING REDOX REACTIONS IN SOLUTION
    8.
    发明申请
    DEVICE AND METHOD FOR DETECTING REDOX REACTIONS IN SOLUTION 有权
    用于检测溶液中的氧化还原反应的装置和方法

    公开(公告)号：US20140083871A1

    公开(公告)日：2014-03-27

    申请号：US13628728

    申请日：2012-09-27

    申请人： Jonathan S. Daniels ,  Oguz H. Elibol ,  Grace M. Credo ,  Xing Su

    发明人： Jonathan S. Daniels ,  Oguz H. Elibol ,  Grace M. Credo ,  Xing Su

    IPC分类号： G01N27/30 ,  B82Y30/00

    CPC分类号： G01N27/3277 ,  B82Y30/00 ,  C12Q1/68 ,  G01D5/24 ,  G01K7/34 ,  G01L1/14 ,  G01N27/22 ,  G01R27/26 ,  G06K7/08

    摘要： Described herein is a device comprising a plurality of first reaction electrodes arranged in an array, the plurality of first reaction electrodes configured to be exposed to a solution and having a capacitance; first circuitry configured to controllably connect the plurality of first reaction electrodes to a bias source and controllably disconnect the plurality of first reaction electrodes from the bias source; and second circuitry configured to measure a rate of charging or discharging of the capacitance. Also described herein is a method of using this device to sequence DNA.

    摘要翻译： 这里描述的是一种装置，其包括排列成阵列的多个第一反应电极，多个第一反应电极构造为暴露于溶液并具有电容; 第一电路，被配置为将多个第一反应电极可控地连接到偏置源并且可控制地将多个第一反应电极与偏置源分离; 以及第二电路，被配置为测量电容的充电或放电速率。 本文还描述了使用该装置来对DNA进行序列化的方法。

    Nanogap chemical and biochemical sensors
    9.
    发明申请
    Nanogap chemical and biochemical sensors 有权
    Nanogap化学和生化传感器

    公开(公告)号：US20110155586A1

    公开(公告)日：2011-06-30

    申请号：US12655578

    申请日：2009-12-31

    申请人： Oguz H. Elibol ,  Jonathan S. Daniels ,  Grace M. Credo ,  Xing Su

    发明人： Oguz H. Elibol ,  Jonathan S. Daniels ,  Grace M. Credo ,  Xing Su

    IPC分类号： C12Q1/25 ,  G01N27/26 ,  C12Q1/42

    CPC分类号： G01N27/3278

    摘要： Devices and methods are presented for electronic sensing of chemical and biochemical analytes. An electronic sensor having a at least two electrodes separated by a nanoscale gap wherein the separation between the first electrode and the second electrode forms a cavity capable of containing a fluid wherein two or more posts comprised of an insulating material extend into the cavity from the face of the first electrode to the face of the second electrode. Optionally, the cavity is closed with a bead. Devices according to embodiments of the invention are capable of detecting chemicals and biochemicals through redox cycling events. Additionally, devices and methods according to embodiments of the invention are adapted to identify and sequence nucleic acid molecules.

    摘要翻译： 介绍了化学和生物化学分析物的电子感应装置和方法。 一种电子传感器，具有由纳米尺度间隙分开的至少两个电极，其中第一电极和第二电极之间的间隔形成能够容纳流体的空腔，其中由绝缘材料构成的两个或更多个柱从表面延伸到空腔中 的第一电极到第二电极的表面。 可选地，空腔用珠封闭。 根据本发明的实施例的装置能够通过氧化还原循环事件来检测化学品和生物化学品。 另外，根据本发明的实施方案的装置和方法适于鉴定和序列核酸分子。