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    LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS
    8.
    发明申请
    LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS 审中-公开
    与无机磷离子通道相关的无标记方法

    公开(公告)号：US20130040331A1

    公开(公告)日：2013-02-14

    申请号：US13519246

    申请日：2010-12-16

    申请人： Ye Fang ,  Haiyan Sun

    发明人： Ye Fang ,  Haiyan Sun

    IPC分类号： C12Q1/02 ,  G01N27/26

    CPC分类号： G01N33/6872 ,  G01N2500/10

    摘要： Disclosed are methods to classify human ether-à-go-go related gene (hERG) ion channel modulators using label-free biosensors. Disclosed is the use of label-free resonant waveguide grating (RWG) biosensors to reveal the patterns of the DMR signals of hERG modulators across three types of cell lines (a native cell line endogenously expressing hERG, a native cell line without hERG and its engineered cell line stably expressed hERG), as well as the corresponding modulation index of the modulators molecules against a hERG activator acting on a panel of markers/cells, particularly the known hERG activator mallotoxin DMR signals in the two hERG expressing cell lines.

    摘要翻译： 公开了使用无标记生物传感器分类人类醚转运相关基因（hERG）离子通道调节剂的方法。 公开了使用无标记谐振波导光栅（RWG）生物传感器来显示三种类型的细胞系（内源性表达hERG的天然细胞系，没有hERG的天然细胞系的天然细胞系及其工程化的hERG调节剂的DMR信号的模式 细胞系稳定表达hERG），以及调节剂分子对作用于一组标志物/细胞的hERG激活剂的相应调节指数，特别是两种hERG表达细胞系中已知的hERG活化剂mallotoxin DMR信号。

    Method and device for protein delivery into cells
    10.
    发明授权
    Method and device for protein delivery into cells 失效

    公开(公告)号：US08293484B2

    公开(公告)日：2012-10-23

    申请号：US12893389

    申请日：2010-09-29

    申请人： Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

    发明人： Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

    IPC分类号： G01N33/53

    CPC分类号： C12N5/0068 ,  C12N2503/00 ,  C12N2535/10

    摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.