公开(公告)号：US20160298103A1

公开(公告)日：2016-10-13

申请号：US15189021

申请日：2016-06-22

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron MILO ,  Arren BAR-EVEN ,  Elad NOOR

IPC: C12N9/00 ,  C12P7/46 ,  C12N15/70 ,  C12N9/10 ,  C12N9/88 ,  C12P11/00 ,  C12P7/42

CPC classification number: C12N9/93 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8269 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P11/00 ,  C12Y101/01059 ,  C12Y102/01018 ,  C12Y201/03001 ,  C12Y208/03001 ,  C12Y401/01031 ,  C12Y402/01017 ,  C12Y402/01054 ,  C12Y604/01001 ,  C12Y604/01002 ,  G06F19/12 ,  Y02P20/52

Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

Abstract translation: 提供了一种用于碳固定的系统。 该系统包括催化碳固定通道的反应的酶，其中碳固定通路的至少一个反应是羧化反应，其中碳固定路径的反应产物包括草酰乙酸酯和丙二酰辅酶A，其中酶 进行羧化反应的选自苯丙醇丙酮酸（PEP）羧基酶，丙酮酸羧化酶和乙酰​​辅酶A羧化酶，其中碳固定路径的出口产物是乙醛酸盐。 还提供了另外的碳固定路径和产生它们的方法。

公开(公告)号：US20190211342A1

公开(公告)日：2019-07-11

申请号：US16362897

申请日：2019-03-25

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron MILO ,  Niv ANTONOVSKY ,  Elad NOOR ,  Arren BAR-EVEN ,  Yehudit ZOHAR ,  Lior ZELCBUCH ,  Shmuel GLEIZER ,  Shira AMRAM

IPC: C12N15/70 ,  C07K14/195 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C07K14/245 ,  C12N1/20

CPC classification number: C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01049 ,  C12Y203/03009 ,  C12Y207/01011 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01011 ,  C12Y504/02

Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.

公开(公告)号：US20240117386A1

公开(公告)日：2024-04-11

申请号：US17768228

申请日：2020-10-22

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron MILO ,  Shmuel GLEIZER ,  Niv ANTONOVSKY ,  Elad NOOR ,  Arren BAR-EVEN ,  Yehudit ZOHAR ,  Roee BEN NISSAN ,  Elad HERZ ,  Yinon Moise BAR-ON

IPC: C12P1/04 ,  C07K14/195 ,  C12N1/20 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88

CPC classification number: C12P1/04 ,  C07K14/195 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/1205 ,  C12N9/88 ,  C12Y102/02001 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01001

Abstract: A recombinant bacteria which is genetically modified to express formate dehydrogenase (FDH), phosphoribulokinase (prk) and Ribulose-Bisphosphate Carboxylase/oxygenase (RuBisCo) is disclosed. The bacteria may be modified to be autotrophic.

公开(公告)号：US20170183665A1

公开(公告)日：2017-06-29

申请号：US15312734

申请日：2015-05-21

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron MILO ,  Niv ANTONOVSKY ,  Elad NOOR ,  Arren BAR-EVEN ,  Yehudit ZOHAR ,  Lior ZELCBUCH ,  Shmuel GLEIZER ,  Shira AMRAM

IPC: C12N15/70 ,  C12N9/88 ,  C12N1/20 ,  C12N9/90 ,  C07K14/245 ,  C12N9/10 ,  C12N9/12 ,  C12N9/04

CPC classification number: C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01049 ,  C12Y203/03009 ,  C12Y207/01011 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01011 ,  C12Y504/02

Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.

公开(公告)号：US20170081682A1

公开(公告)日：2017-03-23

申请号：US15101097

申请日：2014-12-10

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren BAR-EVEN ,  Ron MILO ,  Elad NOOR ,  Lior ZELCBUCH

IPC: C12P7/40 ,  C12N1/20 ,  C12N9/10 ,  C12P7/26 ,  C12P7/16 ,  C12P5/02 ,  C12P5/00 ,  C12P7/64 ,  C12N15/52 ,  C12P7/06

CPC classification number: C12P7/40 ,  C12N1/20 ,  C12N9/1029 ,  C12N15/52 ,  C12P5/007 ,  C12P5/023 ,  C12P5/026 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/649 ,  C12Y203/01054 ,  Y02E50/343

Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis.Uses of the microorganism and products comprising same are also disclosed.