摘要:
The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.
摘要:
The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译:本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸(PGA)。 根据本发明的超高分子量PGA的平均分子量大于13000kDa,其分子的95%以上具有3,000至15,000kDa的分子量。 此外,它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。 根据本发明的超高分子量PGA显示出非常优异的吸湿性,保湿性,缓释性,矿物溶解性和吸水性,因此可用作新的高附加值材料 在各种应用中。
摘要:
The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
摘要翻译:本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i))i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法,使用硝酸盐呼吸显示低生长量的共生杆菌SC-1及其相对共生菌的生长测定方法以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此,在本发明中由共生杆菌(Toibii)SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂,其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸(L-DOPA)和L-色氨酸。
摘要:
Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.
摘要翻译:本文公开了醛缩酶基因启动子及其用途,更具体地,公开了源自干酪乳杆菌的醛缩酶基因的启动子,其具有SEQ ID NO:1的碱基序列,含有所述启动子的表达载体和转化的重组微生物 与所述表达载体。 用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质,因此可用作疫苗载体等。
摘要:
Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.
摘要翻译:本文公开了醛缩酶基因启动子及其用途,更具体地,公开了源自干酪乳杆菌的醛缩酶基因的启动子,其具有SEQ ID NO:1的碱基序列,含有所述启动子的表达载体和转化的重组微生物 与所述表达载体。 用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质,因此可用作疫苗载体等。
摘要:
The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
摘要翻译:本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i))i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法,使用硝酸盐呼吸显示低生长产量的共生杆菌SC-1及其相对共生菌的生长方法,以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此,在本发明中由共生杆菌(Toibii)SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂,其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸(L-DOPA)和L-色氨酸。
摘要:
The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
摘要翻译:本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i))i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法,使用硝酸盐呼吸显示低生长产量的共生杆菌SC-1及其相对共生菌的生长方法,以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此,在本发明中由共生杆菌(Toibii)SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂,其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸(L-DOPA)和L-色氨酸。
摘要:
The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so that the present invention will be very efficient in establishing expression systems for certain genomes and gene groups.
摘要:
A system and a method for providing a secured transmission through an authenticated encryption for each ONU in downlink transmission of an OLT in GPON are provided. The GPON system includes an OLT for generating a GTC downlink frame by receiving data from an external service provider and ONUs for receiving the GTC downlink frame from the OLT and processing the received GTC downlink frame. The OLT performs the authenticated encryption for the generated GTC downlink frame according to the ONU by including an authentication generator and the ONU determines whether the GTC downlink frame is allowed to be processed or not by checking the authentication of the received GTC downlink frame through an authentication checker.
摘要:
A mobile terminal executes a navigation function by receiving Transport Protocol Experts Group (TPEG) traffic information through a Digital Multimedia Broadcasting (DMB) network. This DMB-based mobile terminal receives and decodes TPEG data at a separate second processor different from a conventional first processor. Further, the DMB-based mobile terminal may execute a calculation of an optimum route at the second processor. The terminal and a related method reduce the processing load of the first processor, which causes a decrease in the response time to a user's navigation request and improves a user's convenience.